| Project/Area Number |
23592745
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
NEZU Akihiro 北海道医療大学, 歯学部, 講師 (00305913)
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| Co-Investigator(Kenkyū-buntansha) |
TANIMURA Akihiko 北海道医療大学, 歯学部, 教授 (70217149)
MORITA Takao 北海道医療大学, 歯学部, 講師 (20326549)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | 唾液腺 / カルシウム応答 / 唾液分泌 / in vivo / イメージング / in vivoイメージング |
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| Research Abstract |
To investigate the relationships between Ca2+ responses and salivary secretion in living animals, we measured Ca2+ responses in the rat submandibular gland (SMG) and salivary secretion from the SMG in real-time. First, we measured sialogogue-induced Ca2+ responses and salivary secretions. Administration of various agonist induced increase in intracellular Ca2+ concentration ([Ca2+]i) throughout the Ca2+ sensor-expressed SMG. In addition, acetylcholine frequently shows Ca2+ oscillations in whole gland. We found that there is the difference among the agonist-induced spatio-temporal changes in [Ca2+]i in living animals. When salivary secretions were measured with Ca2+ responses using the micro pressure sensor, secretions occurred with a delay from the increase in the [Ca2+]i. The expression of Stim1, which is essential for the activation of Ca2+ entry, increased agonist-induced Ca2+ responses in SMG cells, therefore it may be a suitable gene therapy strategy for dry mouth in the future.
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