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Isolation of thecandidate gene for the marker of the body time determinant in human saliva.

Research Project

Project/Area Number 23592756
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Functional basic dentistry
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

ONISHI Yoshiaki  独立行政法人産業技術総合研究所, バイオメディカル研究部門, 研究グループ長 (60233219)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords口腔生化学 / 唾液 / 転写 / クロマチン / 生物時計
Research Abstract

We have identified 22 genes which are controlled directly by clock genes and show circadian transcription by the combination of ChIP on Chip and microarray experiments using human salivary gland cell line HSG. Rev-erb alpha was included in the 22 genes as a positive control. Then, 9 genes including Rev-erb alpha were selected as genes expressing in the normal human salivary gland by RT-PCR. In these 9 genes we found that 3 genes except Rev-erb alpha showed clear circadian transcription. Finally we have succeeded to identify the gene whose product is detected in saliva by western blot analysis. These results suggest that the selected gene is the good candidate for the marker of the body time determinant in human saliva.

Report

(5 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Products Report
  • Research Products

    (22 results)

All 2014 2013 2012 2011 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (13 results) Patent(Industrial Property Rights) (4 results) (of which Overseas: 3 results)

  • [Journal Article] Shikonin shortens the circadian period: possible involvement of Top2 inhibition.2014

    • Author(s)
      Ogawa Y, Kawano Y, Yamazaki Y, Onishi Y.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 442 Issue: 1 Pages: 339-343

    • DOI

      10.1016/j.bbrc.2013.11.116

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] DNA methylation of the BMAL1 promoter.2013

    • Author(s)
      Satou R, Sugihara N, Ishizuka Y, Matsukubo T, Onishi Y.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 440 Issue: 3 Pages: 449-453

    • DOI

      10.1016/j.bbrc.2013.09.124

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rhythmic binding of Topoisomerase I impacts on the transcription of Bmal1 and circadian period2012

    • Author(s)
      Onishi Y, Kawano Y
    • Journal Title

      Nucleic Acids Res

      Volume: 40 Issue: 19 Pages: 9482-9492

    • DOI

      10.1093/nar/gks779

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Lycorine, a candidate for the control of period length in mammalian cells2012

    • Author(s)
      Onishi Y, Kawano Y, Yamazaki Y
    • Journal Title

      Cell Physiol Biochem

      Volume: 29 Issue: 3-4 Pages: 407-416

    • DOI

      10.1159/000338495

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] The harmala alkaloid harmine is a modulator of circadian Bmal1 transcription2012

    • Author(s)
      Onishi Y, Oishi K, Kawano Y, Yamazaki Y
    • Journal Title

      Biosci Rep

      Volume: 32 Issue: 1 Pages: 45-52

    • DOI

      10.1042/bsr20110002

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] DNA methylation of the BMAL1 promoter2013

    • Author(s)
      佐藤涼一、杉原直樹、石塚洋一、松久保隆、大西芳秋
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2013-12-04
    • Related Report
      2013 Final Research Report
  • [Presentation] 時計遺伝子BMAL1プロモーター領域のDNAメチル化2013

    • Author(s)
      佐藤涼一、杉原直樹、石塚洋一、松久保隆、大西芳秋
    • Organizer
      第296回東京歯科大学学会
    • Place of Presentation
      東京
    • Year and Date
      2013-10-19
    • Related Report
      2013 Final Research Report
  • [Presentation] Rhythmic binding of Topoisomerase I impacts on the transcription of Bmal1 and circadian period2013

    • Author(s)
      大西芳秋、河野泰広
    • Organizer
      日本組織培養学会第86回大会
    • Place of Presentation
      つくば
    • Year and Date
      2013-05-30
    • Related Report
      2013 Final Research Report
  • [Presentation] Regulation of adipocytokines by natural product in cultured 3T3-L1 and RAW264 cells2013

    • Author(s)
      河野泰広、大西芳秋、山崎幸苗
    • Organizer
      日本組織培養学会第86回大会
    • Place of Presentation
      つくば
    • Year and Date
      2013-05-30
    • Related Report
      2013 Final Research Report
  • [Presentation] Rhythmic binding of topoisomerase I impacts on the transcription of Bmal1 and circadian period.2013

    • Author(s)
      大西芳秋、河野泰広
    • Organizer
      日本組織培養学会第86回
    • Place of Presentation
      つくば
    • Related Report
      2013 Annual Research Report
  • [Presentation] Regulation of adipocytokines by natural product in cultured 3T3-L1 and RAW264 cells.2013

    • Author(s)
      河野泰広、大西芳秋、山崎幸苗
    • Organizer
      日本組織培養学会第86回
    • Place of Presentation
      つくば
    • Related Report
      2013 Annual Research Report
  • [Presentation] 時計遺伝子BMAL1プロモーター領域のDNAメチル化2013

    • Author(s)
      佐藤涼一、杉原直樹、石塚洋一、松久保隆、大西芳秋
    • Organizer
      第296回東京歯科大学学会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] DNA methylation of the BMAL1 promoter.2013

    • Author(s)
      佐藤涼一、杉原直樹、石塚洋一、松久保隆、大西芳秋
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] Rhythmic binding of Topoisomerase I impacts on the transcription of Bmal1 and circadian period2012

    • Author(s)
      大西芳秋、河野泰広
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-14
    • Related Report
      2013 Final Research Report
  • [Presentation] Epigenetic regulation of clock genes during the differentiation of embryonic stem (ES) cells2011

    • Author(s)
      大西芳秋、奥田晶彦、西本正純
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Lycorine, a modulator of circadian Bmal1 transcription2011

    • Author(s)
      大西芳秋、河野泰広、山崎幸苗
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2011-09-22
    • Related Report
      2013 Final Research Report
  • [Presentation] Lycorine, a modulator of circadian Bmal1 transcription.2011

    • Author(s)
      大西芳秋、河野泰広、山崎幸苗
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館(京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] Rhythmic binding of Topoisomerase I impacts on the transcription of Bmal1 and circadian period

    • Author(s)
      Onishi Y., Kawano Y.
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡市(福岡国際会議場・マリンメッセ福岡)
    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] 生体リズムマーカー2013

    • Inventor(s)
      大西芳秋
    • Industrial Property Rights Holder
      大西芳秋
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-095888
    • Filing Date
      2013-04-30
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 生体リズムの制御剤2013

    • Inventor(s)
      大西芳秋、大石勝隆
    • Industrial Property Rights Holder
      大西芳秋、大石勝隆
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-029057
    • Filing Date
      2013-02-18
    • Related Report
      2013 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 生体リズムマーカー2013

    • Inventor(s)
      大西 芳秋
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-095888
    • Filing Date
      2013-04-30
    • Acquisition Date
      2017-08-10
    • Related Report
      Products Report
  • [Patent(Industrial Property Rights)] 生体リズムの制御剤2012

    • Inventor(s)
      大西芳秋、山崎幸苗、河野泰広、丸 山進、大石勝隆
    • Industrial Property Rights Holder
      大西芳秋、山崎幸苗、河野泰広、丸 山進、大石勝隆
    • Industrial Property Rights Type
      特許
    • Acquisition Date
      2012-05-24
    • Related Report
      2013 Final Research Report
    • Overseas

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Published: 2011-08-05   Modified: 2019-07-29  

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