Production and application of a dental hypoplasia mouse model using a specific organization and a cell disruption system
| Project/Area Number |
23592892
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Kagoshima University |
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Principal Investigator |
MATSUMOTO Yuko 鹿児島大学, 医学部・歯学部附属病院, 助教 (20315443)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Masahiro 鹿児島大学, 医用ミニブタ先端開発研究センター, 教授 (30287099)
SAITOH Issei 新潟大学, 医歯学総合研究科, 准教授 (90404540)
YAMASAKI Youichi 鹿児島大学, 医歯学総合研究科, 教授 (30200645)
NOGUCHI Hirofumi 独立行政法人国立病院機構, 千葉東病院臨床研究センター, 研究員 (50378733)
SAITOH Yoko 新潟大学, 医歯学総合研究科, 助教 (30404487)
INADA Emi 鹿児島大学, 医学部・歯学部附属病院, 除教 (30448568)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 歯形成 / ameloblast / odontoblast / Tgマウス / odontblast |
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| Research Abstract |
We produced a plasmid that specifically expressed a DT-A gene in cells responsible for tooth development, and a construct (pARIC), which connected the red fluorescent gene (RFP) and the Cre gene downstream from amelogenin promoter. In order to check the operation of the plasmid, pARIC and pCETZ-17 were poured into the palate of a two-week-old mouse, and the gene was introduced in vivo by electroporation. The result showed the expression of the lacZ gene indicating that the expression of downstream lacZ gene was the result of the expression of the Cre gene by amelogenin promoter. Moreover, this removed "EGFP cDNA+stopper" inserted by loxP in pCETZ-17.
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Report
(4 results)
Research Products
(41 results)
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[Journal Article] Comparison of new preservation solution, HN-1, and University of Wisconsin solution in pancreas preservation for porcine islet isolation.2013
Author(s)
Akihiro K, Noguchi H, Kuise T, Nakatsuka A, Hirota D, Kataoka H, Kawai T, Inoue K, Imagawa N, Saitoh I, Noguchi Y, Watanabe M, Wada J, Fujiwara T
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Journal Title
Cell Med.
Volume: 6
Pages: 3-8
DOI
Related Report
Peer Reviewed
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[Journal Article] Targeted transgenesis through pronuclear injection of improved vectors into in vitro fertilized eggs2012
Author(s)
Ohtsuka, M., Miura, H., Nakaoka, H., Kimura, M., Sato, M., Inoko, H.
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Journal Title
Transgenic Res.
Volume: 21:1
Pages: 225-226
DOI
Related Report
Peer Reviewed
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[Journal Article] CD81 and CD9 work independently as extracellular components upon fusion of sperm and oocyte.2012
Author(s)
Ohnami N, Nakamura A, Miyado M, Sato M, Kawano N, Yoshida K, Harada Y, Takezawa Y, Kanai S, Ono C, Takahashi Y, Kimura K, Shida T, Miyado K, Umezawa A
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Journal Title
Biol Open.
Volume: 1(7)
Pages: 640-647
DOI
Related Report
Peer Reviewed
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[Presentation] ヒト乳歯歯髄由来iPS細胞樹立におけるフィーダー細胞選択の重要性2013
Author(s)
村上智哉, 齊藤一誠, 稲田絵美, 岩瀬陽子, 長谷川大子, 窪田直子, 松本祐子, 大島邦子,岡暁子, 山崎要一, 早崎治明
Organizer
第51回日本小児歯科学会
Place of Presentation
岐阜
Related Report
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[Presentation] ヒト乳歯歯髄由来iPS細胞樹立におけるフィーダー細胞選択の重要性2013
Author(s)
村上智哉, 齊藤一誠, 稲田絵美, 岩瀬陽子, 長谷川大子, 窪田直子, 松本祐子, 大島邦子, 岡 暁子, 山崎要一, 早崎治明
Organizer
第５１回日本小児歯科学会
Place of Presentation
岐阜県
Related Report
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