Identification and characterization of biofilm regulatory autolysins
| Project/Area Number |
23592746
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Functional basic dentistry
|
|---|
| Research Institution | Iwate Medical University |
|---|
Principal Investigator |
KATO Hirohisa 岩手医科大学, 歯学部, 教授 (60152740)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TAMURA Haruki 岩手医科大学, 歯学部, 講師 (30316393)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
|
|---|
| Keywords | 自己溶菌酵素 / 口腔レンサ球菌 / 遺伝子同定 / 溶菌選択性 / 機能解析 / 遺伝子検索 / バイオフィルム |
|---|
| Research Abstract |
In this study, autolysin genes have been identified from oral streptococci. We found that AtlA slightly bound to type I collagen and did not bind to dextran. Furthermore, Asp655 and Asp747 were critical for lytic activity in Streptococcus criceti AtlA. Finally, the four studied autolysins failed to lyse cells of Streptococcus oralis ATCC 10557.
|
Report
(4 results)
Research Products
(9 results)
