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Establishment of minimum invasive bone augmentation technique with nano-devices containing DNAs

Research Project

Project/Area Number 23592864
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Prosthetic dentistry
Research InstitutionIwate Medical University

Principal Investigator

KONDO HISATOMO  岩手医科大学, 歯学部, 教授 (70343150)

Co-Investigator(Kenkyū-buntansha) AKIRA Ishisaki  岩手医科大学, 歯学部, 教授 (20356439)
Co-Investigator(Renkei-kenkyūsha) SHINJI Kuroda   (50323689)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords歯科 / 骨組織再生 / 遺伝子導入 / 骨組織 / 再生 / 遺伝子 / 動物実験 / BMP / 骨欠損 / 組織再生 / 骨
Research Abstract

We have tried to establish a method of minimum invasive bone augmentation with nano-devices containing DNAs. Our preliminary experiment showed that direct in vivo gene transfer technique successfully introduced plasmid DNA of green fluorescence protein into cells of calvarial tissue. However, BMP-2 plasmid did not show positive effects for bone tissue regeneration in rat calvarial bone defect models. On the other hand, in another experiments to search the candidates of gene delivery, osteogenic differentiation of UE7T-13 cells, a bone marrow-derived hMSC line, was markedly enhanced by PDGF, although PDGF alone did not induce differentiation. These results suggest that the enhancement of TGF-beta(TGF)-inducedinduced osteogenic differentiation by PDGF-induced PI3K/Akt-mediated signaling depends on TGF-induced MEK activity. Thus, PDGF positively modulates the TGFinduced osteogenic differentiation of hMSCs through synergistic crosstalk between MEK- and PI3K/Akt-mediated signaling.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (26 results)

All 2014 2013 2012 2011

All Journal Article (8 results) (of which Peer Reviewed: 6 results,  Open Access: 1 results) Presentation (18 results)

  • [Journal Article] PDGF-induced PI3K-mediated signal enhances TGF-βinduced osteogenic differentiation of human mesenchymal stem cells in the TGF-β-activated MEK-dependent manner2014

    • Author(s)
      Jun Yokota, Naoyuki Chosa, Shunsuke Sawada, Naoto Okubo, Noriko Takahashi, Tomokazu Hasegawa, Hisatomo Kondo, Akira Ishisaki
    • Journal Title

      International Journal of Molecular Medicine

      Volume: 33 Issue: 3 Pages: 534-542

    • DOI

      10.3892/ijmm.2013.1606

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of apatite particle size contained in two apatite/collagen composites on osteogenic differentiation profile in osteoblastic cells.2013

    • Author(s)
      Hatakeyama W., Taira M., Chosa N., Kihara H., Ishisaki A., Kondo H.
    • Journal Title

      International Journal of Molecular Medicine

      Volume: 32 Issue: 6 Pages: 1255-1261

    • DOI

      10.3892/ijmm.2013.1516

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Subcutaneous Tissue Reactions Against Nano-apatite Collagen Composites2012

    • Author(s)
      W HATAKEYAMA, M TAIRA, H KIHARA, M SASAKI, S KIMURA, H KONDO
    • Journal Title

      Nano Biomedicine

      Volume: 4(2) Pages: 118-124

    • NAID

      130004679846

    • URL

      http://www.nanobio.jp/n4-2-7e.html

    • Related Report
      2013 Final Research Report
  • [Journal Article] Quantitative Analyses of Osteogenic-differentiation-related Gene Expressions in Human Osteoblast-like Cells (SaOS-2)2012

    • Author(s)
      M TAIRA, W HATAKEYAMA, H KIHARA, H KONDO, K UEDA, T NARUSHIMA
    • Journal Title

      Journal of Oral Tissue Engineering

      Volume: 10(1) Pages: 34-41

    • URL

      http://mol.medicalonline.jp/library/journal/download?GoodsID=di3ortis/2012/001001/004&name=0034-0041e&UserID=202.244.196.214

    • Related Report
      2013 Final Research Report
  • [Journal Article] Subcutaneous Tissue Reactions Against Nano-apatite Collagen Composites2012

    • Author(s)
      Wataru HATAKEYAMA, Masayuki TAIRA, Hidemichi KIHARA, Minoru SASAKI, Shigenobu KIMURA Hisatomo KONDO
    • Journal Title

      Nano Biomedicine

      Volume: 4(2) Pages: 118-124

    • NAID

      130004679846

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Quantitative Analyses of Osteogenic-differentiation-related Gene Expressions in Human Osteoblast-like Cells (SaOS-2) Cultured on Hydroxyapatite and Titanium2012

    • Author(s)
      Masayuki TAIRA, Wataru HATAKEYAMA, Hidemichi KIHARA, Hisatomo KONDO, Kyosuke UEDA and Takayuki NARUSHIMA
    • Journal Title

      Journal of Oral Tissue Engineering

      Volume: 10(1) Pages: 34-41

    • NAID

      130004941076

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cholesteryl group- and acryloylgroup-bearing pullulan nanogel to deliver BMP2 and FGF18 for bone tissue engineering2012

    • Author(s)
      Fujioka-Kobayashi M, Ota MS, Shimoda A, Nakahama K, Akiyoshi K, Miyamoto Y, Iseki S
    • Journal Title

      Biomaterials

      Volume: Vol.3、No.30 Issue: 2 Pages: 7613-20

    • DOI

      10.1111/j.1600-0765.2011.01419.x

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Implantation of green tea catechin α-tricalcium phosphate combination enhances bone repair in rat skull defects.2011

    • Author(s)
      Rodriguez R, Kondo H, Nyan M, Hao J, Miyahara T, Ohya K, Kasugai S
    • Journal Title

      Journal of Biomedical Material Research Part B

      Volume: 98B Pages: 263-271

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] GFPマウス骨髄由来間葉系幹細胞株の骨分化におけるBMP-2の影響2014

    • Author(s)
      五十嵐靖之,横田潤,佐藤友秀,原淳,近藤尚知
    • Organizer
      第44回日本口腔インプラント学会学術大会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] アパタイト/コラーゲン複合体中のアパタイト粒子の違いが骨芽細胞様細胞の骨系分化挙動に及ぼす影響評価2013

    • Author(s)
      畠山航, 平雅之, 鬼原英道, 高藤恭子, 近藤尚知
    • Organizer
      第43回日本口腔インプラント学会学術大会
    • Place of Presentation
      福岡
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] TGF-βとIGF-I, PDGFまたはVEGFの間葉系幹細胞に対する骨分化促進効果2013

    • Author(s)
      横田潤, 鬼原英道, 三浦真吾, 高藤恭子, 近藤尚知
    • Organizer
      第43回日本口腔インプラント学会学術大会
    • Place of Presentation
      福岡
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 細胞分離回収用磁性マイクロビーズに関する研究2013

    • Author(s)
      畠山航, 平雅之、鬼原英道, 近藤尚知
    • Organizer
      日本口腔インプラント学会東北・北海道支部総会・学術大会
    • Place of Presentation
      弘前
    • Related Report
      2013 Final Research Report
  • [Presentation] Combination of TGF-βand other cytokines synergistically enhances osteogenic differentiation of mesenchymal stem cells2013

    • Author(s)
      J YOKOTA, HI KIHARA, K TAKAFUJI, H KONDO, A ISHISAKI, T KOBAYASHI
    • Organizer
      22nd Annual Scientific Meeting of European Association for Osseointegration
    • Place of Presentation
      Dublin, Ireland
    • Related Report
      2013 Final Research Report
  • [Presentation] Bone regeneration of rat critical size calvarial defect with large-size fully inter-connected porous apatite/collagen composite2013

    • Author(s)
      W HATAKEYAMA, H KIHARA, K TAKAFUJI, H KONDO
    • Organizer
      22nd Annual Scientific Meeting of European Association for Osseointegration
    • Place of Presentation
      Dublin, Ireland
    • Related Report
      2013 Final Research Report
  • [Presentation] 細胞分離回収用磁性マイクロビーズに関する研究2013

    • Author(s)
      畠山航,平雅之、鬼原英道,近藤尚知
    • Organizer
      日本口腔インプラント学会 東北・北海道支部学術大会
    • Place of Presentation
      弘前
    • Related Report
      2013 Annual Research Report
  • [Presentation] Combination of TGF-β and Other Cytokines Synergistically Enhances Osteogenic Differentiation of Mesenchymal Stem Cells2013

    • Author(s)
      Jun YOKOTA, Hidemichi KIHARA, Kyoko TAKAHUJI, Hisatomo KONDO
    • Organizer
      EAO Annual Scientific Congress 2013
    • Place of Presentation
      Dublin, Ireland
    • Related Report
      2013 Annual Research Report
  • [Presentation] ナノアパタイト粒子配合医療用コラーゲンを用いたラット頭蓋骨欠損部における骨再生の試み2012

    • Author(s)
      畠山航, 松本知生, 丸尾勝一郎, 鬼原英道, 近藤尚知
    • Organizer
      第42回公益社団法人日本口腔インプラント学会学術大会
    • Place of Presentation
      大阪市
    • Related Report
      2013 Final Research Report
  • [Presentation] 多血小板血漿由来主要サイトカインが間葉系幹細胞の骨分化に及ぼす影響2012

    • Author(s)
      横田潤, 髙藤恭子, 丸尾勝一郎, 鬼原英道, 近藤尚知
    • Organizer
      第42回公益社団法人日本口腔インプラント学会・学術大会
    • Place of Presentation
      大阪市
    • Related Report
      2013 Final Research Report
  • [Presentation] Combination of TGF-β and other cytokines such as IGF-I, PDGF, or VEGF enhances osteogenic differentiation of mesenchymal stem cells.2012

    • Author(s)
      横田潤,帖佐直幸,大久保直登,衣斐美歩,鬼原秀道,丸尾勝一郎,客本斎子,加茂政晴,近藤尚知,石崎明
    • Organizer
      第35回 日本分子生物学会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡
    • Related Report
      2012 Research-status Report
  • [Presentation] 多孔質アパタイト粒子コラーゲン複合体によるラット頭蓋骨クリティカルサイズ骨欠損部における骨再生の試み2012

    • Author(s)
      畠山 航,平 雅之,鬼原 英道,近藤 尚知
    • Organizer
      第60回日本歯科理工学会
    • Place of Presentation
      九州大学医学部百年講堂
    • Related Report
      2012 Research-status Report
  • [Presentation] 多血小板血漿由来主要サイトカインが間葉系幹細胞の骨分化に及ぼす影響2012

    • Author(s)
      横田 潤, 鬼原 英道, 丸尾 勝一郎, 髙藤 恭子, 近藤 尚知
    • Organizer
      第42回日本口腔インプラント学会
    • Place of Presentation
      大阪国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] ナノアパタイト粒子配合医療用コラーゲンを用いたラット頭蓋骨欠損部における骨再生の試み2012

    • Author(s)
      畠山航, 松本知生, 丸尾 勝一郎, 鬼原 英道, 近藤 尚知
    • Organizer
      第42回日本口腔インプラント学会
    • Place of Presentation
      大阪国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] 義歯床によるオトガイ孔の圧迫に対してインプラントオーバーデンチャーによる無歯顎補綴が奏功した一症例2012

    • Author(s)
      古屋純一, 佐々木健, 玉田泰嗣, 小林琢也, 近藤尚知
    • Organizer
      第42回日本口腔インプラント学会
    • Place of Presentation
      大阪国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] 骨移植を行わない上顎洞底挙上術の1症例2012

    • Author(s)
      横田潤,畠山航,朴沢一成,高藤恭子,近藤尚知
    • Organizer
      平成24年度日本補綴歯科学会東北北海道支部会
    • Place of Presentation
      東北大学艮陵会館
    • Related Report
      2012 Research-status Report
  • [Presentation] オトガイ孔の3次元的位置に関する研究2011

    • Author(s)
      佐々木健,古谷純一,玉田泰嗣,澤田 愛,横瀬隆夫,大久保卓也,織田展輔,近藤尚知,
    • Organizer
      日本歯科補綴学会第120回記念学術大会
    • Place of Presentation
      広島
    • Related Report
      2011 Research-status Report
  • [Presentation] 顎骨再建症例におけるインプラント喪失原因に関する臨床的 検討2011

    • Author(s)
      楠本 雄生 、立川 敬子 、宗像 源博 、近藤 尚知 、春日井 昇平
    • Organizer
      第41回日本口腔インプラント学会学術大会
    • Place of Presentation
      名古屋
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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