Investigation of new biological functions of neuropeptide and thrapeutic application for oral mucosal diseases

• Project/Area Number: 23592924
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Hiroshima University
• Principal Investigator: KOUJI Ohta 広島大学, 大学病院, 助教 (20335681)
• Co-Investigator(Kenkyū-buntansha): SHIGEISHI Hideo 広島大学, 医歯(薬)学総合研究科, 助教 (90397943) ; TAKECHI Masaaki 広島大学, 医歯(薬)学総合研究科, 准教授 (00304535) ; KAMATA Nobuyuki 広島大学, 医歯(薬)学総合研究科, 教授 (70242211)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 神経ペプチド / 口腔粘膜細胞 / 炎症性疾患 / 口腔粘膜上皮細胞 / 炎症性口腔粘膜疾患 / 抗菌作用 / 抗炎症作用

Research Abstract

Neuropeptide including ghrelin and VIP is thought to have the role in regulating the function in immune system in oral mucosa as well as functioning as neurotransmitters . In this study, we showed expression of ghrelin and VIP constitutively expressed in oral keratinocytes. Ghrelin has ant-bactericidal effects against E. coli and P. aeruginosa. Furthermore, ghrelin inhibited the production of IL-8 from inflammatory cytokines in oral keratinocytes. On the other hands, vascular endothelial factor, VEGF was induced by VIP in oral keratinocytes, and VIP increased cell migration. In conclusion, we demonstrated that neuropeptide, ghrelin and VIP may function as an immune-modulation and wound healing factor in the oral mucosa.

Research Products

• [Journal Article] Itraconazole inhibits TNF-α-induced CXCL10 expression in oral fibroblasts (2014)
  • Author(s): Ohta K, Ishida Y, Fukui A, Nishi H, Naruse T, Takechi M, Kamata N
  • Journal Title: Oral Dis Volume: 21 Issue: 1 Pages: 106-112
  • DOI: 10.1111/odi.12226
  • Peer Reviewed
• [Journal Article] Expression of receptor for hyaluronan-mediated motility (RHAMM)in ossifying fibromas (2013)
  • Author(s): Hatano H, Ogawa I, Shigeishi H, Kudo Y, Ohta K, Higashikawa K, Takechi M, Takata T, Kamata N
  • Journal Title: Histol Histopathol. Histol Histopathol Volume: 28 Pages: 473-480
  • Peer Reviewed
• [Journal Article] Bone formation and osseointegration with titanium implant using granular- and block-type porous hydroxyapatite ceramics (IP-CHA) (2013)
  • Author(s): Minami M, Takechi M, Ohta K, Ohta A, Ninomiya Y, Takamoto M, Fukui A, Tada M, , Kamata N
  • Journal Title: Dent Mater J Volume: 32 Pages: 753-760
  • NAID: 10031191710
  • Peer Reviewed
• [Journal Article] Risk of bacterial contamination of bone harvesting devices used for autogenous bone graft in implant surgery. (2013)
  • Author(s): Takamoto M, Takechi M, Ohta K, Ninomiya Y, Ono S, Shigeishi H, Tada M, Kamata N
  • Journal Title: Head Face Med Volume: 9 Issue: 1 Pages: 3-3
  • DOI: 10.1186/1746-160x-9-3
  • Peer Reviewed
• [Journal Article] Interleukin-8 and CXCL10 expressions in oral keratinocytes and fibroblasts via Toll-like receptors (2013)
  • Author(s): Fukui A, Ohta K, Nishi H, Shigeishi H, Tobiume K, Takechi M, Kamata N
  • Journal Title: Microbiol Immunol Volume: 57 Pages: 198-206
  • NAID: 40019615142
  • Peer Reviewed
• [Journal Article] Clinical evaluation of novel interconnected porous hydroxyapatite ceramics (IP-CHA)in a maxillary sinus floor augmentation procedure (2012)
  • Author(s): Shigeishi H, Takechi M, Nishimura M, Takamoto M, Minami M, Ohta K, Kamata N
  • Journal Title: Dent Mater J Volume: 31 Pages: 54-60
  • NAID: 10030128207
  • Peer Reviewed
• [Journal Article] Establishment of mesenchymal cell line derived from human developing odontoma (2012)
  • Author(s): Hatano H, Kudo Y, Ogawa I, Shimasue H, Shigeishi H, Ohta K, Higashikawa K, Takechi M, Takata T, Kamata N
  • Journal Title: Oral Dis Volume: 18 Pages: 756-762
  • Peer Reviewed
• [Journal Article] 3-dimensional composite scaffolds consisting of apatite-PLGA-atelocollagen for bone tissue engineering (2012)
  • Author(s): Takechi M, Ohta K, Ninomiya Y, Tada M, Minami M, Takamoto M, Ohta A, Nakagawa T, Fukui A, Miyamoto Y, Kamata N
  • Journal Title: Dent Mater J Volume: 31 Pages: 465-471
  • NAID: 10030315479
  • Peer Reviewed
• [Journal Article] The antimicrobial activity of the appetite peptide hormone ghrelin (2012)
  • Author(s): Min C, Ohta K, Kajiya M, Zhu T, Sharma K, Shin J, Mawardi H, Howait M, Hirschfeld J, Bahammam L, Ichimonji I, Ganta S, Amiji M, Kawai T
  • Journal Title: Peptides Volume: 36 Pages: 151-156
  • Peer Reviewed
• [Journal Article] Expression and possible immune-regulatory function of ghrelin in oral epithelium (2011)
  • Author(s): Ohta K, Laborde NJ, Kajiya M, Shin J, Zhu T, Thondukolam AK, Min C, Kamata N, Karimbux NY, Stashenko P, Kawai T
  • Journal Title: J Dent Res Volume: 90 Pages: 1286-1292
  • Peer Reviewed
• [Journal Article] Toll-like receptor (TLR)expression and TLR-mediated IL-8 production by human submandibular gland epithelial cells
  • Author(s): Ohta K, Ishida Y, Fukui A, Mizuta K, Nishi H, Takechi M, Kamata N
  • Journal Title: Mol Med Rep Volume: (accepted)
  • Peer Reviewed
• [Presentation] 口腔粘膜上皮細胞におけるCandida albicansβ-glucanによる酸化ストレスとHemeoxygenase-1の発現誘導 (2013)
  • Author(s): 石田陽子, 太田耕司, 福井暁子, 西裕美, 奥井岳, 鳴瀬貴子, 武知正晃, 鎌田伸之
  • Organizer: 日本口腔組織培養学会設立50周年記念大会・総会
  • Place of Presentation: 東京
  • Year and Date: 2013-11-23
• [Presentation] 口腔粘膜上皮細胞におけるCandida albicansβ-glucanによるHemeoxygenase-1の発現誘導 (2013)
  • Author(s): 石田陽子, 太田耕司, 福井暁子, 西裕美, 奥井岳, 鳴瀬貴子, 武知正晃, 鎌田伸之
  • Organizer: 第58回日本口腔外科学会総会・学術大会
  • Place of Presentation: 福岡
  • Year and Date: 2013-10-12
• [Presentation] 口腔粘膜上皮細胞におけるCandida albicansβ-glucanによるHemeoxygenase-1の発現誘導と酸化ストレスに対する防御応答 (2013)
  • Author(s): 石田陽子, 太田耕司, 福井暁子, 西裕美, 奥井岳, 鳴瀬貴子, 武知正晃, 鎌田伸之
  • Organizer: 第23回口腔内科学会・第26回口腔診断学会合同学術大会
  • Place of Presentation: 東京
  • Year and Date: 2013-09-13
• [Presentation] Bacterial adhesion on osteoconductive-trated titanium implants (2013)
  • Author(s): Takamoto M, Takechi M, Ohta K, Tsuru K, Nikawa H, Ishikawa K, Kamata N
  • Organizer: 第46回広島大学歯学会総会
  • Place of Presentation: 広島
  • Year and Date: 2013-06-29
• [Presentation] 口腔粘膜上皮細胞におけるCandida albicansによるHemeoxygenase-1の発現誘導 (2013)
  • Author(s): 石田陽子, 太田耕司, 福井暁子, 西裕美, 奥井岳, 鳴瀬貴子, 武知正晃, 鎌田伸之
  • Organizer: 第67回NPO法人日本口腔科学会学術大会
  • Place of Presentation: 宇都宮
  • Year and Date: 2013-05-22
• [Presentation] 歯肉線維芽細胞における炎症性ケモカイン発現誘導に対するアゾール系抗真菌剤イトコナゾールの抑制作用の検討 (2012)
  • Author(s): 石田陽子, 太田耕司, 福井暁子, 西裕美, 武知正晃, 鎌田伸之
  • Organizer: 第49回日本口腔組織培養学会学術大会
  • Place of Presentation: 広島
  • Year and Date: 2012-11-17
• [Presentation] 歯肉線維芽細胞の炎症性ケモカイン発現誘導に対するイトコナゾールの抑制作用の検討 (2012)
  • Author(s): 太田耕司, 福井暁子, 西裕美, 石田陽子, 武知正晃, 鎌田伸之
  • Organizer: 第25回日本口腔粘膜学会・第22回日本口腔粘膜学会合同学術大会
  • Place of Presentation: 東京
  • Year and Date: 2012-09-20
• [Presentation] 口腔粘膜上皮細胞, 線維芽細胞における抗真菌剤イトコナゾールの抗炎症作用の検討 (2012)
  • Author(s): 太田耕司, 福井暁子, 西裕美, 石田陽子, 武知正晃, 鎌田伸之
  • Organizer: 第66回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 広島
  • Year and Date: 2012-05-17
• [Presentation] 口腔粘膜上皮細胞, 線維芽細胞におけるRIG-Iシグナルの機能解析 (2011)
  • Author(s): 福井暁子, 太田耕司, 重石英生, 西裕美, 武知正晃, 鎌田伸之
  • Organizer: 第56回日本口腔外科学会総会・学術大会
  • Place of Presentation: 大阪
  • Year and Date: 2011-10-22
• [Presentation] Expression and function of Retionic acid-inducible gene-I (RIG-I)in oral keratinocyte and fibroblast (2011)
  • Author(s): Fukui A, Ohta K, Shigeishi H, Nishi H, Takechi M, Kamata N
  • Organizer: 4^<th> Hiroshima Conference on Education and science in Dentistry
  • Place of Presentation: Hiroshima
  • Year and Date: 2011-10-10
• [Presentation] 口腔粘膜上皮細胞, 歯肉線維芽細胞におけるToll-like receptorを介した炎症性ケモカインの発現誘導 (2011)
  • Author(s): 太田耕司, 福井暁子, 西裕美, 重石英生, 武知正晃, 鎌田伸之
  • Organizer: 第21回日本口腔粘膜学会総会・学術大会
  • Place of Presentation: 鹿児島
  • Year and Date: 2011-09-24
• [Presentation] 口腔粘膜上皮細胞, 歯肉線維芽細胞におけるRIG-Iの発現とシグナル伝達経路の機能解析 (2011)
  • Author(s): 福井暁子, 太田耕司, 重石英生, 西裕美, 武知正晃, 鎌田伸之
  • Organizer: 第21回日本口腔粘膜学会総会・学術大会
  • Place of Presentation: 鹿児島
  • Year and Date: 2011-09-24
• [Presentation] 口腔粘膜上皮細胞, 歯肉線維芽細胞におけるRIG-Iを介した炎症性ケモカインの発現とシグナル伝達経路の解析 (2011)
  • Author(s): 福井暁子, 太田耕司, 重石英生, 西裕美, 武知正晃, 鎌田伸之
  • Organizer: 第65回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2011-04-21