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Profiling of Microbiota in Infected Root Canals Utilizing Metagenomic and Bioplorer Analyses: Their Clinical Application to the Evaluation of the Outcomes of Endodontic Treatment

Research Project

Project/Area Number 23592791
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Conservative dentistry
Research InstitutionTohoku University

Principal Investigator

SATO Takuichi  東北大学, 歯学研究科(研究院), 講師 (10303132)

Co-Investigator(Kenkyū-buntansha) MAYANGI Gen  東北大学, 大学院歯学研究科, 助教 (10451600)
YAMAKI Keiko  東北大学, 大学院歯学研究科, 助教 (90182419)
KAWAMURA Yoshiaki  愛知学院大学, 薬学部, 教授 (80262757)
MATSUYAMA Junko  新潟大学, 医歯学系, 助教 (30293236)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywords歯学 / 口腔細菌 / バイオフィルム / メタゲノム / 根尖性歯周炎
Research Abstract

The bacterial examination has been performed during the course of the root canal treatment. In this study, metagenomic and Bioplorer (using fluorescence reagents and a membrane filter) analyses were applied to the profiling of microbiota in infected root canals, in order to evaluate the outcomes of the treatment. As a result, detailed components of the microbiota and rapid quantification of bacteria in infected root canals were found to be successfully performed utilizing these two methods.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (72 results)

All 2014 2013 2012 2011 Other

All Journal Article (15 results) (of which Peer Reviewed: 15 results,  Open Access: 1 results) Presentation (41 results) (of which Invited: 2 results) Book (16 results)

  • [Journal Article] Detection and identification of oral anaerobes from intraoperative bronchial fluids of patients with pulmonary carcinoma2014

    • Author(s)
      Hasegawa A, Sato T, Hoshikawa Y, Ishida N, Tanda N, Kawamura Y, Kondo T, Takahashi N
    • Journal Title

      Microbiol Immunol

      Volume: 58(in press) Issue: 7 Pages: 375-381

    • DOI

      10.1111/1348-0421.12157

    • NAID

      40020130159

    • Related Report
      2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Responses of infected dental pulp toαTCP containing antimicrobials in rat molars2014

    • Author(s)
      Sato T, Kenmotsu S, Nakakura-Ohshima K, Takahashi N, Ohshima H
    • Journal Title

      Arch Histol Cytol

      Volume: 75(in press)

    • URL

      https://www.jstage.jst.go.jp/browse/aohc

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Detection and identification of oral anaerobes from intraoperative bronchial fluids of patients with pulmonary carcinoma.2014

    • Author(s)
      Hasegawa A, Sato T, Hoshikawa Y, Ishida N, Tanda N, Kawamura Y, Kondo T, Takahashi N
    • Journal Title

      Microbiology and Immunology

      Volume: 58

    • NAID

      40020130159

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Responses of infected dental pulp to αTCP containing antimicrobials in rat molars.2014

    • Author(s)
      Sato T, Kenmotsu S, Nakakura-Ohshima K, Takahashi N, Ohshima H
    • Journal Title

      Archives of Histology and Cytology

      Volume: 75

    • NAID

      130005087018

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Responses of infected dental pulp to αTCP containing antimicrobials in rat molars.2013

    • Author(s)
      Sato T, Kenmotsu S, Nakakura-Ohshima K, Takahashi N, Ohshima H
    • Journal Title

      Arch Histol Cytol

      Volume: 75

    • NAID

      130005087018

    • Related Report
      2012 Research-status Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Quantification and identification of bacteria in acrylic resin denture bases and dento-maxillary obturator- prostheses2012

    • Author(s)
      Takeuchi Y, Nakajo K, Sato T, Koyama S, Sasaki K, Takahashi N
    • Journal Title

      Am J Dent

      Volume: 25(3)

    • URL

      http://www.amjdent.com/Archive/2012/June%202012%20Issue.html

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Microbial composition of dental plaque microflora on first molars with orthodontic bands2012

    • Author(s)
      Komori R, Sato T, Takano-Yamamoto T, Takahashi N
    • Journal Title

      J Oral Biosci

      Volume: 54 Issue: 2 Pages: 107-112

    • DOI

      10.1016/j.job.2012.01.009

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Rapid quantification of bacteria in infected root canals using fluorescence filter : A pilot study on its clinical application to the evaluation of the outcomes of endodontic treatment2012

    • Author(s)
      Sato T, Yamaki K, Ishida N, Shoji M, Sato E, Abiko Y, Hashimoto K, Takeuchi Y, Matsuyama J, Shimauchi H, Takahashi N
    • Journal Title

      Int J Dent

      Volume: Volume 2012(Article ID 172935) Pages: 4-4

    • DOI

      10.1155/2012/172935

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cultivable anaerobic microbiota of infected root canals2012

    • Author(s)
      Sato T, Yamaki K, Ishida N, Hashimoto K, Takeuchi Y, Shoji M, Sato E, Matsuyama J, Shimauchi H, Takahashi N
    • Journal Title

      Int J Dent

      Volume: Volume 2012(Article ID 609689) Pages: 5-5

    • DOI

      10.1155/2012/609689

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Microflora profiling of infected root canal before and after treatment using culture-independent methods2012

    • Author(s)
      Ito Y, Sato T, Yamaki K, Mayanagi G, Hashimoto K, Shimauchi H, Takahashi N
    • Journal Title

      J Microbiol

      Volume: 50(1) Issue: 1 Pages: 58-62

    • DOI

      10.1007/s12275-012-0459-4

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Quantification and identification of bacteria in acrylic resin denture bases and dento-maxillary obturator-prostheses.2012

    • Author(s)
      Takeuchi Y, Nakajo K, Sato T, Koyama S, Sasaki K, Takahashi N
    • Journal Title

      Am J Dent

      Volume: 25 Pages: 171-175

    • Related Report
      2012 Research-status Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Microbial composition of dental plaque microflora on first molars with orthodontic bands and brackets, and acidogenic potential of these bacteria.2012

    • Author(s)
      Komori R, Sato T, Takano-Yamamoto T, Takahashi N
    • Journal Title

      J Oral Biosci

      Volume: 55

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Profiling of dental plaque microflora on root caries lesions and the protein-denaturing activity of these bacteria2011

    • Author(s)
      Hashimoto K, Sato T, Shimauchi H, Takahashi N
    • Journal Title

      Am J Dent

      Volume: 24(5) Pages: 295-299

    • URL

      http://www.amjdent.com/Archive/2011/October%202011%20Issue.html

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Detection and identification of non-Candida albicans species in human oral lichen planus2011

    • Author(s)
      Masaki M, Sato T, Sugawara Y, Sasano T, Takahashi N
    • Journal Title

      Microbiol Immunol

      Volume: 55(1) Issue: 1 Pages: 66-70

    • DOI

      10.1111/j.1348-0421.2010.00285.x

    • NAID

      10031121442

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Profiling of dental plaque microflora on root caries lesions and the protein-denaturing activity of these bacteria.2011

    • Author(s)
      Hashimoto K, Sato T, Shimauchi H, Takahashi N
    • Journal Title

      Am J Dent

      Volume: 24 Pages: 295-299

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] A rapid and simple detection of plaque bacteria using a novel molecular method, PCR-PAS, and its clinical application2014

    • Author(s)
      Tian L, Sato T, Niwa K, Kawase M, Tanner ACR, Takahashi N
    • Organizer
      The 5th International Symposium for Interface Oral Health Science
    • Place of Presentation
      Sendai
    • Related Report
      2013 Final Research Report
  • [Presentation] Microbiota profiling of bronchial fluids of elderly patients. Innovative Research for Biosis-Abiosis Intelligent Interface Symposium2014

    • Author(s)
      Ishida N, Sato T, Hoshikawa Y, Tanda N, Kondo T, Sasaki K, Takahashi N
    • Organizer
      The 5th International Symposium for Interface Oral Health Science
    • Place of Presentation
      Sendai
    • Related Report
      2013 Final Research Report
  • [Presentation] Comprehensive analysis of indigenous plaque microbiota of pre- and post-weanling, and grown-up mice2014

    • Author(s)
      Matsuyama J, Sato T, Quispe-Salcedo A, Mayanagi G, Takahashi N, Ohshima H
    • Organizer
      The 5th International Symposium for Interface Oral Health Science
    • Place of Presentation
      Sendai
    • Related Report
      2013 Final Research Report
  • [Presentation] Porphyromonas new species candidate isolated from intraoperative bronchial fluids of patients with pulmonary carcinoma. Innovative Research for Biosis-Abiosis Intelligent Interface Symposium2014

    • Author(s)
      Kawamura Y, Sato T, Tomida J, Morita Y, Naka T, Fujiwara N, Tanaka K, Yoshida Y, Yoshimura F, Takahashi N
    • Organizer
      The 5th International Symposium for Interface Oral Health Science
    • Place of Presentation
      Sendai
    • Related Report
      2013 Final Research Report
  • [Presentation] Relationship between the consistency of food substances and the incidence of aspiration pneumonia of the elderly. Innovative Research for Biosis-Abiosis Intelligent Interface Symposium2014

    • Author(s)
      Sakashita R, Nishitani M, Ono H, Sato T, Hamada M
    • Organizer
      The 5th International Symposium for Interface Oral Health Science
    • Place of Presentation
      Sendai
    • Related Report
      2013 Final Research Report
  • [Presentation] Oral malodorous gasses of a perioperative patient with postoperative pneumonia. Innovative Research for Biosis-Abiosis Intelligent Interface Symposium2014

    • Author(s)
      Tanda N, Hoshikawa Y, Ishida N, Sato T, Takahashi N, Hosokawa R, Koseki T
    • Organizer
      The 5th International Symposium for Interface Oral Health Science
    • Place of Presentation
      Sendai
    • Related Report
      2013 Final Research Report
  • [Presentation] Oral microbiota in crevices around dental implants : profiling of the oral biofilm. Innovative Research for Biosis- Abiosis Intelligent Interface Symposium2014

    • Author(s)
      Sato T, Kawamura Y, Mayanagi G, Washio J, Takahashi N
    • Organizer
      The 5th International Symposium for Interface Oral Health Science
    • Place of Presentation
      Sendai
    • Related Report
      2013 Final Research Report
  • [Presentation] A rapid and simple detection of plaque bacteria using a novel molecular method, PCR-PAS, and its clinical application.2014

    • Author(s)
      Tian L, Sato T, Niwa K, Kawase M, Tanner ACR, Takahashi N
    • Organizer
      Innovative Research for Biosis-Abiosis Intelligent Interface Symposium
    • Place of Presentation
      仙台
    • Related Report
      2013 Annual Research Report
  • [Presentation] Microbiota profiling of bronchial fluids of elderly patients.2014

    • Author(s)
      Ishida N, Sato T, Hoshikawa Y, Tanda N, Kondo T, Sasaki K, Takahashi N
    • Organizer
      Innovative Research for Biosis-Abiosis Intelligent Interface Symposium
    • Place of Presentation
      仙台
    • Related Report
      2013 Annual Research Report
  • [Presentation] Comprehensive analysis of indigenous plaque microbiota of pre- and post-weanling, and grown-up mice.2014

    • Author(s)
      Matsuyama J, Sato T, Quispe-Salcedo A, Mayanagi G, Takahashi N, Ohshima H
    • Organizer
      Innovative Research for Biosis-Abiosis Intelligent Interface Symposium
    • Place of Presentation
      仙台
    • Related Report
      2013 Annual Research Report
  • [Presentation] Porphyromonas new species candidate isolated from intraoperative bronchial fluids of patients with pulmonary carcinoma.2014

    • Author(s)
      Kawamura Y, Sato T, Tomida J, Morita Y, Naka T, Fujiwara N, Tanaka K, Yoshida Y, Yoshimura F, Takahashi N
    • Organizer
      Innovative Research for Biosis-Abiosis Intelligent Interface Symposium
    • Place of Presentation
      仙台
    • Related Report
      2013 Annual Research Report
  • [Presentation] Relationship between the consistency of food substances and the incidence of aspiration pneumonia of the elderly.2014

    • Author(s)
      Sakashita R, Nishitani M, Ono H, Sato T, Hamada M
    • Organizer
      Innovative Research for Biosis-Abiosis Intelligent Interface Symposium
    • Place of Presentation
      仙台
    • Related Report
      2013 Annual Research Report
  • [Presentation] Oral malodorous gasses of a perioperative patient with postoperative pneumonia.2014

    • Author(s)
      Tanda N, Hoshikawa Y, Ishida N, Sato T, Takahashi N, Hosokawa R, Koseki T
    • Organizer
      Innovative Research for Biosis-Abiosis Intelligent Interface Symposium
    • Place of Presentation
      仙台
    • Related Report
      2013 Annual Research Report
  • [Presentation] 口腔フローラのプロファイリング2013

    • Author(s)
      佐藤拓一,河村好章
    • Organizer
      第50回日本細菌学会中部支部総会
    • Place of Presentation
      愛知県蒲郡市
    • Year and Date
      2013-10-18
    • Related Report
      2013 Final Research Report
  • [Presentation] 離乳前後および成熟マウスの口腔内プラーク常在菌叢の網羅的解析2013

    • Author(s)
      松山順子,佐藤拓一,Angela Quispe- Salcedo,高橋信博,大島勇人
    • Organizer
      第55回歯科基礎医学会学術大会
    • Place of Presentation
      岡山
    • Year and Date
      2013-09-22
    • Related Report
      2013 Final Research Report
  • [Presentation] The Front Line of Research on Oral Microbiota : The challenge reports by young investigators2013

    • Author(s)
      大島朋子、浜田信城、宮川博史、佐藤拓一(企画・座長)
    • Organizer
      第55回歯科基礎医学会学術大会サテライトシンポジウム(SS-8)
    • Place of Presentation
      岡山
    • Year and Date
      2013-09-20
    • Related Report
      2013 Final Research Report
  • [Presentation] Microbiota profiling of bronchial fluids of elderly patients2013

    • Author(s)
      Ishida N, Sato T, Hoshikawa Y, Tanda N, Kondo T, Takahashi N
    • Organizer
      第55回歯科基礎医学会学術大会サテライトシンポジウム
    • Place of Presentation
      岡山
    • Year and Date
      2013-09-20
    • Related Report
      2013 Final Research Report
  • [Presentation] Hydrogen sulfide, methyl mercaptan, and acetaldehyde in oral health care for perioperative patients with pulmonary carcinoma2013

    • Author(s)
      Tanda N, Ishida N, Hoshikawa Y, Sato T, Takahashi N, Hosokawa R, Koseki T
    • Organizer
      第55回歯科基礎医学会学術大会サテライトシンポジウム
    • Place of Presentation
      岡山
    • Year and Date
      2013-09-20
    • Related Report
      2013 Final Research Report
  • [Presentation] Molecular biological profiling of oral biofilm : Quantitative and qualitative analyses2013

    • Author(s)
      Sato T, Kawamura Y, Ishida N, Yamaki K, Matsuyama J, Takahashi N
    • Organizer
      The 2nd IADR-APR (Asia Pacific Region): JADR Mini-Symposium
    • Place of Presentation
      Bangkok, Thailand
    • Year and Date
      2013-08-21
    • Related Report
      2013 Final Research Report
  • [Presentation] Profiling of oral biofilm microbiota utilizing molecular biological methods2013

    • Author(s)
      Sato T, Kawamura Y, Yamaki K, Shimauchi H, Takahashi N
    • Organizer
      Innovative Research for Biosis-Abiosis Intelligent Interface
    • Place of Presentation
      China
    • Year and Date
      2013-07-26
    • Related Report
      2013 Final Research Report
  • [Presentation] Analysis of hydrogen sulfide, methyl mercaptan, and acetaldehyde in oral health care for perioperative patients of lung cancer2013

    • Author(s)
      Tanda N, Ishida N, Hoshikawa Y, Sato T, Takahashi N, Hosokawa R, Koseki T
    • Organizer
      MASCC (The Multinational Association of Supportive Care in Cancer)
    • Place of Presentation
      Berlin, Germany
    • Related Report
      2013 Final Research Report
  • [Presentation] Analysis of hydrogen sulfide, methyl mercaptan, and acetaldehyde in oral health care for perioperative patients of lung cancer.2013

    • Author(s)
      Tanda N, Ishida N, Hoshikawa Y, Sato T, Takahashi N, Hosokawa R, Koseki T
    • Organizer
      MASCC (The Multinational Association of Supportive Care in Cancer)
    • Place of Presentation
      Berlin, Germany
    • Related Report
      2013 Annual Research Report 2012 Research-status Report
  • [Presentation] 蛍光フィルターを利用した、感染根管内細菌の迅速定量検出法2012

    • Author(s)
      庄司恵,佐藤愛美加,佐藤拓一,石田直子,八巻惠子,高橋信博
    • Organizer
      第22回日本歯科医学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2012-11-11
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] 口腔バイオフィルムの分子生物学的プロファイリング:メタゲノム解析2012

    • Author(s)
      佐藤拓一,河村好章,八巻惠子,島内英俊,高橋信博
    • Organizer
      第22回日本歯科医学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2012-11-10
    • Related Report
      2013 Final Research Report
  • [Presentation] マウス口腔内プラーク常在菌叢の網羅的解析2012

    • Author(s)
      松山順子,佐藤拓一,Angela Quispe- Salcedo,石田直子,高橋信博,大島勇人
    • Organizer
      第54回歯科基礎医学会学術大会
    • Place of Presentation
      郡山
    • Year and Date
      2012-09-16
    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Presentation] The oral microbiome and biofilm research : new concepts and new approaches by presentation by young researchers2012

    • Author(s)
      佐藤拓一,大島朋子,宮川博史,浜田信城(企画・座長)
    • Organizer
      第54回歯科基礎医学会学術大会サテライトシンポジウム
    • Place of Presentation
      郡山
    • Year and Date
      2012-09-14
    • Related Report
      2013 Final Research Report
  • [Presentation] 口腔マイクロバイオームおよびバイオフィルム研究:研究の最先端と若手のチャレンジ2012

    • Author(s)
      佐藤拓一,大島朋子,宮川博史,浜田信城:(オーガナイザー)
    • Organizer
      第54回歯科基礎医学会学術大会サテライトシンポジウム
    • Place of Presentation
      郡山
    • Related Report
      2012 Research-status Report
  • [Presentation] 口腔バイオフィルムの分子生物学的プロファイリング-メタゲノム解析-2012

    • Author(s)
      佐藤拓一,河村好章,八巻惠子,島内英俊,高橋信博
    • Organizer
      第22回日本歯科医学会総会
    • Place of Presentation
      大阪
    • Related Report
      2012 Research-status Report
  • [Presentation] 高齢者の歯肉縁下プラーク細菌叢:Porphyromonas gingivalisの定量解析とfimA遺伝子型タイピング2011

    • Author(s)
      安彦友希,佐藤拓一,坂下玲子,高橋信博
    • Organizer
      第53回歯科基礎医学会学術大会
    • Place of Presentation
      岐阜
    • Year and Date
      2011-10-02
    • Related Report
      2013 Final Research Report
  • [Presentation] Intensive perioperative oral care prevents postoperative pneumonia after lung resection2011

    • Author(s)
      Hoshikawa Y, Tanda N, Endo H, Sato T, Hasegawa A, Kondo T
    • Organizer
      American Thoracic Society 2011 International Conference
    • Place of Presentation
      Denver, Colorado, USA
    • Year and Date
      2011-05-17
    • Related Report
      2013 Final Research Report
  • [Presentation] 感染根管内細菌叢のpyrosequencing法によるメタゲノム解析2011

    • Author(s)
      佐藤拓一,河村好章,八巻惠子,島内英俊,高橋信博
    • Organizer
      第53回歯科基礎医学会学術大会
    • Place of Presentation
      岐阜
    • Related Report
      2011 Research-status Report
  • [Presentation] 高齢者の歯肉縁下プラーク細菌叢:Porphyromonas gingivalis の定量解析とfimA 遺伝子型タイピング2011

    • Author(s)
      安彦友希,佐藤拓一,坂下玲子,高橋信博
    • Organizer
      第53回歯科基礎医学会学術大会
    • Place of Presentation
      岐阜
    • Related Report
      2011 Research-status Report
  • [Presentation] Profiling of oral biofilm microbiota utilizing molecular biological methods.

    • Author(s)
      Sato T, Kawamura Y, Yamaki K, Shimauchi H, Takahashi N
    • Organizer
      Peking-Tohoku Dental Symposium: Innovative Research for Biosis-Abiosis Intelligent Interface
    • Place of Presentation
      Beijing, China
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Molecular biological profiling of oral biofilm: Quantitative and qualitative analyses.

    • Author(s)
      Sato T, Kawamura Y, Ishida N, Yamaki K, Matsuyama J, Takahashi N
    • Organizer
      The 2nd IADR-APR (Asia Pacific Region)
    • Place of Presentation
      Bangkok, Thailand
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Microbiota profiling of bronchial fluids of elderly patients.

    • Author(s)
      Ishida N, Sato T, Hoshikawa Y, Tanda N, Kondo T, Takahashi N
    • Organizer
      第55回歯科基礎医学会学術大会サテライトシンポジウム
    • Place of Presentation
      岡山
    • Related Report
      2013 Annual Research Report
  • [Presentation] Hydrogen sulfide, methyl mercaptan, and acetaldehyde in oral health care for perioperative patients with pulmonary carcinoma.

    • Author(s)
      Tanda N, Ishida N, Hoshikawa Y, Sato T, Takahashi N, Hosokawa R, Koseki T
    • Organizer
      第55回歯科基礎医学会学術大会サテライトシンポジウム
    • Place of Presentation
      岡山
    • Related Report
      2013 Annual Research Report
  • [Presentation] Oral microbiota in crevices around dental implants: profiling of the oral biofilm.

    • Author(s)
      Sato T, Kawamura Y, Mayanagi G, Washio J, Takahashi N
    • Organizer
      Innovative Research for Biosis-Abiosis Intelligent Interface Symposium
    • Place of Presentation
      仙台
    • Related Report
      2013 Annual Research Report
  • [Presentation] Comprehensive analysis of indigenous plaque microbiota in mice.

    • Author(s)
      Matsuyama J, Sato T, Ishimaru H, Muraoka K, Yamashita Y, Quispe-Salcedo A, Mayanagi G, Takahashi N, Ohshima H
    • Organizer
      The 2nd IADR-APR (Asia Pacific Region)
    • Place of Presentation
      Bangkok, Thailand
    • Related Report
      2013 Annual Research Report
  • [Presentation] Microbiota profiling of intraoperative bronchial fluids in elderly patients with pulmonary carcinoma.

    • Author(s)
      Ishida N, Sato T, Hoshikawa Y, Tanda N, Kondo T, Sasaki K, Takahashi N
    • Organizer
      The 2nd IADR-APR (Asia Pacific Region)
    • Place of Presentation
      Bangkok, Thailand
    • Related Report
      2013 Annual Research Report
  • [Presentation] 離乳前後および成熟マウスの口腔内プラーク常在菌叢の網羅的解析

    • Author(s)
      松山順子,佐藤拓一,Angela Quispe-Salcedo,高橋信博,大島勇人
    • Organizer
      第55回歯科基礎医学会学術大会
    • Place of Presentation
      岡山
    • Related Report
      2013 Annual Research Report
  • [Presentation] 口腔フローラのプロファイリング

    • Author(s)
      佐藤拓一,橋本紀洋,河村好章
    • Organizer
      第50回日本細菌学会中部支部総会
    • Place of Presentation
      愛知県蒲郡市
    • Related Report
      2013 Annual Research Report
  • [Book] 第10章口腔内微生物叢と歯面のバイオフィルム(The oral microflora and biofilms on teeth)(In :「デンタルカリエス―その病態と臨床マネージメント原著第2版(Dental Caries -The Disease and its Clinical Management」)2013

    • Author(s)
      佐藤拓一(翻訳)
    • Publisher
      医歯薬出版,東京
    • Related Report
      2013 Final Research Report
  • [Book] 第31章齲蝕に関する臨床的方針のばらつき(Variation in clinical decision making related to caries)(In :「デンタルカリエス―その病態と臨床マネージメント原著第2版(Dental Caries -The Disease and its Clinical Management」)2013

    • Author(s)
      佐藤拓一(翻訳)
    • Publisher
      医歯薬出版,東京
    • Related Report
      2013 Final Research Report
  • [Book] 口腔バイオフィルムの分子生物学的プロファイリング:メタゲノム解析(歯界展望特別号)2013

    • Author(s)
      佐藤拓一,河村好章,八巻惠子,島内英俊,高橋信博
    • Total Pages
      190
    • Publisher
      医歯薬出版
    • Related Report
      2013 Final Research Report
  • [Book] 蛍光フィルターを利用した、感染根管内細菌の迅速定量検出法(歯界展望特別号)2013

    • Author(s)
      庄司恵,佐藤愛美加,佐藤拓一,石田直子,八巻惠子,高橋信博
    • Total Pages
      294
    • Publisher
      医歯薬出版
    • Related Report
      2013 Final Research Report
  • [Book] 口腔内微生物叢と歯面のバイオフィルム In: 「デンタルカリエス―その病態と臨床マネージメント 原著第2版」2013

    • Author(s)
      佐藤拓一
    • Total Pages
      25
    • Publisher
      医歯薬出版
    • Related Report
      2013 Annual Research Report
  • [Book] 齲蝕に関する臨床的方針のばらつき In: 「デンタルカリエス―その病態と臨床マネージメント 原著第2版」2013

    • Author(s)
      佐藤拓一
    • Total Pages
      20
    • Publisher
      医歯薬出版
    • Related Report
      2013 Annual Research Report
  • [Book] 口腔バイオフィルムの分子生物学的プロファイリング:メタゲノム解析 In「歯界展望特別号」2013

    • Author(s)
      佐藤拓一,河村好章,八巻惠子,島内英俊,高橋信博
    • Total Pages
      364
    • Publisher
      医歯薬出版
    • Related Report
      2013 Annual Research Report
  • [Book] 蛍光フィルターを利用した、感染根管内細菌の迅速定量検出法 In「歯界展望特別号」2013

    • Author(s)
      庄司 恵,佐藤愛美加,佐藤拓一,石田直子,八巻惠子,高橋信博
    • Total Pages
      364
    • Publisher
      医歯薬出版
    • Related Report
      2013 Annual Research Report
  • [Book] 口腔内微生物叢と歯面のバイオフィルム In: デンタルカリエス―その病態とクリニカルマネージメント 第2版2013

    • Author(s)
      佐藤拓一(翻訳)
    • Publisher
      医歯薬出版
    • Related Report
      2012 Research-status Report
  • [Book] Subgingival plaque biofilm microflora of elderly subjects : quantitative analysis of Porphyromonas gingivalis and genotyping of its virulence-associated fimA(In : K. Sasaki, O. Suzuki, N. Takahashi (eds.) Interface Oral Health Science 2011)2012

    • Author(s)
      Abiko Y, Sato T, Sakashita R, Takahashi N
    • Publisher
      Springer, New York
    • Related Report
      2013 Final Research Report
  • [Book] Silent aspiration of oral bacteria : Microbiological analysis of intraoperative bronchial fluids from patients with pulmonary carcinoma2012

    • Author(s)
      Hasegawa A, Sato T, Hoshikawa Y, Kondo T, Takahashi N
    • Publisher
      Springer, New York
    • Related Report
      2013 Final Research Report
  • [Book] A method for quantitatively evaluating plaque biofilm removing capacity of a dental water jet using EPMA2012

    • Author(s)
      Kato K, Tamura K, Nakagaki H, Sakakibara S, Ou Y, Matsumoto S, Fujita K, Sato T
    • Publisher
      Springer, New York
    • Related Report
      2013 Final Research Report
  • [Book] Quantification and identification of bacteria in the maxillary obturator-prostheses(In : K. Sasaki, O. Suzuki, N. Takahashi (eds.) Interface Oral Health Science 2011)2012

    • Author(s)
      Takeuchi Y, Nakajo K, Sato T, Sakuma Y, Koyama S, Sasaki K, Takahashi N
    • Publisher
      Springer, New York
    • Related Report
      2013 Final Research Report
  • [Book] Change in infected root canal microflora during the course of root canal therapy( In : K. Sasaki, O. Suzuki, N. Takahashi (eds.) Interface Oral Health Science 2011)2012

    • Author(s)
      Yamaki K, Sato T, Hasegawa A, Abiko Y, Hashimoto K, Takeuchi Y, Matsuyama J, Shimauchi H, Takahashi N
    • Publisher
      Springer, New York
    • Related Report
      2013 Final Research Report
  • [Book] Community oral health promotion program fostering self-management for elderly people(In : K. Sasaki, O. Suzuki, N. Takahashi (eds.) Interface Oral Health Science 2011)2012

    • Author(s)
      Nishihira T, Nishitani M, Sato T, Abiko Y, Matsushita K, Hamada M, Sakashita R
    • Publisher
      Springer, New York
    • Related Report
      2013 Final Research Report
  • [Book] Interface Oral Health Science 20112012

    • Author(s)
      Yamaki K, Sato T, Hasegawa A, Abiko Y, Hashimoto K, Takeuchi Y, Matsuyama J, Shimauchi H, Takahashi N
    • Publisher
      Springer
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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