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Possibility of bone substitute by using tissue engineering, concerning the bone grafting operation to the alveolar cleft of CL/CP.

Research Project

Project/Area Number 23592942
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Surgical dentistry
Research InstitutionKitasato University

Principal Investigator

YAMAZAKI Yasuharu  北里大学, 医学部, 講師 (00210401)

Co-Investigator(Kenkyū-buntansha) IKEMOTO Shigehiro  北里大学, 医学部, 助教 (90296492)
BABA Kyoko  北里大学, 医学部, 特別研修生 (90327411)
AOYAGI Kazuya  北里大学, 医学部, 講師 (10337959)
ISHIGURO Masashi  北里大学メディカルセンター, 医学部, 研究員 (40265640)
KUMAZAWA Kenichi  北里大学, 医学部, 助教 (60383618)
SUGIMOTO Takayuki  北里大学, 医学部, 助教 (20365133)
Co-Investigator(Renkei-kenkyūsha) OIDA Shinichiro  鶴見大学, 歯学部, 教授 (10114745)
MOCHIZIKI Junko  北里大学, 医学部, 講師 (90306613)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords未分化間葉系細胞 / 凍結保存 / 再生医工学
Research Abstract

Bone formation of Mesenchymal stromal cells(MSCs) derived from a surplus of maxillary bone fragments, in primary cleft lip / palate operation, was confirmed in vivo. When cultured with serum-free medium, maxilla-derived MSCs exhibited osteogenic potential in both in vitro and in vivo. With bone tissue-derived MSCs cryopreserved more than 10 years, in the safety assessment, no abnormality was found in G-band patterns as a morphologic investigation and in myc or p53 analysis. Moreover osteogenic potential and multipotency were maintained. Cryopreserved bone tissue-derived MSCs may be clinically useful.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (37 results)

All 2014 2013 2012 2011

All Journal Article (14 results) (of which Peer Reviewed: 14 results) Presentation (21 results) Book (2 results)

  • [Journal Article] Osteogenic Potential, Multipotency and Cytogenetic safe -ty of human bone tissue derived mesenchymal stromal cells (hBT -MSCs) after long-term cryo- preservation2014

    • Author(s)
      Kenichi Kumazawa, Takayuki Sugimoto, Yasuharu Yamazaki, Akira Takeda, Eiju Uchinuma
    • Journal Title

      The Kitasato Medical Journal

      Volume: 44 Pages: 95-103

    • URL

      http://mlib.kitasato-u.ac.jp/homepage/ktms/kaishi/pdf/KMJ44-1/KMJ44-1.html

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Assessment of the Osteogenic Potential of Maxilla- Derived Mesenchymal Stromal Cells and the Utilization of Serum-Free Medium for Culture Thereof2014

    • Author(s)
      Masashi Ishiguro, Yasuharu Yamazaki, Kyoko Baba,Kenichi Kumazawa, Takayuki Sugimoto, Akira Takeda, Eiju Uchinuma
    • Journal Title

      The Kitasato Medical Journal

      Volume: 44 Pages: 84-94

    • NAID

      40020030211

    • URL

      http://mlib.kitasato-u.ac.jp/homepage/ktms/kaishi/pdf/KMJ44-1/KMJ44-1.html

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Assessment of the Osteogenic Potential of Maxilla-Derived Mesenchymal Stromal Cells and the Utilization of Serum-Free Medium for Culture Thereof.2014

    • Author(s)
      Ishiguro M ,Yamazaki Y, Baba K、Kumazawa K, Sugimoto T, Takeda A, Uchinuma E
    • Journal Title

      The Kitasato Medical Journal

      Volume: 44 Pages: 84-94

    • NAID

      40020030211

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Osteogenic Potential, Multipotency, and Cytogenetic safety of human bone tissue-derived mesenchymal stromal cells (hBT-MSCs) after long-term cryopreservation.2014

    • Author(s)
      Kumazawa K, Sugimoto T, Yamazaki Y, Takeda A, Uchinuma E
    • Journal Title

      The Kitasato Medical Journal

      Volume: 44 Pages: 95-103

    • NAID

      40020030217

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Significance of Performing Osteo- genic Differentiation in Human Bone Tissue-Derived Mesenchymal Stromal Cells2013

    • Author(s)
      Takayuki Sugimoto, Yasuharu Yamazaki, Kenichi Kumazawa, Umiko Sone, Akira Takeda, Eiju Uchinuma
    • Journal Title

      Journal of oral tissue engi- neering

      Volume: 11 Pages: 103-112

    • URL

      http://www.jarde.jp/zasshi/e/abst11-2.html

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Osteogenic potential of human umbilical cord-derived mesenchymal stromal cells2013

    • Author(s)
      Kyoko Baba, Yasuharu Yamazaki, Akira Takeda, Eiju Uchinuma
    • Journal Title

      Journal of Cranio-Maxillo- facial Surgery

      Volume: 41 Issue: 8 Pages: 775-782

    • DOI

      10.1016/j.jcms.2013.01.025

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Significance of Performing Osteogenic Differentiation in Human Bone Tissue-Derived Mesenchymal Stromal Cells.2013

    • Author(s)
      Sugimoto T, Yamazaki Y, Kumazawa K, Sone U, Takeda A, Uchinuma E
    • Journal Title

      Journal of oral tissue engineering

      Volume: 11 Pages: 103-112

    • NAID

      130004941102

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Osteogenic potential of human umbilical cord-derived mesenchymal stromal cells.2013

    • Author(s)
      Baba K, Yamazaki Y, Akira Takeda, Uchinuma E
    • Journal Title

      ournal of Cranio-Maxillofacial Surgery

      Volume: in press

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Osteogenic Potential of Human Bone Marrow-Derived Mesenchymal Stromal Cells Cultured in Autologous Serum2012

    • Author(s)
      Akira Takeda, Yasuharu Yamazaki, Kyoko Baba, Masashi Ishiguro, Kazuya Aoyagi, Shigehiro Ikemoto, Eiju Uchinuma
    • Journal Title

      A preliminary Study Journal of Oral and Maxillofacial Surgery

      Volume: 70 Issue: 8 Pages: e469-e476

    • DOI

      10.1016/j.joms.2012.03.021

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Osteogenic potential of human um- bilical cord-derived mesenchymal stromal cells cultured with um- bilical cord blood-derived autoserum2012

    • Author(s)
      Kyoko Baba , Yasuharu Yamazaki, Shigehiro Ikemoto , Kazuya Aoyagi, Akira Takeda, Eiju Uchinuma
    • Journal Title

      Journal of Cranio- Maxillofacial Surgery

      Volume: 40 Issue: 8 Pages: 768-772

    • DOI

      10.1016/j.jcms.2012.02.006

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Osteogenic potential of human umbilical cord-derived mesenchymal stromal cells.2012

    • Author(s)
      Baba K, Yamazaki Y, Ikemoto S, Aoyagi K, Akira Takeda, Uchinuma E
    • Journal Title

      Journal of Cranio-Maxillofacial Surgery

      Volume: 40 Pages: 768-772

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Osteogenic Potential of Human Bone Marrow-Derived Mesenchymal Stromal Cells Cultured in Autologous Serum: A preliminary Study2012

    • Author(s)
      Takeda A, Yamazaki Y, Baba K, Ishiguro M, Aoyagi K, Ikemoto S, Uchinuma E
    • Journal Title

      Journal of Oral and Maxillofacial Surgery

      Volume: 70

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Osteogenic potential of human umbilical cord-derived mesenchymal stromal cells cultured with umbilical cord blood-derived autoserum2012

    • Author(s)
      Kyoko Baba, Yasuharu Yamazaki,et al
    • Journal Title

      Journal of Cranio-Maxillo-Facial Surgery

      Volume: In Press

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Osteogenic Potential of Human Bone Marrow-Derived Mesenchymal Stromal Cells Cultured in Autologous Serum: A preliminary Study2012

    • Author(s)
      Akira Takeda,Yasuharu Yamazaki, et al
    • Journal Title

      Journal of Oral and Maxillofacial Surgery

      Volume: In Press

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 長期凍結保存された骨組織由来間葉系細胞の骨形成能は代替骨として臨床応用可能である2014

    • Author(s)
      杉本孝之,熊澤憲一,山崎安晴,曽根由美子,武田啓,内沼栄樹
    • Organizer
      第13回日本再生医療学会総会
    • Place of Presentation
      国立京都国際会館京都
    • Related Report
      2013 Final Research Report
  • [Presentation] 長期凍結保存された骨組織由来間葉系細胞の骨形成能は代替骨として臨床応用可能である.2014

    • Author(s)
      杉本孝之, 山崎安晴 他
    • Organizer
      第13回日本再生医療学会総会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Annual Research Report
  • [Presentation] 凍結保存後臍帯血由来多血小板血漿中の成長因子含有量2013

    • Author(s)
      馬場香子,山崎安晴,熊澤憲一,杉本孝之,曽根由美子,武田啓,内沼栄樹
    • Organizer
      第22回日本形成外科学会基礎学術集会
    • Place of Presentation
      朱鷺メッセ新潟
    • Related Report
      2013 Final Research Report
  • [Presentation] 長期凍結保存された骨組織由来間葉系細胞の特性2013

    • Author(s)
      杉本孝之,熊澤憲一,山崎安晴,曽根由美子,武田啓,内沼栄樹
    • Organizer
      第22回日本形成外科学会基礎学術集会
    • Place of Presentation
      朱鷺メッセ新潟
    • Related Report
      2013 Final Research Report
  • [Presentation] 長期凍結保存された骨組織由来未分化間葉系細胞による代替骨組織の可能性2013

    • Author(s)
      熊澤憲一,山崎安晴,馬場香子,青栁和也,武田啓,曽根由美子,内沼栄樹
    • Organizer
      第12回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜神奈川
    • Related Report
      2013 Final Research Report
  • [Presentation] 凍結保存後の臍帯血由来多血小板血漿中PDGF-ββ・TGF-β・VEGF含有量2013

    • Author(s)
      馬場香子,山崎安晴,石黒匡史,熊澤憲一,曽根由美子,武田啓,内沼栄樹
    • Organizer
      第12回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜神奈川
    • Related Report
      2013 Final Research Report
  • [Presentation] 長期凍結保存後臍帯血由来多血小板血漿の成長因子含有量2013

    • Author(s)
      馬場香子,山崎安晴 他
    • Organizer
      第22回日本形成外科学会基礎学術集会
    • Place of Presentation
      朱鷺メッセ(新潟)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 長期凍結保存された骨組織由来間葉系細胞の特性2013

    • Author(s)
      杉本孝之, 山崎安晴 他
    • Organizer
      第22回日本形成外科学会基礎学術集会
    • Place of Presentation
      朱鷺メッセ(新潟)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 凍結保存後の臍帯血由来多血小板血漿中PDGF-ββ・TGF-β・VEGF含有量2013

    • Author(s)
      馬場香子、山崎安晴、石黒匡史、熊澤憲一、曽根由美子、武田啓、内沼栄樹
    • Organizer
      第12回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜 神奈川
    • Related Report
      2012 Research-status Report
  • [Presentation] 長期凍結保存された骨組織由来未分化間葉系 細胞による代替骨組織の可能性2013

    • Author(s)
      熊澤 憲一、山崎安晴、馬場香子、青栁和也、武田啓、曽根由美子、内沼栄樹
    • Organizer
      第12回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜 神奈川
    • Related Report
      2012 Research-status Report
  • [Presentation] 凍結保存後臍帯由来間葉系細胞のviability2012

    • Author(s)
      馬場香子,石黒匡史,山崎安晴,馬場香子,松尾あおい,武田啓,内沼栄樹
    • Organizer
      第21回日本形成外科学会基礎学術集会
    • Place of Presentation
      リステル猪苗代福島
    • Related Report
      2013 Final Research Report
  • [Presentation] 無血清培地で培養した上顎骨由来間葉系細胞の骨形成能‐FBS添加培地との比較検討2012

    • Author(s)
      石黒匡史,山崎安晴,馬場香子,池本繁弘,青柳和也,曽根由美子,武田啓,内沼栄樹
    • Organizer
      第21回日本形成外科学会基礎学術集会
    • Place of Presentation
      リステル猪苗代福島
    • Related Report
      2013 Final Research Report
  • [Presentation] 上顎骨由来間葉系幹細胞の骨形成能について2012

    • Author(s)
      熊澤憲一,山崎安晴,石黒匡史,馬場香子,池本繁弘,青栁和也,武田啓,曽根由美子,内沼栄樹
    • Organizer
      第21回日本形成外科学会基礎学術集会
    • Place of Presentation
      リステル猪苗代福島
    • Related Report
      2013 Final Research Report
  • [Presentation] 口唇裂・口蓋裂患者と凍結保存自己骨組織由来間葉系細胞の意義2012

    • Author(s)
      山崎安晴,池本繁弘,石渡靖夫,武田啓,内沼栄樹
    • Organizer
      第36回日本口蓋裂学会総会・学術集会
    • Place of Presentation
      国立京都国際会館京都
    • Related Report
      2013 Final Research Report
  • [Presentation] 長期凍結保存された骨組織由来の未分化間葉系細胞による代替骨組織の可能性2012

    • Author(s)
      熊澤憲一,山崎安晴,馬場香子,青柳和也,武田啓,曽根由美子,内沼栄樹
    • Organizer
      第11回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜神奈川
    • Related Report
      2013 Final Research Report
  • [Presentation] 口唇裂・口蓋裂患者と凍結保存自己骨組織由来間葉系細胞の意義.2012

    • Author(s)
      山崎安晴、池本繁弘、石渡靖夫、武田 啓、内沼栄樹
    • Organizer
      第36回日本口蓋裂学会総会・学術集会
    • Place of Presentation
      国立京都国際会館 京都
    • Related Report
      2012 Research-status Report
  • [Presentation] 上顎骨由来間葉系幹細胞の骨形成能について2012

    • Author(s)
      熊澤 憲、山崎安晴、石黒匡史、馬場香子、池本繁弘、青栁和也、 武田啓、曽根由美子、内沼栄樹
    • Organizer
      第21回日本形成外科学会基礎学術集会
    • Place of Presentation
      ホテルリステル猪苗代 福島
    • Related Report
      2012 Research-status Report
  • [Presentation] 無血清培地で培養した上顎骨由来間葉系細胞の骨形成能  ‐FBS添加培地との比較検討-2012

    • Author(s)
      石黒匡史、山崎安晴、馬場香子、、池本繁弘、 青柳和也、曽根由美子、武田啓、内沼栄樹
    • Organizer
      第21回日本形成外科学会基礎学術集会
    • Place of Presentation
      ホテルリステル猪苗代 福島
    • Related Report
      2012 Research-status Report
  • [Presentation] 凍結保存後臍帯由来間葉系細胞のviability2012

    • Author(s)
      馬場香子、石黒匡史、山崎安晴、松尾あおい、武田啓、内沼栄樹
    • Organizer
      第21回日本形成外科学会基礎学術集会
    • Place of Presentation
      ホテルリステル猪苗代 福島
    • Related Report
      2012 Research-status Report
  • [Presentation] 骨由来間葉系細胞のFBS添加培地,ヒト血清添加培地,無血清培地での骨形成比較検討2011

    • Author(s)
      石黒匡史,馬場香子,山崎安晴,武田啓,曽根由美子,内沼栄樹
    • Organizer
      骨由来間葉系細胞のFBS添加培地,ヒト血清添加培地,無血清培地での骨形成比較検討
    • Place of Presentation
      ハイアットリージェンシー東京 東京
    • Related Report
      2013 Final Research Report
  • [Presentation] 骨髄由来間葉系細胞のFBS添加培地、ヒト血清添加培地、無血清培地、での骨形成能比較検討2011

    • Author(s)
      石黒匡史,馬場香子,山崎安晴,他
    • Organizer
      第20回日本形成外科学会基礎学術集会
    • Place of Presentation
      ハイアットリ-ジェンシー東京
    • Related Report
      2011 Research-status Report
  • [Book] Cord Blood :Banks and Banking, Ethical Issues and Risks/Benefits2013

    • Author(s)
      Kyoko Baba, Yasuharu Yamazaki, Akira Takeda, Eiju Uchinuma
    • Publisher
      Nova Science Pub- lishers
    • URL

      https://www.novapublishers.com/catalog/product_info.php?products_id=37127

    • Related Report
      2013 Final Research Report
  • [Book] Cord Blood: Banks and Banking, Ethical Issues and Risks/Benefits.2013

    • Author(s)
      Kyoko Baba, Yasuharu Yamazaki, Akira Takeda, Eiju Uchinuma
    • Total Pages
      164
    • Publisher
      Nova Science Publishers
    • Related Report
      2013 Annual Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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