Usefulness on detection of intracanal microbe with photodynamic agent and laser

• Project/Area Number: 23592805
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Ohu University
• Principal Investigator: KIMURA Yuichi 奥羽大学, 歯学部, 教授 (60211877)
• Co-Investigator(Kenkyū-buntansha): IMAI Hiroaki 奥羽大学, 歯学部, 講師 (10265209)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 光増感剤 / 根管治療 / ナノ粒子 / 細菌検査 / レーザー / 臨床診断 / 蛍光色素 / 歯内療法

Research Abstract

Methylene blue as a photosensitizer, and poly (lactic-co-glycolic acid) (PLGA) spherical nanoparticles loaded with chitosan (particle size 250 nm) were used to combine microbe in this study. DIAGNOdent laser device (wavelength 655 nm) was used to detect photosensitizer with nanoparticles combined to microbe, and this study was performed within the nanoparticles concentration range of 0.01, 0.1, 1, 10 mg/ml. Detection sensitivity was slight increased using methylene blue-coated PLGA nanoparticles, but some problems still remain to complete diagnosis.

Research Products

• [Journal Article] Effects of blood contamination on sealing ability and micro- hardness of mineral trioxide aggregate used as a root-end filling material (2013)
  • Author(s): Sakaki S, Kimura Y, Imai H, Sato Y, Kamada A, Kurumada F, Yamazaki N, Yamada M, Amano Y, Masuda Y, Yamada Y, Koba K.
  • Journal Title: 日歯保存誌 Volume: 56 Pages: 200-207
  • NAID: 110009674429
  • Peer Reviewed
• [Journal Article] Effects of blood contamination on sealing ability and micro hardness of mineral trioxide affregate used as a root-end filling material (2013)
  • Author(s): Sasaki S, Kimura Y, Imai H, SatoY, Kamada A, Kurumada F, Yamazaki N, YamadaM, Amano Y, Masuda Y, Yamada Y, KobaK
  • Journal Title: 日本歯科保存学会雑誌 Volume: 56 Pages: 200-207
  • Peer Reviewed
• [Journal Article] Histological examination of experimentally infected root canals after preparation by Er : YAG laser irradiation (2011)
  • Author(s): Kimura Y, Tanabe M, Imai H, Amano Y, Masuda Y, Yamada Y.
  • Journal Title: Lasers Med Sci Volume: 26 Pages: 749-754
  • Peer Reviewed
• [Journal Article] Histological examination of experimentally infected root canals after preparation by Er:YAG laser irradiation (2011)
  • Author(s): Kimura Y, Tanabe M, Imai H, Amano Y, Masuda Y, Yamada Y
  • Journal Title: Lasers in Medical Science Volume: 26 Issue: 6 Pages: 749-754
  • DOI: 10.1007/s10103-010-0804-y
  • Peer Reviewed
• [Presentation] Effects of contamination on sealing ability and microhardness of mineral trioxide aggregate as a root-end filling material (2013)
  • Author(s): Kimura Y, Yamada Y, Masuda Y, and Koba K.
  • Organizer: The 9^<th> World Endodontic Congress
  • Place of Presentation: 東京
  • Year and Date: 2013-05-26
• [Presentation] Effects of contamination on sealing ability and micro hardness of mineral trioxide aggregate as a root-end filling material (2013)
  • Author(s): Kimura Y, YamadaY, Masuda Y, Koba K
  • Organizer: The 9th World Endodontic Congress
  • Place of Presentation: 東京