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Regeneration of pulp-dentine complex using stem cells derived from oral mucosa or peripheral blood cell in alginic acid hybrid scaffold

Research Project

Project/Area Number 23592820
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Conservative dentistry
Research InstitutionOsaka Dental University

Principal Investigator

YOSHIKAWA Masataka  大阪歯科大学, 歯学部, 講師 (70148451)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords口腔粘膜由来細胞 / nodule形成 / アルギン酸 / 担体 / 骨形成 / オステオカルシン / ハイドロキシアパタイト / 口腔粘膜細胞 / 骨髄細胞 / 歯髄・象牙質複合体 / 歯の再生 / 幹細胞 / In vitro / Nodule形成 / 歯髄-象牙質複合体
Research Abstract

Oral mucosa-derived cells showed low rate of proliferation in the culture containing growth factors in the medium. However, nodule was formed.
In vivo study, bone marrow cells were suspended in alginic acid gel. The gel was filled in pores of HA scaffolds. The scaffolds were implanted in the subcutis of syngeneic rats. However, osteogenesis in the gel was not promoted. Therefore rat bone marrow cells were seeded in alginic acid sponge obtained by heat crosslinking. The sponge with the cells was inserted in the hollow center of cylindrical porous HA scaffold and implanted in rat subcutis for assessment of osteogenesis. As the result, quantity of osteocalcin significantly increased. Moreover, osteogenesis significantly increased quantitatively.
In hybrid scaffold composed of HA and formalin treated polyvinyl alcohol sponge coated with laminin, leucine, tryptophan or lysin, the in vivo examination revealed the significant quantitative increase of bone formation by bone marrow cells.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (42 results)

All 2014 2013 2012 2011 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 1 results) Presentation (37 results)

  • [Journal Article] Hybrid scaffolds composed of amino-acid coated sponge and hydroxyapatite for hard tissue formation by bone marrow cells2014

    • Author(s)
      Yabuuchi T、Yoshikawa M、Kakigi H、Hayashi H.
    • Journal Title

      Journal of Biomedical Science and Engineering

      Volume: Vol.7、No.6 Issue: 06 Pages: 316-329

    • DOI

      10.4236/jbise.2014.76034

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of laminin on hard tissue formation by bone marrow cells in vivo and in vitro2014

    • Author(s)
      Yoshikawa M、Kakigi H、Yabuuchi T、Hayashi H.
    • Journal Title

      Journal of Biomedical Science and Engineering

      Volume: Vol.7、No.1 Issue: 01 Pages: 15-23

    • DOI

      10.4236/jbise.2014.71003

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of L-lysine in culture medium on nodule formation by bone marrow cells2012

    • Author(s)
      Yoshikawa M、Shimomura Y、Kakigi H、Tsuji N、Yabuuchi T、Hayashi H.
    • Journal Title

      Journal of Biomedical Science and Engineering

      Volume: Vol.5、No.10 Issue: 10 Pages: 587-592

    • DOI

      10.4236/jbise.2012.510072

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Osteogenesis by bone marrow cells in a novel hybrid alginate/calcium phosphate sponge scaffold2012

    • Author(s)
      Kakigi H、Yoshikawa M、Hayashi H.
    • Journal Title

      Journal of Oral Tissue Engineering

      Volume: Vol. 9、No. 3 Pages: 113-125

    • NAID

      130004941176

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Osteogenesis by Bone Marrow Cells in a Novel Hybrid Alginate/2012

    • Author(s)
      Hideyuki Kakigi, Masataka Yoshikawa, Hiroyuki Hayashi
    • Journal Title

      Journal of Oral Tissue Engineering

      Volume: 9 Pages: 113-125

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Hybrid scaffolds composed of leucine-coated sponge and porous hydroxyapatite for hard tissue formation by bone marrow cells2014

    • Author(s)
      Masataka Yoshikawa, Hideyuki Kakigi Takanori Yabuuchi, Hiroyuki Hayashi
    • Organizer
      The 5th International Conference on Tissue Engineering
    • Place of Presentation
      Kos, Greece
    • Related Report
      2013 Annual Research Report
  • [Presentation] 骨髄幹細胞による硬組織形成に及ぼすアミノ酸の効果2013

    • Author(s)
      柿木栄幸、好川正孝、辻則正、林宏行
    • Organizer
      日本歯科保存学会2013年度秋季学術大会(139回)
    • Place of Presentation
      秋田市
    • Year and Date
      2013-10-18
    • Related Report
      2013 Final Research Report
  • [Presentation] Hard tissue formation by bone marrow cells in hybrid scaffold with tryptophan or lysine coating sponge / hydroxyapatite2013

    • Author(s)
      Yoshikawa M、Kakigi H、Yabuuchi T、Tsuji N、Hayashi H.
    • Organizer
      International Bone -Tissue -Engneering Congress 2013
    • Place of Presentation
      Singapore
    • Related Report
      2013 Final Research Report
  • [Presentation] Effects of two amino acids for hard tissue formation by bone marrow stem cells in vitro2013

    • Author(s)
      Kakigi H、Yoshikawa M、Tsuji N、Hayashi H.
    • Organizer
      International Bone -Tissue -Engneering Congress 2013
    • Place of Presentation
      Singapore
    • Related Report
      2013 Final Research Report
  • [Presentation] Hard tissue formation by bone marrow cells in tryptophan-coated sponge scaffolds2013

    • Author(s)
      Yoshikawa M、Kakigi H、Tsuji N、Yabuuchi T、Hayashi H.
    • Organizer
      World Conference on Regenerative Medicine
    • Place of Presentation
      Leipzig, Germany
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Effect of leucine for bone generation in porous hydroxyapatite scaffolds2013

    • Author(s)
      Kakigi H、Yoshikawa M、Hayashi H.
    • Organizer
      World Conference on Regenerative Medicine
    • Place of Presentation
      Leipzig, Germany
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Amino acid coating on a porous scaffold for osteogenesis in the pores by bone marrow cells2013

    • Author(s)
      Yabuuchi T、Yoshikawa M、Hayashi H.
    • Organizer
      World Conference on Regenerative Medicine
    • Place of Presentation
      Leipzig, Germany
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Effects of amino acids for osteogenesis by bone marrow cells2013

    • Author(s)
      Yabuuchi T、Yoshikawa M、Hayashi H.
    • Organizer
      13th International Conference of the European Ceramic Society
    • Place of Presentation
      Limoges, France
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Porous hydroxyapatite scaffold with a hollow center for bone formation in vivo2013

    • Author(s)
      Yoshikawa M、Kakigi H、Tsuji N、Yabuuchi T、Hayashi H.
    • Organizer
      13th International Conference of the European Ceramic Society
    • Place of Presentation
      Limoges, France
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Laminin for hard tissue formation in porous hydroxyapatite scaffolds by bone marrow cells in vivo2013

    • Author(s)
      Hideyuki Kakigi, Masataka Yoshikawa, Yasunori Shimomura, Norimasa Tsuji, Hiroyuki Hayashi
    • Organizer
      13th International Conference of the European Ceramic Society
    • Place of Presentation
      Limoges, France
    • Related Report
      2013 Annual Research Report
  • [Presentation] Hard tissue formation by bone marrow cells in hybrid scaffold with tryptophan or lysine coating sponge / hydroxyapatite2013

    • Author(s)
      Masataka Yoshikawa, Hideyuki Kakigi, Takayoshi Yabuuchi, Norimasa Tsuji, Hiroyuki Hayashi
    • Organizer
      International Bone-Tissue-Engneering Congress 2013
    • Place of Presentation
      Singapore
    • Related Report
      2013 Annual Research Report
  • [Presentation] Effects of two amino acids for hard tissue formation by bone marrow stem cells in vitro2013

    • Author(s)
      Hideyuki Kakigi, Masataka Yoshikawa, Norimasa Tsuji, Hiroyuki Hayashi
    • Organizer
      International Bone-Tissue-Engneering Congress 2013
    • Place of Presentation
      Singapore
    • Related Report
      2013 Annual Research Report
  • [Presentation] Hybrid scaffolds composed of amino acid coated sponge and hydroxyapatite for hard tissue formation by bone marrow cells2013

    • Author(s)
      Takayoshi Yabuuchi, Masataka Yoshikawa, Hideyuki Kakigi, Hiroyuki Hayashi
    • Organizer
      International Bone-Tissue-Engneering Congress 2013
    • Place of Presentation
      Singapore
    • Related Report
      2013 Annual Research Report
  • [Presentation] アルギン酸ナトリウム/リン酸カルシウム複合スポンジ状担体での骨髄幹細胞による硬組織形成2012

    • Author(s)
      薮内崇督、柿木栄幸、辻則正、好川正孝、林宏行
    • Organizer
      日本歯科保存学会2012年度春季学術大会(136回)
    • Place of Presentation
      宜野湾市
    • Year and Date
      2012-06-29
    • Related Report
      2013 Final Research Report
  • [Presentation] Osteogenesis by dextran coating on and among fibers of poly-vinyl formal sponge2012

    • Author(s)
      Yoshikawa M、Shimomura Y、Tsuji N、Yabuuchi T、Kakigi H、Hayashi H.
    • Organizer
      2012 Sino-Japan Dental Conference日中歯科医学大会2012
    • Place of Presentation
      成都、中華人民共和国
    • Year and Date
      2012-04-27
    • Related Report
      2013 Final Research Report
  • [Presentation] Osteogenesis in porous hydroxyapatite scaffolds with bone marrow cells in alginic acid gel2012

    • Author(s)
      Yoshikawa M、Kakigi H、Shimomura Y、Tsuji N、Hayashi H.
    • Organizer
      International Congress on Stem Cells and Tissue Formation
    • Place of Presentation
      Dresden、Germany
    • Related Report
      2013 Final Research Report
  • [Presentation] Hard tissue formation in a hybrid alginate /tri-calcium phosphate sponge in vitro2012

    • Author(s)
      Kakigi H、Yoshikawa M、Hayashi H.
    • Organizer
      International Congress on Stem Cells and Tissue Formation
    • Place of Presentation
      Dresden、Germany
    • Related Report
      2013 Final Research Report
  • [Presentation] Hard tissue formation in a novel hybrid alginate/calcium phosphate sponge in vitro2012

    • Author(s)
      Kakigi H、Yoshikawa M、Hayashi H.
    • Organizer
      15th European conference on composite materials
    • Place of Presentation
      Venice、Italy
    • Related Report
      2013 Final Research Report
  • [Presentation] Hard tissue formation in sponges by bone marrow cells suspended in an alginate gel2012

    • Author(s)
      Yoshikawa M、Kakigi H、Tsuji N、Hayashi H.
    • Organizer
      15th European conference on composite materials
    • Place of Presentation
      Venice、Italy
    • Related Report
      2013 Final Research Report
  • [Presentation] アルギン酸ナトリウム/リン酸カルシウム複合スポンジ状担体での骨髄幹細胞による硬組織形成2012

    • Author(s)
      薮内 崇督、柿木 栄幸、辻 則正、好川 正孝、林 宏行
    • Organizer
      日本歯科保存学会2012年度春季学術大会(第136回)
    • Place of Presentation
      沖縄県宜野湾市
    • Related Report
      2012 Research-status Report
  • [Presentation] Enhancement of bone generation by leucine coating in pores of hydroxyapatite scaffolds.2012

    • Author(s)
      Hayashi H, Yoshikawa M, Tsuji N, Shimomura Y, Kakigi H.
    • Organizer
      2012 Sino-Japan Dental Conference
    • Place of Presentation
      中華人民共和国 成都市
    • Related Report
      2012 Research-status Report
  • [Presentation] Osteogenesis by dextran coating on and among fibers of poly-vinyl formal sponge.2012

    • Author(s)
      Yoshikawa M, Shimomura Y, Tsuji N, Yabuuchi T, Kakigi H, Hayashi H.
    • Organizer
      2012 Sino-Japan Dental Conference
    • Place of Presentation
      中華人民共和国 成都市
    • Related Report
      2012 Research-status Report
  • [Presentation] Effect of a hollow center in porous hydroxyapatite scaffold for bone formation with bone marrow cells.2012

    • Author(s)
      Tsuji N, Yoshikawa M, Shimomura Y, Yabuuchi T, Hayashi H.
    • Organizer
      2012 Sino-Japan Dental Conference
    • Place of Presentation
      中華人民共和国 成都市
    • Related Report
      2012 Research-status Report
  • [Presentation] Hard tissue formation in a novel hybrid alginate/calcium phosphate sponge in vitro2012

    • Author(s)
      Kakigi H, Yoshikawa M, Hayashi H.
    • Organizer
      15th European Conference on Composite Materials - ECCM15
    • Place of Presentation
      Italy, Venice
    • Related Report
      2012 Research-status Report
  • [Presentation] Hard tissue formation in sponges by bone marrow cells suspended in an alginate gel2012

    • Author(s)
      Yoshikawa M, Kakigi H, Shimomura Y, Tsuji N, Hayashi H.
    • Organizer
      15th European Conference on Composite Materials - ECCM15
    • Place of Presentation
      Italy, Venice
    • Related Report
      2012 Research-status Report
  • [Presentation] Osteogenesis in porous hydroxyapatite scaffolds with bone marrow cells in alginic acid gel2012

    • Author(s)
      Yoshikawa M, Kakigi H, Shimomura Y, Tsuji N, Hayashi H.
    • Organizer
      4th International Congress on Stem Cells and Tissue Formation
    • Place of Presentation
      Germany, Dresden
    • Related Report
      2012 Research-status Report
  • [Presentation] Hard tissue formation in a hybrid alginate/tri-calcium phospate sponge in vitro2012

    • Author(s)
      Kakigi H, Yoshikawa M, Hayashi H.
    • Organizer
      4th International Congress on Stem Cells and Tissue Formation
    • Place of Presentation
      Germany, Dresden
    • Related Report
      2012 Research-status Report
  • [Presentation] 新規ハイブリッドアルギン酸ナトリウム/リン酸三カルシウムスポンジ状担体の骨髄幹細胞による骨形成能2011

    • Author(s)
      柿木栄幸、好川正孝、林宏行
    • Organizer
      日本歯科保存学会2011年度秋季学術大会(第135回)
    • Place of Presentation
      大阪市
    • Year and Date
      2011-10-21
    • Related Report
      2013 Final Research Report
  • [Presentation] Effect of laminin for osteogenesis using bone marrow cells in vivo and in vitro2011

    • Author(s)
      Yoshikawa M、Tsuji N、Kakigi H、Hayashi H.
    • Organizer
      International Bone -Tissue -Engneering Congress 2011
    • Place of Presentation
      Hannover、Germany
    • Related Report
      2013 Final Research Report
  • [Presentation] Property for hard tissue formation in sponge scaffolds by bone marrow cells with alginate2011

    • Author(s)
      Yoshikawa M、Kakigi H、Hayashi H.
    • Organizer
      World Conference on Regenerative Medicine
    • Place of Presentation
      Leipzig, Germany
    • Related Report
      2013 Final Research Report
  • [Presentation] Osteogenesis by bone marrow cells in novel hybrid alginate/calcium phosphate sponge scaffold2011

    • Author(s)
      Kakigi H、Yoshikawa M、Hayashi H.
    • Organizer
      World Conference on Regenerative Medicine
    • Place of Presentation
      Leipzig, Germany
    • Related Report
      2013 Final Research Report
  • [Presentation] Osteogenesis by bone marrow cells in novel hybrid alginate/calcium phosphate sponge scaffold2011

    • Author(s)
      Hideyuki Kakigi,Masataka Yoshikawa,Hiroyuki Hayashi
    • Organizer
      World Conference on Regenerative Medicine
    • Place of Presentation
      Leipzig. Germany
    • Related Report
      2011 Research-status Report
  • [Presentation] Property for hard tissue formation in sponge scaffolds by bone marrow cells with alginate in vitro2011

    • Author(s)
      Masataka Yoshikawa, Hideyuki Kakigi, Hiroyuki Hayashi
    • Organizer
      World Conference on Regenerative Medicine
    • Place of Presentation
      Leipzig. Germany
    • Related Report
      2011 Research-status Report
  • [Presentation] Effect of laminin for osteogenesis using bone marrow cells in vivo and in vitro2011

    • Author(s)
      Masataka Yoshikawa, Norimasa Tsuji, Hideyuki Kakigi, Hiroyuki Hayashi
    • Organizer
      International Bone-Tissue-Engineering Congress
    • Place of Presentation
      Hannover, Germany
    • Related Report
      2011 Research-status Report
  • [Presentation] ハイブリッドアルギン酸ナトリウム/リン酸三カルシウム多孔質担体内での骨髄細胞による硬組織形成2011

    • Author(s)
      柿木栄幸、好川正孝、林 宏行
    • Organizer
      第9回日本再生歯科医学会学術大会
    • Place of Presentation
      大阪市
    • Related Report
      2011 Research-status Report
  • [Presentation] 骨髄幹細胞による硬組織形成に及ぼすアミノ酸の効果

    • Author(s)
      柿木栄幸、 好川正孝、 辻 則正、 林 宏行
    • Organizer
      日本歯科保存学会2013年度秋季学術大会(139回)
    • Place of Presentation
      秋田市
    • Related Report
      2013 Annual Research Report
  • [Presentation] アミノ酸コーティングがスポンジ状担体内での硬組織形成促進に及ぼす影響

    • Author(s)
      藪内崇督, 好川正孝, 林 宏行
    • Organizer
      日本歯科保存学会2013年度秋季学術大会(139回)
    • Place of Presentation
      秋田市
    • Related Report
      2013 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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