Profiling of Microbiota in Infected Root Canals Utilizing Metagenomic and Bioplorer Analyses: Their Clinical Application to the Evaluation of the Outcomes of Endodontic Treatment
| Project/Area Number |
23592791
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
SATO Takuichi 東北大学, 歯学研究科(研究院), 講師 (10303132)
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| Co-Investigator(Kenkyū-buntansha) |
MAYANGI Gen 東北大学, 大学院歯学研究科, 助教 (10451600)
YAMAKI Keiko 東北大学, 大学院歯学研究科, 助教 (90182419)
KAWAMURA Yoshiaki 愛知学院大学, 薬学部, 教授 (80262757)
MATSUYAMA Junko 新潟大学, 医歯学系, 助教 (30293236)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | 歯学 / 口腔細菌 / バイオフィルム / メタゲノム / 根尖性歯周炎 |
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| Research Abstract |
The bacterial examination has been performed during the course of the root canal treatment. In this study, metagenomic and Bioplorer (using fluorescence reagents and a membrane filter) analyses were applied to the profiling of microbiota in infected root canals, in order to evaluate the outcomes of the treatment. As a result, detailed components of the microbiota and rapid quantification of bacteria in infected root canals were found to be successfully performed utilizing these two methods.
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Report
(4 results)
Research Products
(72 results)
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[Journal Article] Rapid quantification of bacteria in infected root canals using fluorescence filter : A pilot study on its clinical application to the evaluation of the outcomes of endodontic treatment2012
Author(s)
Sato T, Yamaki K, Ishida N, Shoji M, Sato E, Abiko Y, Hashimoto K, Takeuchi Y, Matsuyama J, Shimauchi H, Takahashi N
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Journal Title
Int J Dent
Volume: Volume 2012(Article ID 172935)
Pages: 4-4
DOI
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Peer Reviewed
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[Book] Change in infected root canal microflora during the course of root canal therapy( In : K. Sasaki, O. Suzuki, N. Takahashi (eds.) Interface Oral Health Science 2011)2012
Author(s)
Yamaki K, Sato T, Hasegawa A, Abiko Y, Hashimoto K, Takeuchi Y, Matsuyama J, Shimauchi H, Takahashi N
Publisher
Springer, New York
Related Report
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