Identification and characterization of biofilm regulatory autolysins

• Project/Area Number: 23592746
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Iwate Medical University
• Principal Investigator: KATO Hirohisa 岩手医科大学, 歯学部, 教授 (60152740)
• Co-Investigator(Kenkyū-buntansha): TAMURA Haruki 岩手医科大学, 歯学部, 講師 (30316393)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 自己溶菌酵素 / 口腔レンサ球菌 / 遺伝子同定 / 溶菌選択性 / 機能解析 / 遺伝子検索 / バイオフィルム

Research Abstract

In this study, autolysin genes have been identified from oral streptococci. We found that AtlA slightly bound to type I collagen and did not bind to dextran. Furthermore, Asp655 and Asp747 were critical for lytic activity in Streptococcus criceti AtlA. Finally, the four studied autolysins failed to lyse cells of Streptococcus oralis ATCC 10557.

Research Products

• [Journal Article] Identification and characterization of an autolysin gene, atlA, from Streptococcus criceti. (2012)
  • Author(s): Tamura H, Yamada A, Kato H.
  • Journal Title: J Microbiol. Volume: 50 Issue: 5 Pages: 777-784
  • DOI: 10.1007/s12275-012-2187-1
  • Peer Reviewed
• [Journal Article] Characterization of <i>Streptococcus criceti</i> insertion sequence IS<i>Scr1</i> (2012)
  • Author(s): Tamura H, Yamada A, Kato H.
  • Journal Title: Genes & Genetic Systems Volume: 87 Issue: 3 Pages: 153-160
  • DOI: 10.1266/ggs.87.153
  • NAID: 10030945158
  • ISSN: 1341-7568, 1880-5779
  • Peer Reviewed
• [Presentation] Streptococcus cricetiデキストラン結合レクチンB遺伝子のStreptococcus mutansにおける解析 (2013)
  • Author(s): 田村晴希、山田ありさ、加藤裕久
  • Organizer: 第55回歯科基礎医学会学術大会
  • Place of Presentation: 岡山
  • Year and Date: 2013-09-22
• [Presentation] Streptococcus cricetiにおけるStreptococcus sobrinusデキストラン結合レクチン遺伝子ホモログdblA, dblBの同定 (2013)
  • Author(s): 田村晴希、山田ありさ、加藤裕久
  • Organizer: 第86回日本薬理学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2013-03-23
• [Presentation] Streptococcus criceti E49株の挿入配列ISScr1の挿入部位の同定 (2012)
  • Author(s): 田村晴希、山田ありさ、加藤裕久
  • Organizer: 第54回歯科基礎医学会学術大会
  • Place of Presentation: 郡山
  • Year and Date: 2012-09-16
• [Presentation] treptococcus cricetiのグルカン結合タンパク質GbpDの遺伝子解析 (2011)
  • Author(s): 田村晴希、山田ありさ、加藤裕久
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 岐阜
  • Year and Date: 2011-10-02
• [Presentation] Streptococcus cricetiのグルカン結合タンパク質GbpDの遺伝子解析 (2011)
  • Author(s): 田村晴希、山田ありさ、加藤裕久
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 岐阜
• [Presentation] Streptococcus cricetiデキストラン結合レクチンB遺伝子のStreptococcus mutansにおける解析
  • Author(s): 田村晴希、山田ありさ、加藤裕久
  • Organizer: 歯科基礎医学会
  • Place of Presentation: 岡山市
• [Presentation] Streptococcus cricetiにおけるStreptococcus sobrinus デキストラン結合レクチン遺伝子ホモログdblA, dblBの同定
  • Author(s): 田村晴希、山田ありさ、加藤裕久
  • Organizer: 第86回日本薬理学会年会
  • Place of Presentation: 福岡