Isolation of thecandidate gene for the marker of the body time determinant in human saliva.

• Project/Area Number: 23592756
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: ONISHI Yoshiaki 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 研究グループ長 (60233219)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 口腔生化学 / 唾液 / 転写 / クロマチン / 生物時計

Research Abstract

We have identified 22 genes which are controlled directly by clock genes and show circadian transcription by the combination of ChIP on Chip and microarray experiments using human salivary gland cell line HSG. Rev-erb alpha was included in the 22 genes as a positive control. Then, 9 genes including Rev-erb alpha were selected as genes expressing in the normal human salivary gland by RT-PCR. In these 9 genes we found that 3 genes except Rev-erb alpha showed clear circadian transcription. Finally we have succeeded to identify the gene whose product is detected in saliva by western blot analysis. These results suggest that the selected gene is the good candidate for the marker of the body time determinant in human saliva.

Research Products

• [Journal Article] Shikonin shortens the circadian period: possible involvement of Top2 inhibition. (2014)
  • Author(s): Ogawa Y, Kawano Y, Yamazaki Y, Onishi Y.
  • Journal Title: Biochem Biophys Res Commun. Volume: 442 Issue: 1 Pages: 339-343
  • DOI: 10.1016/j.bbrc.2013.11.116
  • Peer Reviewed
• [Journal Article] DNA methylation of the BMAL1 promoter. (2013)
  • Author(s): Satou R, Sugihara N, Ishizuka Y, Matsukubo T, Onishi Y.
  • Journal Title: Biochem Biophys Res Commun Volume: 440 Issue: 3 Pages: 449-453
  • DOI: 10.1016/j.bbrc.2013.09.124
  • Peer Reviewed
• [Journal Article] Rhythmic binding of Topoisomerase I impacts on the transcription of Bmal1 and circadian period (2012)
  • Author(s): Onishi Y, Kawano Y
  • Journal Title: Nucleic Acids Res Volume: 40 Issue: 19 Pages: 9482-9492
  • DOI: 10.1093/nar/gks779
  • Peer Reviewed
• [Journal Article] Lycorine, a candidate for the control of period length in mammalian cells (2012)
  • Author(s): Onishi Y, Kawano Y, Yamazaki Y
  • Journal Title: Cell Physiol Biochem Volume: 29 Issue: 3-4 Pages: 407-416
  • DOI: 10.1159/000338495
  • Peer Reviewed
• [Journal Article] The harmala alkaloid harmine is a modulator of circadian Bmal1 transcription (2012)
  • Author(s): Onishi Y, Oishi K, Kawano Y, Yamazaki Y
  • Journal Title: Biosci Rep Volume: 32 Issue: 1 Pages: 45-52
  • DOI: 10.1042/bsr20110002
  • Peer Reviewed
• [Presentation] DNA methylation of the BMAL1 promoter (2013)
  • Author(s): 佐藤涼一、杉原直樹、石塚洋一、松久保隆、大西芳秋
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2013-12-04
• [Presentation] 時計遺伝子BMAL1プロモーター領域のDNAメチル化 (2013)
  • Author(s): 佐藤涼一、杉原直樹、石塚洋一、松久保隆、大西芳秋
  • Organizer: 第296回東京歯科大学学会
  • Place of Presentation: 東京
  • Year and Date: 2013-10-19
• [Presentation] Rhythmic binding of Topoisomerase I impacts on the transcription of Bmal1 and circadian period (2013)
  • Author(s): 大西芳秋、河野泰広
  • Organizer: 日本組織培養学会第86回大会
  • Place of Presentation: つくば
  • Year and Date: 2013-05-30
• [Presentation] Regulation of adipocytokines by natural product in cultured 3T3-L1 and RAW264 cells (2013)
  • Author(s): 河野泰広、大西芳秋、山崎幸苗
  • Organizer: 日本組織培養学会第86回大会
  • Place of Presentation: つくば
  • Year and Date: 2013-05-30
• [Presentation] Rhythmic binding of topoisomerase I impacts on the transcription of Bmal1 and circadian period. (2013)
  • Author(s): 大西芳秋、河野泰広
  • Organizer: 日本組織培養学会第８６回
  • Place of Presentation: つくば
• [Presentation] Regulation of adipocytokines by natural product in cultured 3T3-L1 and RAW264 cells. (2013)
  • Author(s): 河野泰広、大西芳秋、山崎幸苗
  • Organizer: 日本組織培養学会第８６回
  • Place of Presentation: つくば
• [Presentation] 時計遺伝子BMAL1プロモーター領域のDNAメチル化 (2013)
  • Author(s): 佐藤涼一、杉原直樹、石塚洋一、松久保隆、大西芳秋
  • Organizer: 第２９６回東京歯科大学学会
  • Place of Presentation: 東京
• [Presentation] DNA methylation of the BMAL1 promoter. (2013)
  • Author(s): 佐藤涼一、杉原直樹、石塚洋一、松久保隆、大西芳秋
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] Rhythmic binding of Topoisomerase I impacts on the transcription of Bmal1 and circadian period (2012)
  • Author(s): 大西芳秋、河野泰広
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-14
• [Presentation] Epigenetic regulation of clock genes during the differentiation of embryonic stem (ES) cells (2011)
  • Author(s): 大西芳秋、奥田晶彦、西本正純
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] Lycorine, a modulator of circadian Bmal1 transcription (2011)
  • Author(s): 大西芳秋、河野泰広、山崎幸苗
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-22
• [Presentation] Lycorine, a modulator of circadian Bmal1 transcription. (2011)
  • Author(s): 大西芳秋、河野泰広、山崎幸苗
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館（京都）
• [Presentation] Rhythmic binding of Topoisomerase I impacts on the transcription of Bmal1 and circadian period
  • Author(s): Onishi Y., Kawano Y.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡市（福岡国際会議場・マリンメッセ福岡）
• [Patent(Industrial Property Rights)] 生体リズムマーカー (2013)
  • Inventor(s): 大西芳秋
  • Industrial Property Rights Holder: 大西芳秋
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-095888
  • Filing Date: 2013-04-30
  • Overseas
• [Patent(Industrial Property Rights)] 生体リズムの制御剤 (2013)
  • Inventor(s): 大西芳秋、大石勝隆
  • Industrial Property Rights Holder: 大西芳秋、大石勝隆
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-029057
  • Filing Date: 2013-02-18
  • Overseas
• [Patent(Industrial Property Rights)] 生体リズムマーカー (2013)
  • Inventor(s): 大西 芳秋
  • Industrial Property Rights Holder: 産業技術総合研究所
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2013-095888
  • Filing Date: 2013-04-30
  • Acquisition Date: 2017-08-10
• [Patent(Industrial Property Rights)] 生体リズムの制御剤 (2012)
  • Inventor(s): 大西芳秋、山崎幸苗、河野泰広、丸 山進、大石勝隆
  • Industrial Property Rights Holder: 大西芳秋、山崎幸苗、河野泰広、丸 山進、大石勝隆
  • Industrial Property Rights Type: 特許
  • Acquisition Date: 2012-05-24
  • Overseas