Detection of carcinoma cells in peripheral circulation by oncolgtic virus therapy

• Project/Area Number: 23592944
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Showa University
• Principal Investigator: KAMATANI Takaaki 昭和大学, 歯学部, 講師 (00315003)
• Co-Investigator(Kenkyū-buntansha): KONDO Seiji 昭和大学, 歯学部, 准教授 (10432634) ; MUKUDAI Yoshiki 昭和大学, 歯学部, 助教 (50325099) ; KURIHARA Yuuji 昭和大学, 歯学部, 助教 (90514969) ; 新谷 悟 昭和大学, 歯学部, 教授 (80294429)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 口腔癌 / 扁平上皮癌 / ウイルス増殖 / 口腔扁平上皮癌 / テロメライシン / E1遺伝子

Research Abstract

In this study, we investigated the detection of peripheral circulating tumor cells by an adenovirus included the GFP gene and the hTERT gene infected to oral carcinoma cells in circulating tumor cell model. Although we could confirm fluorescence in oral carcinoma cells, there were no relation between fluorescence and mRNA expression or protein expression. These results indicated that oral carcinoma cells would be circulate in peripheral blood flow in a certain period.