The roles of HIF-DEC pathway on DNA repair systems in hypoxic oral cancer cells.
Project/Area Number |
23592766
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Pathobiological dentistry/Dental radiology
|
Research Institution | Hiroshima University |
Principal Investigator |
TANIMOTO Keiji 広島大学, 原爆放射線医科学研究所, 助教 (90335688)
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | Hypoxia / HIF / DEC / DNA repair / Transcription / Cancer cells / Cancer cell |
Research Abstract |
In this study, we analyzed expression changes of numerous DNA repair-related genes under hypoxia, further noted that the effect of the changes to radiation sensitivities. Comprehensive gene expression analyses showed that 50 of 130 DNA repair-related genes were included in down-regulated genes under hypoxia. Among these genes we selected 6 genes and confirmed quantitative decreased expressions. Co-transfection reporter assay elucidated that DEC1 or DEC2 repressed those DNA repair-related genes promoter activities. In addition, knockdown of HIF1A or DEC2 attenuated hypoxic suppression of DNA repair-related gene expressions. Interestingly, detection and repair of DNA double-strand breaks by irradiations were inhibited in hypoxic pre-incubated cells. Moreover, knockdown of DEC2 increased radiation sensitivities in those cells. These data suggested that HIF-1-DEC pathway especially DEC2 play a pivotal role in radiation sensitivity via suppression of DNA repair gene expressions.
|
Report
(4 results)
Research Products
(28 results)
-
-
-
[Journal Article] The Krüppel-like zinc finger transcription factor, GLI-similar 1, is regulated by hypoxia-inducible factors via non-canonical mechanisms2013
Author(s)
Elham Khalesi, Hideaki Nakamura, Kian Leong Lee, Andika Chandra Putra, Takahiro Fukazawa, Yumi Kawahara, Yuichi Makino, Lorenz Poellinger, Louis Yuge, Keiji Tanimoto
-
Journal Title
Biochemical and Biophysical Research Communications
Volume: 441
Pages: 499-506
Related Report
Peer Reviewed
-
-
[Journal Article] Interactive effects of cell therapy and rehabilitation realize the full potential of neurogenesis in brain injury model2013
Author(s)
Takeshi Imura, Masaya Matsumoto, Takahiro Fukazawa, Elham Khalesi, Ya-Nan Sun, Masaaki Takeda, Hiroyuki Uwatoko, Kyosuke Nakata, Keiji Tanimoto, Teruyuki Kajiume, Yumi Kawahara, Louis Yuge
-
Journal Title
Neuroscience Letters
Volume: 555
Pages: 73-78
Related Report
Peer Reviewed
-
-
[Journal Article] Development of lymphoproliferative diseases by hypoxia-inducible factor-1αis associated with prolonged lymphocyte survival2013
Author(s)
Eisaburo Sueoka, Naoko Sueoka-Aragane, Akemi Sato, Masaru Ide, Hideaki Nakamura, Yusuke Sotomaru, Choji Taya, Hiromichi Yonekawa, Tomoyuki Kitagawa, Yasushi Kubota, Shinya Kimura, Kei Nakachi, Keiji Tanimoto
-
Journal Title
Related Report
Peer Reviewed
-
-
[Journal Article] The Krüppel-like zinc finger transcription factor, GLI-similar 1, is regulated by hypoxia-inducible factors via non-canonical mechanisms.2013
Author(s)
Khalesi E, Nakamura H, Leong Lee K, Chandra Putra A, Fukazawa T, Kawahara Y, Makino Y, Poellinger L, Yuge L, Tanimoto K.
-
Journal Title
Biochem Biophys Res Commun
Volume: 441
Issue: 2
Pages: 499-506
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] Interactive effects of cell therapy and rehabilitation realize the full potential of neurogenesis in brain injury model2013
Author(s)
Imura T., Matsumoto M., Fukazawa T., Khalesi E., Sun Y.N., Takeda M., Uwatoko H., Nakata K., Tanimoto K., Kajiume T., Kawahara Y., Yuge L
-
Journal Title
Neurosci Lett
Volume: 555
Pages: 73-78
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
[Journal Article] TMEM158 and FBLP1 as Novel Marker Genes of Cisplatin Sensitivity in Non-small Cell Lung Cancer Cells2012
Author(s)
Ahmed El Sayed Mohammed, Hidetaka Eguchi, Satoru Wada, Nobuyuki Koyama, Michio Shimizu, Keiko Otani, Megu Ohtaki, Keiji Tanimoto, Keiko Hiyama, Mohammed Soliman Gaber, Masahiko Nishiyama
-
Journal Title
Experimental Lung Research
Volume: 38
Pages: 463-74
Related Report
Peer Reviewed
-
-
[Journal Article] IL-1β-mediated up-regulation of DEC1 in human gingiva cells via the Akt pathway2012
Author(s)
Ujjal K. Bhawal, Yumi Ito, Keiji Tanimoto, Fuyuki Sato, Katsumi Fujimoto, Takeshi Kawamoto, Tomonori Sasahira, Nobushiro Hamada, Hiroki Kuniyasu, Hirohisa Arakawa, Yukio Kato, Yoshimitsu Abiko
-
Journal Title
Journal of Cellular Biochemistry
Volume: 113
Pages: 3246-3253
Related Report
Peer Reviewed
-
-
[Journal Article] Basic helix-loop-helix transcription factor DEC1 negatively regulates CyclinD12011
Author(s)
Ujjal K. Bhawal, Fuyuki Sato, Yuki Arakawa, Katsumi Fujimoto, Takeshi Kawamoto, Keiji Tanimoto, Yumi Ito, Tomonori Sasahira, Takashi Sakurai, Masaru Kobayashi, Isamu Kashima, Hiroshi Kijima, Hiroki Kuniyasu, Yoshimitsu Abiko, Yukio Kato, Sadao Sato
-
Journal Title
Journal of Pathology
Volume: 224
Pages: 420-429
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
[Presentation] DNA修復関連遺伝子の低酸素応答性転写抑制機構2011
Author(s)
谷本圭司,中村秀明,河本健,加藤幸夫,檜山英三,檜山桂子,西山正彦,森田明典,細井義夫
Organizer
第54回放射線影響学会ワークショップ低酸素バイオロジー研究に基づく放射線生物学・放射線腫瘍学の新展開
Place of Presentation
神戸
Year and Date
2011-11-19
Related Report
-
-
[Presentation] がん細胞におけるVHL蛋白制御機構2011
Author(s)
谷本圭司,江口英孝,和田智,西山正彦,檜山英三,中村秀明,Lorenz Poellinger,檜山桂子
Organizer
第8回がんとハイポキシア研究会
Place of Presentation
札幌
Year and Date
2011-01-30
Related Report