| Project/Area Number |
23592864
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Prosthetic dentistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
AKIRA Ishisaki 岩手医科大学, 歯学部, 教授 (20356439)
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| Co-Investigator(Renkei-kenkyūsha) |
SHINJI Kuroda (50323689)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 歯科 / 骨組織再生 / 遺伝子導入 / 骨組織 / 再生 / 遺伝子 / 動物実験 / ＢＭＰ / 骨欠損 / 組織再生 / 骨 |
|---|
| Research Abstract |
We have tried to establish a method of minimum invasive bone augmentation with nano-devices containing DNAs. Our preliminary experiment showed that direct in vivo gene transfer technique successfully introduced plasmid DNA of green fluorescence protein into cells of calvarial tissue. However, BMP-2 plasmid did not show positive effects for bone tissue regeneration in rat calvarial bone defect models. On the other hand, in another experiments to search the candidates of gene delivery, osteogenic differentiation of UE7T-13 cells, a bone marrow-derived hMSC line, was markedly enhanced by PDGF, although PDGF alone did not induce differentiation. These results suggest that the enhancement of TGF-beta(TGF)-inducedinduced osteogenic differentiation by PDGF-induced PI3K/Akt-mediated signaling depends on TGF-induced MEK activity. Thus, PDGF positively modulates the TGFinduced osteogenic differentiation of hMSCs through synergistic crosstalk between MEK- and PI3K/Akt-mediated signaling.
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