| Project/Area Number |
23592755
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Osaka Dental University |
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Principal Investigator |
IKEO Takashi 大阪歯科大学, 歯学部, 教授 (40159603)
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| Co-Investigator(Kenkyū-buntansha) |
GODA Seiji 大阪歯科大学, 歯学部, 講師 (70351476)
TAKEUCHI Osamu 大阪歯科大学, 歯学部, 助教 (70548320)
KAMADA Aiko 大阪歯科大学, 歯学部, 准教授 (50140215)
YOSHIKAWA Yoshihiro 大阪歯科大学, 歯学部, 歯学部 (70434793)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | う蝕 / 象牙質 / MMP |
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| Research Abstract |
In this project, we examined the molecular mechanism of caries progression caused by dentin pulp complex derived MMPs. First, we examined the secretion of MMP-1 and MMP-3 from cultured dental pulp fibroblast. Pro-inflammatory cytokine TNFa increased secretion of the MMPs. We found JNK inhibitor SP601245 suppressed TNFa induced MMP-3 secretion. Next, to know the key transcription factor for the MMP-3 production in response to TNFa, we had screened possible transcription factors and found CREB as a candidate. To elucidate whether the activity of CREB is controlled by JNK1/2, we conducted pharmacological and genetic suppression of JNK1/2 and found that suppression of JNK 1/2 resulted in deactivation of CREB.
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