SNP typing of the cariogenic protein gene

• Project/Area Number: 23592790
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Hokkaido University
• Principal Investigator: MATSUDA Yasuhiro 北海道大学, 大学病院, 助教 (50431317)
• Co-Investigator(Renkei-kenkyūsha): OHTA Toru 北海道医療大学, 個体差健康科学研究所, 教授 (10223885)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 遺伝子多型 / 遺伝子分析 / う蝕感受性 / 唾液緩衝能 / 齲蝕 / PCR高解像度融解曲線分析

Research Abstract

To evaluate the discrimination method of HLA Alleles, we developed the detection method by PCR-Sequence Specific Primer (SSP) and the melt peak analysis (Tm). The frequencies of target Allele calculated from database were less than 10%, therefore our method could discriminate more than 90% samples.
We analyzed the Buffer capacity of saliva and Carbonic Anhydrase VI SNP of Children attended permanent caries preventing program. There were a positive association between caries present and buffer capacity. We could distinguish the rs2274327 (C/T) polymorphism by PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) method.

Research Products

• [Journal Article] Effective Screening for the HLA-B*35:05 and HLA-B*57:01 Alleles by Using an Allele-Specific Polymerase Chain Reaction Melting Assay (2014)
  • Author(s): Yasuhiro Matsuda, Hidehiko Sano, Yuichi Iwaki
  • Journal Title: 2013 International Conference on Biological, Medical and Chemical Engineering (BMCE 2013) Volume: Session 2 Pages: 160-168
  • Peer Reviewed
• [Journal Article] う蝕と遺伝子解析 (2013)
  • Author(s): 松田康裕
  • Journal Title: 北海道歯誌 Volume: 33 Pages: 50-52
  • NAID: 10031165909
  • Peer Reviewed
• [Journal Article] Effective Screening for the HLA-B*35: 05 and HLA-B*57: 01 Alleles by Using an Allele-Specific Polymerase Chain Reaction Melting Assay (2013)
  • Author(s): Yasuhiro Matsuda, Hidehiko Sano, Yuichi Iwaki
  • Journal Title: 2013 International Conference on Biological, Medical and Chemical Engineering (BMCE 2013) Volume: SESSION 2: BIOLOGICAL SCIENCE AND ENGINEERING APPLICATIONS Pages: 160-168
  • Peer Reviewed
• [Presentation] Comparison of Fluoride Regimens on Fluorine Uptake in Carious Enamel (2013)
  • Author(s): Naoki HASHIMOTO, Yoshiki FUNATO, Saiko OKI, Yasuhiro. MATSUDA, Katsushi OKUYAMA, Hiroko YAMAMOTO, Hisanori KOMATSU, and Hidehiko SANO
  • Organizer: IADR 2013 General Session
  • Place of Presentation: Washington Convention Center(Seattle, USA)
  • Year and Date: 2013-03-22
• [Presentation] Demineralize Prevention of Dentin With Fluoride Varnish via Automatic pH-cycling (2013)
  • Author(s): Saiko OKI, Yasuhiro MATSUDA, Naoki HASHIMOTO, Katsushi OKUYAMA, Hiroko YAMAMOTO, Yoshiki FUNATO, Hisanori KOMATSU, and Hidehiko SANO
  • Organizer: IADR 2013 General Session
  • Place of Presentation: Washington Convention Center(Seattle, USA)
  • Year and Date: 2013-03-22
• [Presentation] Effective Screening for the HLA-B*35: 05 and HLA-B*57: 01 Alleles by Using an Allele-Specific Polymerase Chain Reaction Melting Assay (2013)
  • Author(s): Yasuhiro Matsuda, Hidehiko Sano, Yuichi Iwaki, Iwaki
  • Organizer: Medical and Chemical Engineering (BMCE 2013)
  • Place of Presentation: Royal Park Hotel (Hong Kong, China)
• [Presentation] Demineralize Prevention of Dentin With Fluoride Varnish via Automatic pH-cycling (2013)
  • Author(s): S. OKI, Y. MATSUDA, N. HASHIMOTO, K. OKUYAMA, H. YAMAMOTO, Y. FUNATO, H. KOMATSU, and H. SANO
  • Organizer: International Association for Dental Research
  • Place of Presentation: Washington State Convention Center (U.S.A)
• [Presentation] Comparison of Fluoride Regimens on Fluorine Uptake in Carious Enamel (2013)
  • Author(s): N. HASHIMOTO, Y. FUNATO, S. OKI, Y. MATSUDA, K. OKUYAMA, H. YAMAMOTO, H. KOMATSU, and H. SANO
  • Organizer: International Association for Dental Research
  • Place of Presentation: Washington State Convention Center (U.S.A)