Are SK-N-SH cells after space radiation exposure (space cells) radioresistance or not?

• Project/Area Number: 23592774
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Kagoshima University
• Principal Investigator: INDO HIROKO 鹿児島大学, 医歯(薬)学総合研究科, 助教 (00301391)
• Co-Investigator(Kenkyū-buntansha): MAJIMA Hideyuki J. 鹿児島大学, 医歯学総合研究科, 教授 (60165701) ; 富田 和男 鹿児島大学, 医歯学総合研究科, 助教 (60347094)
• Co-Investigator(Renkei-kenkyūsha): TOMITA Kazuo 鹿児島大学, 医歯学総合研究科, 助教 (60347094)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: radioresistance / cell death / autophagy / MnSOD / オートファジー

Outline of Final Research Achievements

Radiation exposure dose in space accounts for 0.2 ~ 10 mSv per day. We previously suggested that low dose radiation as well as microgravity may cause in intracellular oxidative stress and result in different types of cell death. We send the SK-N-SH cells to International Space Station, at Japan Experimental Module, in 2010, and cells were cultured in JEM. Cells were frozen and came back to ground. After that, cells were thawed up and have been re-cultured (space cells).
We examined whether space cells are radioresistance or not? Space cells were generated more intracellular ROS and MnSOD activity. Space cells after 10Gy X-arradiation induced survivin expression and reduced Beclin1,resulting in inhibit autophagy. On the other hands, MnSOD overexpessed space cells after 10Gy x-irradiation induced Bcl-2 expression compared with control cells. The results suggest that SK-N-SH cells after space radiation exposure (space cells) are radioresistance on X-ray induce cell death.

Research Products

• [Journal Article] A mitochondrial superoxide theory for oxidative stress diseases and aging (2015)
  • Author(s): Indo HP, Yen H-C, Nakanishi I, Matsumoto K, Tamura M, Nagano Y, Matsui H, Gusev RO, Cornette R, Okuda T, Minamiyama Y, Ichikawa H, Suenaga S, Oki M, Sato T, Ozawa T, St, Clair DK, Majima HJ
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 56 Issue: 1 Pages: 1-7
  • DOI: 10.3164/jcbn.14-42
  • NAID: 130004879365
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed / Open Access
• [Journal Article] MnSOD downregulation induced by extremely low 0.1 mGy single and fractionated X-rays and microgravity treatment in human neuroblastoma cell line, NB-1 (2015)
  • Author(s): Indo HP, Tomiyoshi T, Suenaga, S, Tomita K, Suzuki H, Masuda D, Terada M, Ishioka N, Gusev O, Cornette R, Okuda T, Mukai C and Majima HJ.
  • Journal Title: J Clin Biochem Nurt. Volume: 印刷中
  • NAID: 130005096226
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Mitochondrial reactive oxygen species accelerate gastric cancer cell invasion (2014)
  • Author(s): Masato Tamura, Hirofumi Matsui, Tsutomu Tomita, Hisato Sadakata, Hiroko P. Indo, Hideyuki J. Majima, Tsuyoshi Kaneko, Ichinosuke Hyodo.
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 54 Issue: 1 Pages: 12-17
  • DOI: 10.3164/jcbn.13-36
  • NAID: 130004466736
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed
• [Journal Article] Reactive oxygen species involved cancer cellular specific 5-aminolevulinic acid uptake in gastric epithelial cells (2014)
  • Author(s): Ito H, Tamura M, Matsui H, Majima HJ, Indo HP, Hyodo I.
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 54 Issue: 2 Pages: 81-85
  • DOI: 10.3164/jcbn.13-98
  • NAID: 130004879350
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed
• [Journal Article] Blue LED light exposure develops intracellular reactive oxygen species, lipid peroxidation, and subsequent cellular injuries in cultured bovine retinal pigment epithelial cells. (2013)
  • Author(s): Nakanishi-Ueda T, Majima HJ, Watanabe K, Ueda T, Indo HP, Suenaga S, Hisamitsu T, Ozawa T, Yasuhara H, Koide R.
  • Journal Title: Free Radic Res Volume: 47 Issue: 10 Pages: 774-780
  • DOI: 10.3109/10715762.2013.829570
  • Peer Reviewed
• [Journal Article] Comparison of in vivo and in vitro antioxidative parameters for eleven food factors (2013)
  • Author(s): H. P. Indo
  • Journal Title: RSC Advances Volume: 3 Issue: 14 Pages: 4535-4538
  • DOI: 10.1039/c3ra22686g
  • Peer Reviewed
• [Journal Article] Snail modulates cell metabolism in MDCK cells. (2013)
  • Author(s): Haraguchi M
  • Journal Title: Biochem Biophys Res Commun. Volume: 432 Issue: 4 Pages: 618-625
  • DOI: 10.1016/j.bbrc.2013.02.035
  • Peer Reviewed
• [Journal Article] Roles of mitochondria-generated reactive oxygen species on X-ray-induced apoptosis in a human hepatocellular carcinoma cell line, HLE. (2012)
  • Author(s): Indo HP, Inanami O, Koumura T, Suenaga S, Yen H-C, Kakinuma S, Matsumoto K, Nakanishi I, St Clair W, St Clair DK, Matsui H, Cornette R, Gusev O, Okuda T, Nakagawa Y, Ozawa T & Majima HJ
  • Journal Title: Free Radical Research Volume: Vol. 46, No. 8 Issue: 8 Pages: 1029-1043
  • DOI: 10.3109/10715762.2012.698012
  • Peer Reviewed
• [Journal Article] Rebamipide attenuates nonsteroidal anti-inflammatory drugs (NSAID) induced lipid peroxidation by the manganese superoxide dismutase (MnSOD) overexpression in gastrointestinal epithelial cells. (2012)
  • Author(s): Nagano Y, Matsui H, Shimokawa O, Hirayama A, Tamura M, Nakamura Kaneko T, Rai K, Indo HP, Majima HJ, Hyodo I
  • Journal Title: J Physiol Pharmacol. Volume: 63 Pages: 137-42
  • Peer Reviewed
• [Journal Article] Mitochondria as Possible Pharmaceutical Targets for the Effects of Vitamin E and its Homologues in Oxidative Stress-Related Diseases (2011)
  • Author(s): DOI: 10.1007/s00535-011-0434-6
  • Journal Title: Curr Pharm Des Volume: 17(21) Issue: 21 Pages: 2190-2195
  • DOI: 10.2174/138161211796957490
  • Peer Reviewed
• [Journal Article] Lansoprazole inhibits mitochondrial superoxide production and cellular lipid peroxidation induced by indomethacin in RGM1 cells (2011)
  • Author(s): Rai K, Matsui H, Kaneko T, Nagano Y, Shimokawa O, Udo J, Hirayama A, Hyodo I, Indo HP, Majima HJ
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 49 Issue: 1 Pages: 25-30
  • DOI: 10.3164/jcbn.10-133
  • NAID: 130004879258
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed
• [Journal Article] Gastric acid induces mitochondrial superoxide production and lipid peroxidation in gastric evithelial cells (2011)
  • Author(s): Matsui H.
  • Journal Title: J Gastroenterology Volume: 46 Issue: 10 Pages: 1167-1176
  • DOI: 10.1007/s00535-011-0434-6
  • Peer Reviewed
• [Presentation] Rho0 cells are susceptible to X-irradiation-induced cellular injury (2014)
  • Author(s): 37) Hiroko P. Indo, Chizuru Tsuruoka, Shizuko Kakinuma, Hirofumi Matsui, and Hideyuki J. Majima.
  • Organizer: SFRBM'S 21th Annual Meeting
  • Place of Presentation: Seattle, USA
  • Year and Date: 2014-11-19 – 2014-11-23
• [Presentation] fMRIによる顎関節症の疼痛症状に対する脳賦活部位の解析 (2014)
  • Author(s): 末永重明、永山邦宏、犬童寛子、宮脇正一、馬嶋秀行
  • Organizer: 第27回 日本顎関節学会 総会・学術大会
  • Place of Presentation: 福岡 九州大学医学部百年講堂
  • Year and Date: 2014-07-19 – 2014-07-20
• [Presentation] Quercetin Induced Starvation-like Condition Followed by Autophagy in Rat Gastric Tumor Cells. (2013)
  • Author(s): Hiroko P. Indo, Hirofumi Matsui, Hsiu-Chuan Yen, Toshihiko Ozawa, Aaron K Holly, Daret K St. Clair and Hideyuki J. Majima
  • Organizer: SFRBM'S 20th Annual Meeting
  • Place of Presentation: San antonio ,USA
• [Presentation] 顎関節症における咀嚼筋部疼痛に対する脳賦活部位のfMRI解析． (2013)
  • Author(s): 末永重明，永山邦宏，犬童寛子，宮脇正一，中村典史，馬嶋秀行
  • Organizer: 第６７回 NPO法人日本口腔科学会学術大会．栃木県総合文化センター
  • Place of Presentation: 宇都宮市、栃木
• [Presentation] X線および電子線による放射線治療が成功した口唇癌４症例の検討 (2013)
  • Author(s): 河野一典、川畑義裕、犬童寛子、末永重明、佐藤強志、杉原一正、平木嘉幸、馬嶋秀行
  • Organizer: 日本歯科放射線学会 第54回総会・学術大会
  • Place of Presentation: 福岡市、福岡
• [Presentation] MnSODによるＸ線由来アポトーシスの抑制はそのミトコンドリア局在を必要とする， (2012)
  • Author(s): 犬童寛子、馬嶋秀行
  • Organizer: 第57回（社）日本口腔外科学会総会・学術大会
  • Place of Presentation: 横浜
• [Presentation] Quercetin Induced Autophagy by Starvation Simulated Condition in Rat Gastric Tumor Cell Line, RGK-1. (2012)
  • Author(s): Hiroko P Indo, Sompong Sriburee, Samlee Mankhetkorn, Hsiu-Chuan Yen, Hirofumi Matsui, Toshihiko Ozawa , and Hideyuki J Majima
  • Organizer: The 16th Biennial Meeting of the Society for Free Radical Research
  • Place of Presentation: London,UK
• [Presentation] Role of free radical generated from mitochondria on sulfhydryl residues of oxidative stress signal-related protein (2012)
  • Author(s): Hiroko P. Indo, Tessa Barrett, Clare L.Hawkins, Michael Davies and Hideyuki J. Majima
  • Organizer: International Redox Core Symposium
  • Place of Presentation: Nishitetsu Grand Hotel, Fukuoka
• [Presentation] ケルセチンによってオートファジーが誘導される (2011)
  • Author(s): 犬童寛子、Sompong Sriburee、富田和男、松井裕史、小澤俊彦、馬嶋秀行
  • Organizer: 第64回日本酸化ストレス学会学術集会
  • Place of Presentation: ルスツリゾートホテル＆コンベンション 北海道
• [Presentation] 宇宙放射線および微小重力が哺乳細胞に及ぼす影響 (2011)
  • Author(s): 犬童寛子、 石岡憲昭、鈴木ひろみ、島津徹、矢野幸子、谷垣史章、桝田大輔、蓑部悦子、亀山正樹、馬嶋秀行
  • Organizer: 第64回日本酸化ストレス学会学術集会
  • Place of Presentation: ルスツリゾートホテル＆コンベンション 北海道
• [Presentation] Effects of Indometahcin on rat gastric normal cells RGM-1 and its tumourized cells RGK-1 and the prevention by Rebamipide (2011)
  • Author(s): Motoharu Miyamoto, Hiroko P. Indo, Kazuyuki Noguchi, Hciu-Chuan Yen, Hirofumi Matsui, Toshihiko Ozawa and Hideyuki J. Majima
  • Organizer: 5th SFRR-Asia,8th ASMRM,11th J-mit
  • Place of Presentation: Kagoshima Citizens' Culture Hall ,Kagoshiama
• [Presentation] Induction of mitochondrial ROS and cell death by cytokines and its prevention by estrogen in rheumatoid arthritis fibroblasts (2011)
  • Author(s): Shigeaki Suenaga, Hiroko P. Indo, Hsiu-Chuan Yen, Kosei Ijiri, Setsuro Komiya, Takami Matsuyama, Masahiro Higuchi, Hirofumi Matsui, Toshihiko Ozawa, Hideyuki J. Majima
  • Organizer: 5th SFRR-Asia,8th ASMRM,11th J-mit
  • Place of Presentation: Kagoshima Citizens' Culture Hall ,Kagoshima
• [Presentation] Effects of 14 and 28 days exposure of human neuro cells in Space; Neuro Rad Experiments (2011)
  • Author(s): Hiroko P. Indo, Noriaki Ishioka, Hiromi Suzuki, Toru Shimazu, Sachiko Yano, Fumiaki Tanigaki, Aiko Nagamatsu,Daisuke Masuda, Etsuko Minobe, Masaki Kameyama and Hideyuki J. Majima1)
  • Organizer: SFRBM's 18th Annual Meeting
  • Place of Presentation: Sheraton Atlanta Hotel, Atlanta GA
• [Presentation] ρ0 cells are susceptible to X-irradiation.
  • Author(s): Hiroko P Indo, Chizuru Tsuruoka, Shizuko Kakinuma, Masao Suzuki, Ken-ichiro Matsumoto, Ikuo Nakanishi, Hsiu-Chuan Yen, Hirofumi Matsui, Toshihiko Ozawa, Hideyuki J Majima
  • Organizer: The 32nd Annual Meeting of Japanese Society for Cytoprotection & Cytobiology.
  • Place of Presentation: kyoto, Japan