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Induction of oral dryness and endocannabinoid

Research Project

Project/Area Number 23592743
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Functional basic dentistry
Research InstitutionKyushu Dental College

Principal Investigator

INENAGA Kiyotoshi  九州歯科大学, 歯学部, 教授 (90131903)

Co-Investigator(Kenkyū-buntansha) ONO Kentaro  九州歯科大学, 歯学部, 准教授 (40316154)
HITOMI Suzuro  九州歯科大学, 歯学部, 助教 (70548924)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Keywords内因性カンナビノイド / 脳弓下器官 / 飲水行動 / エタノール / のどの渇き / 飲水 / ナトリウム摂取 / パッチクランプ / γアミノ酪酸
Research Abstract

This study was to clarify a relationship between endocannabinoid and thirst sensation. Male Wistar rats were used in the experiment. First, the effects of endocannabinoids on IPSCs of neurons of the subfornical organ (SFO), which is a thirst center of the brain, recorded from brain slice preparations were investigated with patch clamp method. The CB1 receptor agonist WIN 55,221-2 (WIN) decreased significantly the frequency but not the amplitude of IPSCs. The effect was disappeared with the CB1 antagonist. Next, the endocannabinoids on neural activity of the SFO were investigated with the multi-electrode array (MEA) method. Application of the endocannabinoid anandamide and WIN affected unit activities in only the small number of neurons. The i.c.v. injection of WIN had no effects on water and salt intakes. These suggest that while endocannabinoids affect GABA release in the SFO, they do not play any important role in induction of thirst sensation, if present, in the normal condition.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (25 results)

All 2013 2012 2011 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (15 results) Remarks (3 results)

  • [Journal Article] Endothelin Receptor-mediated Responses in Trigeminal Ganglion Neurons2013

    • Author(s)
      Yamamoto M, Ono K, Hitomi S, Harano N, Sago T, Yoshida M, Nunomaki M, Shiiba S, Watanabe S, Nakanishi O, Inenaga K.
    • Journal Title

      J Dental Res

      Volume: 92 Issue: 4 Pages: 335-339

    • DOI

      10.1177/0022034513478428

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Distinct effects of cevimeline and pilocarpine on salivary mechanisms, cardiovascular response and thirst sensation in rats2012

    • Author(s)
      Ono K, Inagaki T, Iida T, Wakasugi-Sato N, Hosokawa R, Inenaga K.
    • Journal Title

      Arch Oral Biol

      Volume: 57(4) Issue: 4 Pages: 421-428

    • DOI

      10.1016/j.archoralbio.2011.09.013

    • Related Report
      2013 Final Research Report 2012 Research-status Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Dopamine modulates neuronal excitability pre-and post-synaptically in the rat subfornical organ2012

    • Author(s)
      Miyahara N, Ono K, Hitomi S, Hirase M, Inenaga K.
    • Journal Title

      Brain Res

      Volume: 1447 Pages: 44-52

    • DOI

      10.1016/j.brainres.2012.01.063

    • Related Report
      2013 Final Research Report 2012 Research-status Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Distinct time courses of microglial and astrocytic hyper-activation and the glial contribution to pain hypersensitivity in a facial cancer model2012

    • Author(s)
      Sago T, Ono K, Harano N, Furuta-Hidaka K, Hitomi S, Nunomaki M, at al.(11名中2番目)
    • Journal Title

      Brain Res

      Volume: (in press) Pages: 70-80

    • DOI

      10.1016/j.brainres.2012.03.039

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Distinct mechanisms underlie the regulation of body fluid balance by neurokinin B and angiotensin II in the rat brain2011

    • Author(s)
      Asami R, Ono K, Nakanishi O, Inenaga K.
    • Journal Title

      Brain Res

      Volume: 1383 Pages: 179-186

    • DOI

      10.1016/j.brainres.2011.01.072

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Neuronal effects of neurokinin B on the rat subfornical organ2011

    • Author(s)
      Ono K, Asami R, Miyahara N, Nakanishi O, Inenaga K.
    • Journal Title

      Neuroreport

      Volume: 22(8) Issue: 8 Pages: 374-378

    • DOI

      10.1097/wnr.0b013e3283469645

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Nicotinic receptor agonist-induced salivation and its cellular mechanism in parotid acini of rats2011

    • Author(s)
      Iida T, Ono K, Inagaki T, Hosokawa R, Inenaga K.
    • Journal Title

      Auton Neurosci

      Volume: 161(1-2) Issue: 1-2 Pages: 81-86

    • DOI

      10.1016/j.autneu.2011.01.003

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 二日酔い時における喉の渇きのメカニ2013

    • Author(s)
      稲永清敏、神田修治、近藤智裕、田中慶太、山口高広、氏原泉、人見涼露、小野堅太郎
    • Organizer
      第90回日本生理学会
    • Place of Presentation
      船堀
    • Related Report
      2013 Final Research Report
  • [Presentation] エタノールおよびアセトアルデヒドのラット脳弓下器官ニューロンに対する作用2013

    • Author(s)
      氏原泉、人見涼露、小野堅太郎、柿木保明、稲永清敏
    • Organizer
      第90回日本生理学会
    • Place of Presentation
      船堀
    • Related Report
      2013 Final Research Report
  • [Presentation] 二日酔いをした時の喉の渇きはアセトアルデヒドが原因でおこる2013

    • Author(s)
      稲永清敏、氏原泉、人見涼露、小野堅太郎、蔵満幸子、神田修治、近藤智浩、田中慶太、山口高広、柿木保明
    • Organizer
      第73回九州歯科学会総会
    • Related Report
      2013 Final Research Report
  • [Presentation] A mechanism of thirst sensation in hangover2013

    • Author(s)
      Inenaga K, Ujihara I, Hitomi S, Ono K, Kakinoki Y
    • Organizer
      The 11^<th> Korea-Japan Joint Symposium of Brain Sciences, and Cardiac and Smooth Muscle Sciences
    • Place of Presentation
      浜松
    • Related Report
      2013 Final Research Report
  • [Presentation] エタノールおよびアセトアルデヒドの口渇中枢ニューロンに対する作用2013

    • Author(s)
      氏原泉、人見涼露、小野堅太郎、柿木保明、稲永清敏
    • Organizer
      第55回歯科基礎医学会
    • Place of Presentation
      岡山
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 二日酔いでの口渇感はアセトアルデヒドが原因でおこる2013

    • Author(s)
      稲永清敏、氏原泉、人見涼露、小野堅太郎、柿木保明
    • Organizer
      第55回歯科基礎医学会
    • Place of Presentation
      岡山
    • Related Report
      2013 Final Research Report
  • [Presentation] アセトアルデヒドがレニン・アンジオテンシン系を活性化し、水分・塩分摂取を誘発する2013

    • Author(s)
      氏原泉、人見涼露、小野堅太郎、柿木保明、稲永清敏
    • Organizer
      第64回西日本生理学会
    • Place of Presentation
      北九州
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] A mechanism of thirst sensation in hangover2013

    • Author(s)
      Inenaga K, Ujihara I, Hitomi S, Ono K, Kakinoki Y
    • Organizer
      The 11^<th> International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
  • [Presentation] A mechanism of thirst sensation in hangover2013

    • Author(s)
      Inenaga K, Ujihara I, Hitomi S, Ono K, Kakinoki Y.
    • Organizer
      The 11th Korea-Japan Joint Symposium
    • Place of Presentation
      浜松
    • Related Report
      2013 Annual Research Report
  • [Presentation] A mechanism of thirst sensation in hangover2013

    • Author(s)
      Inenaga K, Ujihara I, Hitomi S, Ono K, Kakinoki Y.
    • Organizer
      The 11th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception
    • Place of Presentation
      福岡
    • Related Report
      2013 Annual Research Report
  • [Presentation] ラットの水および塩分摂取行動に対するエタノールの作用2012

    • Author(s)
      稲永清敏、神田修治、近藤智裕、田中慶太、山口高広、氏原泉、人見涼露、小野堅太郎
    • Organizer
      第63回西日本生理学会
    • Place of Presentation
      大分
    • Year and Date
      2012-10-19
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] 体液調節に関与した中枢ニューロンのアルコールに対する応答2012

    • Author(s)
      稲永清敏、人見涼露、小野堅太郎
    • Organizer
      第54回歯科基礎医学会
    • Place of Presentation
      郡山
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] ドーパミンはD4受容体を介して脳弓下器官ニューロンを抑制する

    • Author(s)
      宮原宣高、小野堅太郎、人見涼露、稲永清敏
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      横浜
    • Related Report
      2011 Research-status Report
  • [Presentation] Dopamine inhibits neuronal excitability in the rat subfornical organ

    • Author(s)
      Inenaga K, Miyahara N, Ono K.
    • Organizer
      Neuroscience Meeting
    • Place of Presentation
      ワシントン
    • Related Report
      2011 Research-status Report
  • [Presentation] Pre-and post-synaptic modulation by dopamine in rat subfornical organ neurons

    • Author(s)
      Inenaga K, Miyahara N, Ono K, Hitomi S.
    • Organizer
      日韓合同シンポジウム
    • Place of Presentation
      慶州
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.kyu-dent.ac.jp/docs/research/researcher/

    • Related Report
      2013 Final Research Report
  • [Remarks] 九州歯科大学・研究者総覧

    • URL

      http://www.kyu-dent.ac.jp/docs/research/researcher/inenaga_kiyotoshi.pdf

    • Related Report
      2013 Annual Research Report
  • [Remarks] 研究者総覧

    • URL

      http://www.kyu-dent.ac.jp/researcher/INENAGA_KIYOTOSHI.pdf

    • Related Report
      2012 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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