SNP typing of the cariogenic protein gene
Project/Area Number |
23592790
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Conservative dentistry
|
Research Institution | Hokkaido University |
Principal Investigator |
|
Co-Investigator(Renkei-kenkyūsha) |
OHTA Toru 北海道医療大学, 個体差健康科学研究所, 教授 (10223885)
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
|
Keywords | 遺伝子多型 / 遺伝子分析 / う蝕感受性 / 唾液緩衝能 / 齲蝕 / PCR高解像度融解曲線分析 |
Research Abstract |
To evaluate the discrimination method of HLA Alleles, we developed the detection method by PCR-Sequence Specific Primer (SSP) and the melt peak analysis (Tm). The frequencies of target Allele calculated from database were less than 10%, therefore our method could discriminate more than 90% samples. We analyzed the Buffer capacity of saliva and Carbonic Anhydrase VI SNP of Children attended permanent caries preventing program. There were a positive association between caries present and buffer capacity. We could distinguish the rs2274327 (C/T) polymorphism by PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) method.
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Report
(4 results)
Research Products
(8 results)