Regulation of differentiation of hard-tissue-constituting cells by pH regulating proteins. Clarification of the mechanism and its application for regenerative medicine.

• Project/Area Number: 23592748
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Showa University
• Principal Investigator: KAMIJO Ryutaro 昭和大学, 歯学部, 教授 (70233939)
• Co-Investigator(Kenkyū-buntansha): KATAGIRI Takenobu 埼玉医科大学, ゲノム医学研究センター病態生理部門, 教授 (80245802) ; MIYAMOTO Yoichi 昭和大学, 歯学部口腔生化学講座, 准教授 (20295132) ; YAMADA Atsushi 昭和大学, 歯学部口腔生化学講座, 講師 (50407558)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: pH / 骨芽細胞 / 軟骨細胞 / 炭酸脱水酵素 / モノカルボン酸輸送体 / MCT / 細胞内pH / 細胞分化 / carbonic anhydrase / MCT-1

Research Abstract

We found that carbonic anhydrase-9 (CA9) is expressed in mouse chondrocytes and monocarboxylate transporter-1 (MCT1) in osteoblasts, respectively. Hence we investigated the roles of CA9 and MCT1 in differentiation of chondrocytes and osteoblasts, respectively. CA9 was expressed in proliferating but not in hypertrophic chondrocytes in the growth plates of mice. Suppression of CA9 expression by its siRNA induced the hypertrophic differentiation in mouse primary chondrocytes, indicating that CA9 has a role to suppress hypertrophic differentiation of chondrocytes. Suppression of the expression of MCT1 in mouse osteoblasts induced lowered expression of alkaline phosphatase (ALP), while lactate, an MCT1 substrate, augmented the expression of ALP in osteoblasts. We also found that MCT1 positively regulates phosphorylation and nuclear translocation of Smad1/5/8 in osteoblasts after stimulation by bone morphogenetic protein-2.

Research Products

• [Journal Article] Porphyromonas gingivalis-derived lysine gingipain enhances osteoclast differentiation induced by tumor necrosis factor and interleukin-1 but suppresses that by interleukin-17: Importance of proteolytic degradation of osteoprotegerin by lysine gingipain. (2014)
  • Author(s): Tomohito Akiyama, Yoichi Miyamoto, Kentaro Yoshimura, Atsushi Yamada, Masamichi Takami, Tetsuo Suzawa, Marie Hoshino, Takahisa Imamura, Chie Akiyama, Rika Yasuhara, Kenji Mishima, Toshifumi Maruyama, Chikara Kohda, Kazuo Tanaka, Jan Potempa, Hisataka Yasuda, Kazuyoshi Baba, Ryutaro Kamijo.
  • Journal Title: J.Biol.Chem. Volume: 289 Issue: 22 Pages: 1-35
  • DOI: 10.1074/jbc.m113.520510
  • Peer Reviewed / Open Access
• [Journal Article] Identification of gene expression profile of neural crest-derived cells isolated from submandibular glands of adult mice. (2014)
  • Author(s): Takahashi M, Suzawa T, Yamada A, Yamaguchi T, Mishima K, Osumi N, Maki K, Kamijo R
  • Journal Title: BBRC Volume: 30 Issue: 2 Pages: 1925-1937
  • DOI: 10.1016/j.bbrc.2014.02.130
  • Peer Reviewed
• [Journal Article] Nanomechanical properties and molecular structures of in vitro mineralized tissues on anodically oxidized titanium surfaces. (2014)
  • Author(s): Jang, I-. K., Tanaka, R., Wurihan, Suzuki, D., Shibata Y., Fujisawa, N., Tanimoto, Y., Ogura, K., Kamijo, R., Miyazaki, T.
  • Journal Title: Nanomedicine Volume: 10 Issue: 3 Pages: 629-637
  • DOI: 10.1016/j.nano.2013.09.007
  • Peer Reviewed
• [Journal Article] Lysyl oxidase like-2 reinforces unsatisfactory ossification induced by bone morphogenetic protein-2: relating nanomechanical properties and molecular changes. (2013)
  • Author(s): Shibata, Y., Suzuki, D., Wurihan, Yamada, A., Maruyama, N., Fujisawa, N., Kamijo, R., Miyazaki, T.
  • Journal Title: Nanomedicine Volume: 9 Issue: 7 Pages: 1036-1047
  • DOI: 10.1016/j.nano.2013.04.008
  • Peer Reviewed
• [Journal Article] BMP-2 induced expression of Alx3 that is a positive regulator of osteoblast differentiation. (2013)
  • Author(s): Matsumoto, T., Yamada, A., Suzuki, D., Aizawa, R., Tsukasaki, M., Yamamoto, M., Baba, K., Kamijo, R.
  • Journal Title: Plos One Volume: 8 Issue: 6 Pages: e68774-e68774
  • DOI: 10.1371/journal.pone.0068774
  • Peer Reviewed
• [Journal Article] Downregulation of carbonic anhydrase IX promotes Col10a1 expression in chondrocytes (2013)
  • Author(s): Maruyama T, Miyamoto Y, Yamamoto G, Yamada A, Yoshimura K, Suzawa T, Takami M, Akiyama T, Hoshino M, Iwasa F, Ikumi N, Tachikawa T, Mishima K, Baba K, Kamijo R
  • Journal Title: PLoS ONE Volume: Vol.8 Issue: 2 Pages: e56984-e56984
  • DOI: 10.1371/journal.pone.0056984
  • Peer Reviewed
• [Journal Article] Synthetic Octacalcium Phosphate Enhanced Reparative Dentin Formation via Induction of Odontoblast Differentiation. (2013)
  • Author(s): X Wang, T Suzawa, T Miyauchi, B Zhao, R Yasuhara, T Anada, N Nakamura, O Suzuki, R Kamijo.
  • Journal Title: J Tissue Eng Regen Med, Volume: in press Issue: 11 Pages: 1310-1320
  • DOI: 10.1002/term.1669
  • Peer Reviewed
• [Journal Article] Octacalcium phosphate suppresses chondrogenic differentiation of ATDC5 cells. (2013)
  • Author(s): I Shibuya, K Yoshimura, Y Miyamoto, A Yamada, M Takami, T Suzawa, D Suzuki, N Ikumi, F Hiura, T Anada, O Suzuki, R Kamijo.
  • Journal Title: Cell Tissue Res Volume: 352 Issue: 2 Pages: 401-412
  • DOI: 10.1007/s00441-012-1548-8
  • Peer Reviewed
• [Journal Article] Cdc42plays important roles in bone formation during limb development. (2013)
  • Author(s): Aizawa, R., Yamada, A., Suzuki, D., Tsukasaki, M., Yamamoto, M., Kamijo, R.
  • Journal Title: Jpn. J. Tissue Cult. Dent. Res. Volume: 22 Pages: 35-41
  • Peer Reviewed
• [Journal Article] The essential roles of small GTPase Rac1 in limb development. (2013)
  • Author(s): Suzuki, D., Yamada, A., Kamijo, R.
  • Journal Title: J. Oral Biosci. Volume: 56 Pages: 116-121
  • Peer Reviewed
• [Journal Article] Rho family small G proteins: lessons from tissue-specific gene knockout studies. (2013)
  • Author(s): Yamada, A., Aiba, A., Kamijo R.
  • Journal Title: J. Oral Biosci. Volume: 56 Pages: 23-29
  • Peer Reviewed
• [Journal Article] 抗RANKL抗体の開発と臨床応用 (2013)
  • Author(s): 高見正道、松永朗裕、稲垣克記、上條竜太郎
  • Journal Title: The Bone Volume: 27 Pages: 71-79
• [Journal Article] Cell adhesion signal regulates RANK expression in osteoclast precursors (2012)
  • Author(s): Mochizuki A, Takami M, Miyamoto Y, Nakamaki T, Tomoyasu S, Kadono Y, Tanaka S, Inoue T, Kamijo R
  • Journal Title: PLoS ONE Volume: 7(11) Issue: 11 Pages: e48795-e48795
  • DOI: 10.1371/journal.pone.0048795
  • Peer Reviewed
• [Journal Article] Antioxidant and osteogenic properties of anodically oxidized titanium (2012)
  • Author(s): Iwai-Yoshida M., Shibata Y., Wurihan, Suzuki D., Fujisawa N., Tanimoto Y., Kamijo R., Maki K. and Miyazaki T.
  • Journal Title: Journal of the Mechanical Behavior of Biomedical Materials Volume: (in press) Pages: 230-236
  • DOI: 10.1016/j.jmbbm.2012.01.016
  • Peer Reviewed
• [Journal Article] Nephronectin expression is regulated by SMAD signaling in osteoblast-like MC3T3-E1 cells (2012)
  • Author(s): Tsukasaki M, Yamada A, Yoshimura K, Miyazono A, Yamamoto M, Takami M, Miyamoto Y, Morimura N, Kamijo R
  • Journal Title: Biochem Biophys Res Commun Volume: 425 Issue: 2 Pages: 390-392
  • DOI: 10.1016/j.bbrc.2012.07.106
  • Peer Reviewed
• [Journal Article] BMP2 differentially regulates the expression of Gremlin1 and Gremlin2, the negative regulators of BMP function, during osteoblast differentiation (2012)
  • Author(s): Suzuki D, Yamada A, Aizawa R, Funato S, Matsumoto T, Suzuki W, Takami M, Miyamoto Y, Suzawa T, Yamamoto M, Baba K, Kamijo R
  • Journal Title: Calcif Tissue Int Volume: 91 Issue: 1 Pages: 88-96
  • DOI: 10.1007/s00223-012-9614-5
  • Peer Reviewed
• [Journal Article] R848, a toll-like receptor 7 agonist, inhibits osteoclast differentiation but not survival or bone-resorbing function of mature osteoclasts (2012)
  • Author(s): Miyamoto A, Takami M, Matsumoto A, Mochizuki A, Yamada T, Tachi K, Shibuya I, Nakamach, T, Shioda S, Baba K, Inoue T, Miyamoto Y, Yim M, Kamijo R
  • Journal Title: Cytotechnology Volume: 64 Issue: 3 Pages: 331-339
  • DOI: 10.1007/s10616-012-9442-5
  • Peer Reviewed
• [Journal Article] Expression of myelin and lymphocyte protein (MAL) in oral carcinogenesis. (2012)
  • Author(s): Pal SK, Noguchi S, Yamamoto G, Yamada A, Isobe T, Hayashi S, Tanaka J, Tanaka Y, Kamijo R, Yamane GY, Tachikawa T
  • Journal Title: Med Mol Morphol Volume: 45 Issue: 4 Pages: 222-228
  • DOI: 10.1007/s00795-011-0563-2
  • NAID: 10031137147
  • Peer Reviewed
• [Journal Article] Mechanisms for bone resorption induced by periodontal pathogens. (2012)
  • Author(s): Yoshimura K, Miyamoto Y, Kamijo R
  • Journal Title: Clin Calcium. Volume: 22 Pages: 1685-1692
• [Journal Article] Nephronectin expression is regulated by SMAD signaling in osteoblast-like MC3T3-E1 cells. (2012)
  • Author(s): ukasaki M, Yamada A, Yoshimura K, Miyazono A, Yamamoto M, Takami M, Miyamoto Y, Morimura N, Kamijo R.
  • Journal Title: Biochem Biophys Res Commun. Volume: 425 Pages: 390-392
  • Peer Reviewed
• [Journal Article] Cdc42 is required for chondrogenesis and interdigital programmed cell death during limb development (2011)
  • Author(s): Aizawa R, Yamada A, Suzuki D, Iimura T, Kassai H, Harada T, Tsukasaki M, Yamamoto G, Tachikawa T, Nakao K, Yamamoto M, Yamagchi A, Aiba A, Kamijo R
  • Journal Title: AMech Dev Volume: (In press) Issue: 1-4 Pages: 38-50
  • DOI: 10.1016/j.mod.2012.02.002
  • Peer Reviewed
• [Journal Article] Shared and distinct functions of the transcription factors IRF4 and IRF8 in myeloid cell development (2011)
  • Author(s): Yamamoto M, et al
  • Journal Title: PLoS ONE Volume: 6 Issue: 10 Pages: e25812-e25812
  • DOI: 10.1371/journal.pone.0025812
  • Peer Reviewed
• [Journal Article] Expression of POEM, a positive regulator of osteoblast differentiation, is suppressed by TNF-α (2011)
  • Author(s): Tsukasaki M, et al
  • Journal Title: Biochem Biophys Res Commun Volume: 410 Issue: 4 Pages: 766-770
  • DOI: 10.1016/j.bbrc.2011.06.048
  • Peer Reviewed
• [Journal Article] Monocarboxylate transporter-1 is required for cell death in mouse chondrocytic ATDC5 cells exposed to interleukin-1β via activation of nuclear factor-κB and expression of phagocyte-type NADPH-oxidase (2011)
  • Author(s): Yoshimura K, et al
  • Journal Title: J Biol Chem Volume: 286 Issue: 17 Pages: 14744-14752
  • DOI: 10.1074/jbc.m111.221259
  • Peer Reviewed
• [Journal Article] Enhancement of bone morphogenetic protein-induced ectopic bone formation by transforming growth factor b (2011)
  • Author(s): Inoue T, Shintani S, Koike T, Honda Y, Suzuki O, Baba K, Kamijo R
  • Journal Title: Tissue Engineering Volume: 17 Issue: 2 Pages: 597-606
  • DOI: 10.1007/s00441-010-1052-y
  • Peer Reviewed
• [Journal Article] Enhancement of bone morphogenetic protein-2-induced ectopic bone formation by transforming growth factor beta1. (2011)
  • Author(s): Tachi K, et al.
  • Journal Title: Tissue Eng Part A Volume: 17 Pages: 597-606
  • Peer Reviewed
• [Journal Article] Monocarboxylate transporter-1 is required for cell death in mouse chondrocytic ATDC5 cells exposed to interleukin-1β via late phase activation of nuclear factor κB and expression of phagocyte-type NADPH oxidase. (2011)
  • Author(s): Yoshimura K, et al.
  • Journal Title: J Biol Chem Volume: 286 Pages: 14744-14752
  • Peer Reviewed
• [Journal Article] Identification and isolation of neural crest derived cells in nasal concha. (2011)
  • Author(s): Ono M, et al.
  • Journal Title: J Tissue Cult Dent Res Volume: 20 Pages: 29-35
  • Peer Reviewed
• [Journal Article] Shared and Distinct Functions of the Transcription Factors IRF4 and IRF8 in Myeloid Cell Development. (2011)
  • Author(s): Tsukasaki M, et al.
  • Journal Title: Biochem Biophys Res Commun Volume: 410 Pages: 766-770
• [Journal Article] Shared and Distinct Functions of the Transcription Factors IRF4 and IRF8 in Myeloid Cell Development. (2011)
  • Author(s): Yamamoto M, et al.
  • Journal Title: PLoS One Volume: 6
• [Journal Article] 破骨細胞分化の制御機構 (2011)
  • Author(s): 高見正道，上條竜太郎
  • Journal Title: 生化学 Volume: 83 Pages: 110-114
  • NAID: 10029067501
• [Presentation] Characteristic gene expression profile of neural crest-derived cells in adult mouse tissues (2013)
  • Author(s): Takahashi M, Suzawa T, Yamada A, Maki K, Kamijo R
  • Organizer: American Society for Cell Biology, 2013 Annual Meeting
  • Place of Presentation: New Orleans, USA
• [Presentation] Monocarboxylate transporter-1は骨芽細胞におけるアルカリホスファターゼの発現に必要である (2013)
  • Author(s): 吉村健太郎,宮本洋一,星野真理江,宮本尚,斎藤章大,船登咲映,泉田恵理,槇宏太郎,山本松男,馬場一美,上條竜太郎
  • Organizer: 日本口腔組織培養学会設立50周年記念学術大会
  • Place of Presentation: 東京
• [Presentation] デキサメタゾンは培養骨芽細胞により形成される石灰化物のリン酸含有量と硬さの向上に必要である (2013)
  • Author(s): 宮本尚,宮本洋一,柴田陽,上條竜太郎,槇宏太郎
  • Organizer: 第72回日本矯正歯科学会大会
  • Place of Presentation: 松本
• [Presentation] 低分子量Gタンパク質Cdc42は軟骨形成に必須の遺伝子である (2013)
  • Author(s): 鈴木航,山田篤,相澤怜,鈴木大,中山睦子,槇宏太郎,山本松男,馬場一美,上條竜太郎
  • Organizer: 第55回歯科基礎医学会学術大会
  • Place of Presentation: 岡山
• [Presentation] Cdc42 is crucial for facial and palatal development (2013)
  • Author(s): Nakayama M, Yamada A, Aizawa R, Isobe T, Suzuki D, Suzuki W, Sato Y, Aiba A, Baba K, Mishima K, Yamamoto M, Maki K, Kamijo R
  • Organizer: 2^<nd> Meeting of the International Association for Dental Research-Asia Pacific Region
  • Place of Presentation: Bangkok, Thailand
• [Presentation] Characteristic gene expression profile of neural crest-derived cells in adult mouse tissues. (2013)
  • Author(s): Takahashi M, Suzawa T, Yamada A, Maki K, Kamijo R
  • Organizer: American Society for Cell Biology 2013 Annual Meeting
  • Place of Presentation: New Orleans, USA
• [Presentation] Glucocorticoids stimulates mouse osteoblastic MC3T3-E1 cells to form apatite-phosphate-containing mineralized nodules in vitro. (2013)
  • Author(s): Miyamoto S, Miyamoto Y, Shibata Y, Miyazaki T, Maki K, Kamijo R
  • Organizer: American Society for Cell Biology 2013 Annual Meeting
  • Place of Presentation: New Orleans, USA
• [Presentation] 成体組織における神経堤由来細胞の遺伝子発現解析 (2013)
  • Author(s): 高橋正晧，須澤徹夫，山田篤、山口徹太郎、上條竜太郎、槇 宏太郎
  • Organizer: 第33回昭和歯学会例会
  • Place of Presentation: 東京
• [Presentation] 骨芽細胞による石灰化物形成におけるグルココルチコイドの役割 (2013)
  • Author(s): 宮本 尚，宮本洋一，柴田 陽，宮﨑 隆，槇 宏太郎，上條竜太郎
  • Organizer: 日本口腔組織培養学会設立50周年記念学術大会
  • Place of Presentation: 東京
• [Presentation] 成体組織に存在する神経堤由来細胞の効率的な分離法を目指した遺伝子発現パターンの解析 (2013)
  • Author(s): 高橋正晧，須澤徹夫，山田 篤、山口徹太郎、上條竜太郎、槇 宏太郎
  • Organizer: 第72回日本矯正歯科学会大会
  • Place of Presentation: 松本
• [Presentation] 骨芽細胞が形成する石灰化物のリン酸カルシウム含有量と硬さはデキサメタゾンにより増強される (2013)
  • Author(s): 宮本 尚、宮本洋一、槇 宏太郎、上條竜太郎
  • Organizer: 日本口腔科学会関東地方部会
  • Place of Presentation: 東京
• [Presentation] 一酸化窒素は8-ニトロ-サイクリックGMPの生成を介して成長板軟骨の伸長を促進する (2013)
  • Author(s): 星野真理江、宮本洋一、馬場一美、上條竜太郎
  • Organizer: 日本口腔科学会関東地方部会
  • Place of Presentation: 東京
• [Presentation] デキサメタゾンは培養骨芽細胞が形成する石灰化物のリン酸含有量と硬さを増強する (2013)
  • Author(s): 宮本 尚、宮本洋一、槇 宏太郎、上條竜太郎
  • Organizer: 第55回歯科基礎医学会学術大会
  • Place of Presentation: 岡山
• [Presentation] Cdc42 is crucial for facial and palatal development. (2013)
  • Author(s): Nakayama M, Yamada A, Aizawa R, Isobe T, Suzuki D, Suzuki W, Sato Y, Aiba A, Baba K, Mishima K, Yamamoto M, Maki K, Kamijo R
  • Organizer: 2nd Meeting of the International Association for Dental Research-Asia Pacific Region
  • Place of Presentation: Bangkok, Thailand
• [Presentation] Nanomechanical Testing Technology for In Vitro Mineralized Tissue. (2013)
  • Author(s): Shibata Y, Suzuki D, Wurihan, Yamada A, Maruyama N, Kamijo R, Miyazaki T
  • Organizer: 2nd Meeting of the International Association for Dental Research-Asia Pacific Region
  • Place of Presentation: Bangkok, Thailand
• [Presentation] 神経堤由来の毛包細胞を用いた骨芽細胞分化誘導 (2013)
  • Author(s): 森澤絵里，高見正道，須澤徹夫，馬場一美，大隅典子，上條竜太郎
  • Organizer: 第67回日本口腔科学会学術集会
  • Place of Presentation: 宇都宮
• [Presentation] 軟骨細胞の増殖・分化における8-ニトロ-環状GMPの役割 (2013)
  • Author(s): 星野真理江，宮本洋一，上條竜太郎，馬場一美
  • Organizer: 日本補綴歯科学会 第122回学術大会
  • Place of Presentation: 福岡
• [Presentation] 歯周病原菌由来リシン特異的ジンジパインによる破骨細胞分化制御 (2013)
  • Author(s): 上條竜太郎
  • Organizer: 日本歯科大学大学院セミナー
  • Place of Presentation: 東京
  • Invited
• [Presentation] 毛包内の神経堤由来細胞による象牙芽細胞分化誘導 (2012)
  • Author(s): 森澤絵里,須澤徹夫,宮内知彦,高見正道,山田篤,宮本洋一,林竜平,西田幸二,大隅典子,馬場一美,上條竜太郎
  • Organizer: 第11回日本再生医療学会総会
  • Place of Presentation: 横浜
• [Presentation] 低分子量Gタンパク質Cdc42は四肢形成における軟骨分化と肢芽指間部の細胞死に必須である (2012)
  • Author(s): 相澤怜,山田篤,鈴木大,飯村忠浩,山本剛,山口朗,山本松男,上條竜太郎
  • Organizer: 第30回日本骨代謝学会学術集会
  • Place of Presentation: 東京
• [Presentation] Monocarboxylate transporter-1はBMP-2刺激後のSmad1のリン酸化に関与することで骨芽細胞分化を正に制御する (2012)
  • Author(s): 吉村健太郎,宮本洋一,星野真理江,秋山智人,塚崎雅之,山田篤,高見正道,須澤徹夫,馬場一美,上條竜太郎
  • Organizer: 第30回日本骨代謝学会学術集会
  • Place of Presentation: 東京
• [Presentation] LPS inhibits ectopic bone formation induced by BMP-2 plus TGF-beta1 in mice (2012)
  • Author(s): Matsumoto A, Takami M, Miyamoto A, Tachi K, Suzawa T, Baba K, Kamijo R
  • Organizer: ASBMR 2012 Annual Meeting
  • Place of Presentation: Minneapolis, MN, USA
• [Presentation] 血管内皮細胞と歯髄細胞の共培養がラット歯髄細胞の石灰化へ与える影響について． (2012)
  • Author(s): 増田宜子，山田嘉重，宮本洋一，上條竜太郎
  • Organizer: 日本歯科保存学会2012年度春季学術大会（第136回）
  • Place of Presentation: 沖縄
• [Presentation] 歯周骨破壊におけるリシン特異的ジンジパインの役割． (2012)
  • Author(s): 秋山智人，星野真理江，上條竜太郎，馬場一美
  • Organizer: 社団法人日本歯科補綴学会第121回学術大会
  • Place of Presentation: 横浜
• [Presentation] 低分子量Gタンパク質Cdc42は四肢形成における軟骨分化と肢芽指間部の細胞死に必須である． (2012)
  • Author(s): 相澤怜，山田篤，鈴木 大，飯村忠浩，山本剛，山口朗，山本松男，上條竜太郎
  • Organizer: 第30回日本骨代謝学会学術集会
  • Place of Presentation: 東京
• [Presentation] Monocarboxylate transporter-1はBMP-2刺激後のSmad1のリン酸化に関与することで骨芽細胞分化を正に制御する． (2012)
  • Author(s): 吉村健太郎，宮本洋一，星野真理江，秋山智人，塚崎雅之，山田 篤，高見正道，須澤徹夫，馬場一美，上條竜太郎
  • Organizer: 第30回日本骨代謝学会学術集会
  • Place of Presentation: 東京
• [Presentation] 低分子量Gタンパク質Cdc42は四肢形成時の軟骨分化および肢芽指間部の細胞死を制御する． (2012)
  • Author(s): 相澤怜, 山田篤, 鈴木大, 飯村忠浩，山本剛，美島健二, 山口朗，山本松男，上條竜太郎
  • Organizer: 第19回BMP研究会
  • Place of Presentation: 東京
• [Presentation] 毛包から採取した神経堤由来細胞による象牙芽細胞分化誘導． (2012)
  • Author(s): 森澤絵里，須澤徹夫，宮内知彦，高橋正皓，槇宏太郎，馬場一美，大隅典子，上條竜太郎
  • Organizer: 第46回日本口腔科学会関東地方部会
  • Place of Presentation: 川越
• [Presentation] 歯周病原菌由来リシン特異的ジンジパインはオステオプロテゲリンを優先的に分解しTNF-alphaおよびIL-1betaによる破骨細胞分化を促進する． (2012)
  • Author(s): 秋山智人，宮本洋一，山田 篤，高見正道，吉村健太郎，星野真理江，宮本 尚，槇 宏太郎，馬場一美，上條竜太郎
  • Organizer: 第54回歯科基礎医学会学術大会・総会
  • Place of Presentation: 郡山
• [Presentation] 成体マウス毛包内の神経堤由来細胞による象牙芽細胞分化誘導． (2012)
  • Author(s): 森澤絵里，須澤徹夫，宮内知彦，鈴木航，馬場一美，上條竜太郎
  • Organizer: 第54回歯科基礎医学会学術大会・総会
  • Place of Presentation: 郡山
• [Presentation] 口腔粘膜上皮のケラチノサイトの一部は神経堤に由来する．第71回日本矯正歯科学会大会プログラム (2012)
  • Author(s): 小野美樹，高見正道，須澤徹夫，綿引淳一，榎本明子，南保友樹，田口智博，市川雄大，野瀬佳奈，上條竜太郎，槇宏太郎
  • Organizer: 第71回日本矯正歯科学会大会
  • Place of Presentation: 盛岡
• [Presentation] Importance of proteolytic degradation of osteoprotegerin by lysine-specific gingipain in periodontal osteoclastogenesis. (2012)
  • Author(s): Akiyama T, Miyamoto Y, Yamada A, Takami M, Yasuhara R, Yoshimura K, Hoshino M, Maruyama T, Mishima K, Baba K, Kamijo R
  • Organizer: ASBMR 2012 Annual Meeting
  • Place of Presentation: Minneapolis, MN, USA
• [Presentation] LPS inhibits ectopic bone formation induced by BMP-2 plus TGF-beta1 in mice. (2012)
  • Author(s): Matsumoto A, Takami M, Miyamoto A, Tachi K, Suzawa T, Baba K, Kamijo R
  • Organizer: ASBMR 2012 Annual Meeting
  • Place of Presentation: Minneapolis, MN, USA
• [Presentation] 低分子量Gタンパク質Cdc42は四肢形成における軟骨分化と肢芽指間部のアポトーシスを制御する． (2012)
  • Author(s): 相澤 怜，山田 篤，鈴木 大，上條竜太郎
  • Organizer: 第49回日本口腔組織培養学会学術集会
  • Place of Presentation: 広島
• [Presentation] Cdc42の軟骨形成における機能解析． (2012)
  • Author(s): 鈴木 航，山田 篤，相澤 怜，鈴木 大，中山睦子，槇 宏太郎，山本松男，上條竜太郎，馬場一美
  • Organizer: 第32回昭和歯学会例会
  • Place of Presentation: 東京
• [Presentation] Small GTPase Cdc42 is essential for cartilage development. (2012)
  • Author(s): Suzuki W, Yamada a, Suzuki D, Aizawa R,Nakayama M, Maki K, Yamamoto M, Takeda S, Baba K, Kamijo R.
  • Organizer: 第60回国際歯科研究学会日本部会（JADR）総会・学術大会
  • Place of Presentation: 新潟
• [Presentation] 歯周病原菌由来の破骨細胞分化制御因子：リシン特異的ジンジパイン (2012)
  • Author(s): 上條竜太郎
  • Organizer: 北海道大学歯学研究セミナー
  • Place of Presentation: 札幌
  • Invited
• [Presentation] 毛包から純化した神経堤細胞による象牙芽細胞への分化誘導 (2011)
  • Author(s): 宮内知彦,須澤徹夫,小野美樹,林竜平,西田幸二,槇宏太郎,上條竜太郎,馬場一美
  • Organizer: 日本歯科補綴学会第120回記念学術大会
  • Place of Presentation: 広島
• [Presentation] Dual effects of lysine-specific gingipain on inflammatory osteoclastogenesis (2011)
  • Author(s): Akiyama T, Miyamoto Y, Yamada A, Takami M, Yoshimura K, Yasuhara R, Tachikawa T, Baba K, Kamijo R
  • Organizer: The Joint International Meeting Sponsored by The Jpn. Soc. for Interferon and Cytokine Research
  • Place of Presentation: Osaka
• [Presentation] Monocarboxylate transporter-1 is required for cell death in mouse chondrocyte-kike ATDC5 cells exposed to interleukin-1β; via late phase activation of NF-κB and expression of phagocyte-type NADPH-oxidase (2011)
  • Author(s): Yoshimura K, Miyamoto Y, Yasuhara R, Maruyama T, Akiyama T, Yamada A, Takami M, Suzawa T, Tsunawaki S, Tachikawa T, Baba K, Kamijo R
  • Organizer: The Joint International Meeting Sponsored by The Jpn. Soc. for Interferon and Cytokine Research
  • Place of Presentation: Osaka
• [Presentation] 二本鎖RNAアナログはインターフェロンβ/STAT1経路を介して破骨細胞分化を抑制し骨粗鬆症モデル動物の骨量を増加させる (2011)
  • Author(s): 宮本阿礼,高見正道,宮本洋一,山田篤,望月文子,井上富雄,上條竜太郎
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
• [Presentation] Monocarboxylate transporter-1は骨芽細胞分化を正に制御する (2011)
  • Author(s): 吉村健太郎,宮本洋一,塚崎雅之,山田篤,秋山智人,馬場一美,上條竜太郎
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
• [Presentation] 神経堤由来細胞を用いた顎顔面再建方法確立のための基礎的研究 (2011)
  • Author(s): 小野美樹,高見正道,須澤徹夫,山田篤,宮内知彦,馬場一美,中村雅典,槇宏太郎,上條竜太郎
  • Organizer: 第70回東京矯正歯科学会記念大会
  • Place of Presentation: 東京
• [Presentation] Double stranded RNA increases bone mass in osteoporosis model mice by inhibiting osteoclastogenesis via interferon-β/STAT1 pathway (2011)
  • Author(s): Miyamoto A, Takami M, Miyamoto Y, Yamada A, Yim M, Tamura T, Kamijo R
  • Organizer: IOF-ANZBMR
  • Place of Presentation: Gold Coast, Australia
• [Presentation] Cdc42は四肢形成における軟骨形成と肢芽指間域のアポトーシスを制御する (2011)
  • Author(s): 相澤怜,山田篤,鈴木大,塚崎雅之,山本剛,飯村忠浩,山口朗,山本松男,上條竜太郎
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 岐阜
• [Presentation] 毛包から純化した神経堤細胞による象牙芽細胞への分化誘導． (2011)
  • Author(s): 宮内知彦，他
  • Organizer: 日本歯科補綴学会 第120回記念学術大会
  • Place of Presentation: 広島
• [Presentation] Dual effects of lysine-specific gingipain on inflammatory osteoclastogenesis. (2011)
  • Author(s): Akiyama T, et al.
  • Organizer: The Joint International Meeting Sponsored by The Jpn. Soc. for Interferon and Cytokine Research,
  • Place of Presentation: Osaka
• [Presentation] Monocarboxylate transporter-1 is required for cell death in mouse chondrocyte-kike ATDC5 cells exposed to interleukin-1&#61538; via late phase activation of NF-&#61547;B and expression of phagocyte-type NADPH-oxidase. (2011)
  • Author(s): Yoshimura K, et al.
  • Organizer: The Joint International Meeting Sponsored by The Jpn. Soc. for Interferon and Cytokine Research,
  • Place of Presentation: Osaka
• [Presentation] 神経堤由来細胞を用いた顎顔面再建方法確立のための基礎的研究． (2011)
  • Author(s): 小野美樹，他
  • Organizer: 第70回東京矯正歯科学会記念大会
  • Place of Presentation: 東京
• [Presentation] TNF-alphaは細胞接着分子POEMの発現を抑制し、骨芽細胞分化を制御している. (2011)
  • Author(s): 塚崎雅之，他
  • Organizer: 第31回昭和歯学会総会
  • Place of Presentation: 東京
• [Presentation] 二本鎖RNAアナログはインターフェロンβ/STAT1経路を介して破骨細胞分化を抑制し骨粗鬆症モデル動物の骨量を増加させる． (2011)
  • Author(s): 宮本阿礼，他
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
• [Presentation] 成体における神経堤由来細胞の分布とそれを用いた骨芽細胞の分化誘導． (2011)
  • Author(s): 小野美樹，他
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
• [Presentation] リシン特異的ジンジパインによる炎症性骨破壊促進機構の解析． (2011)
  • Author(s): 秋山智人，他
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
• [Presentation] Monocarboxylate transporter-1は骨芽細胞分化を正に制御する． (2011)
  • Author(s): 吉村健太郎，他
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
• [Presentation] 関節表層細胞の維持と機能調節におけるWnt/&#61538;-cateninとRac1シグナルのクロストーク. (2011)
  • Author(s): 安原理佳，他
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
• [Presentation] TNF-alphaは骨芽細胞においてPOEMの発現を抑制し、骨芽細胞分化を制御する. (2011)
  • Author(s): 塚崎雅之，他
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
• [Presentation] Double stranded RNA increases bone mass in osteoporosis model mice by inhibiting osteoclastogenesis via interferon-&#61538;/STAT1 pathway. (2011)
  • Author(s): Miyamoto A, et al.
  • Organizer: IOF-ANZBMR
  • Place of Presentation: Gold Coast, Australia
• [Presentation] Cdc42は四肢形成における軟骨形成と肢芽指間域のアポトーシスを制御する． (2011)
  • Author(s): 相澤 怜，他
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 岐阜
• [Presentation] 口腔および食道上皮を構成するケラチノサイトの一部は神経堤に由来する． (2011)
  • Author(s): 小野美樹，他
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 岐阜
• [Presentation] TNF-alphaはPOEMの発現を抑制し、骨芽細胞分化を制御する. (2011)
  • Author(s): 塚崎雅之，他
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 岐阜
• [Presentation] TGF-betaによるエナメル芽細胞分化におけるCaspase-14の役割． (2011)
  • Author(s): 宮園あがさ，他
  • Organizer: 第53回歯科基礎医学会学術大会
  • Place of Presentation: 岐阜
• [Presentation] 血管内皮細胞がラット歯髄細胞へ与える影響について． (2011)
  • Author(s): 増田宜子，他
  • Organizer: 日本歯科保存学会2011年度秋季学術大会（第135回）
  • Place of Presentation: 大阪
• [Presentation] 口腔・顎顔面部に存在する神経堤由来細胞を用いた硬組織再生法の確立． (2011)
  • Author(s): 須澤徹夫，他
  • Organizer: 第11回東京骨関節フォーラム
  • Place of Presentation: 東京
• [Presentation] Cdc42は四肢形成において軟骨形成と指間部アポトーシスに必須である． (2011)
  • Author(s): 相澤 怜，他
  • Organizer: 第31回昭和歯学会総会
  • Place of Presentation: 東京
• [Book] アインチエイジングシリーズ3骨研究最前線 (2013)
  • Author(s): 吉村健太郎,宮本洋一,上條竜太郎
  • Publisher: 株式会社エヌ・ティーエス
• [Book] アインチエイジングシリーズ３骨研究最前線 (2013)
  • Author(s): 吉村健太郎，宮本洋一，上條竜太郎
  • Total Pages: 428
  • Publisher: 株式会社エヌ・ティーエス
• [Book] The New Frontiers in Research for Oral Cancer (2012)
  • Author(s): Takami M, Miyamoto A, Matsumoto A, Mochizuki A, Tachi K, Baba K, Inoue T, Mijung Y, Shibuya I, Zhao B, Kamijo R
  • Publisher: Maruzen Planet
• [Book] The New Frontiers in Research for Oral Cancer (2012)
  • Author(s): Takami M, Miyamoto A, Matsumoto A, Mochizuki A, Tachi K, Baba K, Inoue T, Mijung Y, Shibuya I, Zhao B, Kamijo K.
  • Total Pages: 209
  • Publisher: Maruzen Planet
• [Book] 骨の再生医学・再生医療～効果的骨再生をめざして～.臨床家のための矯正イヤーブック (2011)
  • Author(s): 上條竜太郎
  • Total Pages: 11
  • Publisher: クインテッセンス出版
• [Book] 骨の再生医学・再生医療～効果的骨再生をめざして～．臨床家のための矯正イヤーブック’11 (2011)
  • Author(s): 上條竜太郎
  • Total Pages: 5
  • Publisher: クインテッセンス出版
• [Remarks] 昭和大学歯学部口腔生化学講座ホームページ
  • URL: http://www10.showa-u.ac.jp/~oralbio/