The study of the gene distribution and the role in hard tissue formation of amelogenin

Research Project

Project/Area Number	23592807
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Conservative dentistry
Research Institution	Showa University
Principal Investigator	YAMADA Yoshishige 昭和大学, 歯学部, 講師 (40360127)
Co-Investigator(Kenkyū-buntansha)	MASUDA Yoshiko 昭和大学, 歯学部, 准教 (10297038) KAWANAKA Takao 昭和大学, 歯学部, 助教 (10365702)
Project Period (FY)	2011 – 2013
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000) Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000) Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords	アメロジェニン / マウス胎仔 / エクソン / In situ hybridization法 / in situ hybridization法 / パラフィン切片 / in situ PCR / in situ LAMP / in situ hybridizatiom / 胎生マウス / マウス胎児 / PCR法 / in situ PCR法 / LAMP法
Research Abstract	According to the results of in situ hybridization, amelogenin gene expression was observed several non tooth related tissue such as brain, kidney, eye and bone related tissues. The amelogenin gene expression in these tissues, were a significant small amount of gene expression compared with gene expression in ameloblast of incisor. The tendency of the expression level of the amelogenin gene in the tissue they become strong in the late gestation than early fetal period has been confirmed.