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Bone formation of stressed cells under bio-mimetic condition

Research Project

Project/Area Number 23592824
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Prosthetic dentistry
Research InstitutionHokkaido University

Principal Investigator

IIDA Shunji  北海道大学, 大学病院, 講師 (30281827)

Co-Investigator(Kenkyū-buntansha) AKAZAWA Toshiyuki  北海道立総合研究機構, 産業技術研究本部, 研究主幹 (80469692)
YOKOYAMA Atsuro  北海道大学, 大学院歯学研究科, 教授 (20210627)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords骨形成 / 骨芽細胞 / メカニカルストレス / ハイドロキシアパタイト / コラーゲン / 骨芽細胞様細胞
Research Abstract

As a preliminary experiment, we investigated the effect of ultrasonic treatment for hydroxyapatite ceramics to the cell adhesion. Due to ultrasonic treatment at the surface of hydroxyapatite ceramics, a plenty of micro pores and micro cracks were observed and the increasing cell numbers raised the ability of cell adhesion to the ceramics.
Osteoblastic cell culturing under the biomimetic condition caused cells extended the process three dimensionally on collagen gel. And Cells increased osteocalcin expression against mechanical stress by hydroxyl apatite block.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (15 results)

All 2013 2012 2011 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (12 results)

  • [Journal Article] Surface design and water vapor-adsorption characteristics of biomimetic2013

    • Author(s)
      Akazawa T, Murata M, Iida S, 他7名
    • Journal Title

      Key Engeering

      Volume: 529 Pages: 430-435

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Surface Design and Water Vapor- Adsorption Characteristics of Biomimetic2013

    • Author(s)
      T. Akazawa, M. Murata, S. Iida, et al
    • Journal Title

      Key Engineering

      Volume: 529-530 Pages: 430-435

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Surface structure and biocompatibility of demineralized dentin matrix granules soaked in a stimulated body fluid2012

    • Author(s)
      T Akazawa, M Murata, S Iida, 他10名
    • Journal Title

      Applied Surface Science

      Volume: No.262 Pages: 51-55

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] Materials design and cell activity of apatite/collagen composites on Ti substrates by the electrospinings2013

    • Author(s)
      Akazawa T, Murata M, Minamida M, Iida S, et al.
    • Organizer
      アジアバイオセラミックスシンポジウム
    • Place of Presentation
      京都大学(京都市)
    • Year and Date
      2013-12-04
    • Related Report
      2013 Final Research Report
  • [Presentation] エレクトロスピニング法による細胞培養用アパタイト/チタン複合基材の作製2013

    • Author(s)
      赤澤敏之、村田勝、南田康人、飯田俊二, ら
    • Organizer
      日本セラミックス協会第26回秋季シンポジウム
    • Place of Presentation
      信州大学(長野市)
    • Year and Date
      2013-09-04
    • Related Report
      2013 Final Research Report
  • [Presentation] 超音波部分溶解・析出処理による一方向性多孔体人工骨の骨誘導促進効果2013

    • Author(s)
      柏崎晴彦、原田尚樹、赤澤敏之、飯田俊二, ら
    • Organizer
      第25回北海道骨粗鬆症研究会
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2013-02-09
    • Related Report
      2013 Final Research Report
  • [Presentation] エレクトロスピニング法による細胞培養用アパタイト/チタン複合基材の作製2013

    • Author(s)
      赤澤敏之、村田勝、南田康人、大久保直登、今野克美、野村隆文、飯田俊二、伊東学、宮崎聡、中島武彦
    • Organizer
      日本セラミックス協会第26回秋季シンポジウム
    • Place of Presentation
      信州大学長野キャンパス(長野市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Materials desigh and cell activity of apatite/collagen composites on Ti substrates by the electrospinning2013

    • Author(s)
      T Akazawa M Murata Y Minamida N Okubo K Konno T Nomura S Iida M Ito S Miyazaki T Nakajima
    • Organizer
      アジアバイオセラミックスシンポジウム
    • Place of Presentation
      京都大学百周年時計台記念館(京都市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Surface design and water vapor-adsorption characteristics of biomimetic2012

    • Author(s)
      Akazawa T, Murata M, Nomura T, Shingyo K, Sakai K, Iida S, et al.
    • Organizer
      Bioceramics 24
    • Place of Presentation
      九州大学(福岡市)
    • Year and Date
      2012-10-21
    • Related Report
      2013 Final Research Report
  • [Presentation] 海洋資源由来吸収生体模倣複合材料の創薬と応用2012

    • Author(s)
      赤澤敏之、執行達弘、野村隆文、山岸暢、稲野浩行、飯田俊二, ら
    • Organizer
      日本セラミックス協会2012年度基礎科学部会セミナー・北海道地区セミナー
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2012-07-27
    • Related Report
      2013 Final Research Report
  • [Presentation] Surface design and water vapor- adsorption characteristics of biomimetic2012

    • Author(s)
      T. Akazawa, M. Murata, T. Nomura, T. Shigyo, K. Sakai, S. Iida, et al
    • Organizer
      Bioceramics 24
    • Place of Presentation
      九州大学(福岡)
    • Related Report
      2012 Research-status Report
  • [Presentation] 水酸アパタイトセラミックスの細胞接着特性に及ぼす超音波溶解処理の影響2011

    • Author(s)
      飯田俊二、横山敦郎、原田尚樹、柏崎晴彦、赤澤敏之, ら
    • Organizer
      日本セラミックス協会第24回秋季シンポジウム
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2011-09-08
    • Related Report
      2013 Final Research Report
  • [Presentation] 超音波部分溶解・析出処理による一方向性多孔体人工骨の骨誘導促進効果

    • Author(s)
      柏崎晴彦、原田尚樹、赤澤敏之、飯田俊二、Kabir Md Arafat, 村田勝
    • Organizer
      北海道骨粗鬆症研究会
    • Place of Presentation
      北海道大学(札幌)
    • Related Report
      2012 Research-status Report
  • [Presentation] 海洋資源由来吸収性生体模倣複合材料の創薬と応用

    • Author(s)
      赤澤敏之, 執行達弘, 野村隆文, 山岸暢, 稲野浩行、飯田俊二他
    • Organizer
      日本セラミックス協会
    • Place of Presentation
      北海道大学(札幌)
    • Related Report
      2012 Research-status Report
  • [Presentation] 水酸アパタイトセラミックスの細胞接着特性に及ぼす超音波溶解処理の影響

    • Author(s)
      飯田俊二、横山敦郎、原田尚樹、柏崎晴彦、赤澤敏之、村田勝、伊東学、中島武彦
    • Organizer
      日本セラミックス協会
    • Place of Presentation
      北海道大学(札幌)
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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