| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
In this project we try to provide a micropipette for the in vivo patch clamp technique. Unfortunately, few probes were obtained to allow forming the high resistance seal (giga-ohm seal). The other device was also needed to reach the objective neurons of the in vivo specimen. These micropipettes, however, allowed us performing extracellular recording. Neuronal activities responding mechanical stimulation to the oro-facial region were extracellularly recorded from the ventral posteromedial nucleus of the rabbit thalamus. Ninety percent of recorded neurons responded to mechanical stimuli associated with occluding and chewing and also related to the mental activity. These results suggested that sensory information from oro-facial region during occlusion and mastication influences on the level of consciousness.
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