Molecular analyses on the intercellular signaling relating to the bone destruction of ameloblastoma
Project/Area Number |
23592929
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Surgical dentistry
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Research Institution | Kagoshima University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
KISHIDA Shousei 鹿児島大学, 医歯(薬)学総合研究科, 教授 (50274064)
KIYONO Tohru 独立行政法人国立がんセンター, 部長 (10186356)
岸田 想子 鹿児島大学, 医歯(薬)学総合研究科, 助教 (40274089)
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Co-Investigator(Renkei-kenkyūsha) |
HIJIOKA Hiroshi 鹿児島大学, 医学部・歯学部附属病院, 講師 (70305150)
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Project Period (FY) |
2011 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
Keywords | 口腔外科一般 / エナメル上皮腫 / 歯原性腫瘍 / Wntシグナル / 歯原性上皮 / 骨浸潤 / 顎骨腫瘍 / Wnt / MMP |
Research Abstract |
Immortalized epithelial cell line derived from oral mucosa (MOE-1), as control, and immortalized ameloblastoma cell line derived from follicular-type ameloblastoma (AM-3) were established and their biological characteristics were compared to AM-1, previously established immortalized ameloblastoma cells derived from prexiform-type ameloblastoma. AM-1 and AM-3 demonstrated positive reaction to the epithelial markers and negative to the mesenchymal one maintaining the morphological characteristics of the epithelial cell. Expressions of MMP-2 and MMP-9 were significantly higher in AM-1 and AM-3 than MOE-1. Wnt-5a and Wnt signaling receptors, LRP and Frizzled, of both AM-1 and AM-3 were higher than MOE-1 in the mRNA level. Furthermore, expression and activity of MMP-9 in AM-3 increased dose- and time-dependently when stimulated with Wnt-3a protein. These results suggested that local invasiveness of ameloblastoma may be regulated by MMPs expression through the Wnt signaling pathway.
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Establishment of novel ameloblastoma-derived cell line, and the role of Wnt signaling pathway in ameloblastoma.2013
Author(s)
Toshiro Kibe, Takao Fuchigami, Michiko Kishida, Mikio Iijima, Hiroshi Hijioka, Akihiko Miyawaki, Ichiro Senba, tooru Kiyono, Norifumi Nakamura, Shosei Kisida
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Journal Title
Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology & Endodontics
Volume: 115
Related Report
Peer Reviewed
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[Journal Article] Immortalization and characterization of normal oral epithelial cells without using HPV and SV40 genes2011
Author(s)
Toshiro Kibe, Michiko Kishida, Masayuki Kamino, Mikio Iiima, Lin Clen, Mika Habu, Akihiko Miyawaki, Hiroshi Hijioka, Norifumi Nakamura, Tohru Kiyono, Shosei Kishida
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Journal Title
Oral Science International
Volume: 8(1)
Issue: 1
Pages: 20-28
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Immortalization and characterization of MOE1 cells, normal oral epithelial cell lines without using HPV and SV40 genes-.2011
Author(s)
Toshiro Kibe, Michiko Kidhida, Masayuki Kaimo,Mikiko Iiima, Lin Clen. Mika Habu, Akihiko, Miyawaki, Hiroshi Hijioka, Norifumi Nakamura, Tohru Kiyono, Shosei Kishida
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Journal Title
Oral Science International
Volume: 2
Pages: 20-28
Related Report
Peer Reviewed
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