| Project/Area Number |
23592945
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TSUZUKI Masako 東京医科大学, 医学部, 助教 (40385092)
ABUKAWA Harutsugi 東京医科大学, 医学部, 助教 (50453717)
WATANABE Masato 東京医科大学, 医学部, 講師 (40349460)
HASEGAWA On 東京医科大学, 医学部, 助教 (50424619)
MIZUGUCHI Junichirou 東京医科大学, 医学部, 教授 (20150188)
CHIKAZU Daichi 東京医科大学, 医学部, 教授 (30343122)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 口腔癌 / 顎骨浸潤 / PTH / COX-2 |
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| Research Abstract |
The aims of this study were to investigate the role of parathyroid hormone (PTH) up regulation by the cyclooxygenase-2 (COX-2) signaling pathway in bone invasion using oral squamous cell carcinoma (OSCC) mouse bone invasion model and to clarify new treatments of OSCC invasion into the jaw bone. We used 2 mouse SCC lines (NR-S1, SCC7) with different expressions of PTHrP and COX-2. C3H/HeN mice were inoculated with these cells in the masseter region to establish an animal model of mandibular invasion by OSCC. We measured tumor sizes of the mice, analyzed mandibular invasion by OSCC using micro-CT, then examined the TRAP staining and number of osteoclasts, and determined the mRNA expression of osteoclast-related cytokines by real-time PCR. We suggest that osteoclast-related cytokines play important roles in the bone invasion by OSCC cells.
Consequently, PTHrP inhibition by COX-2 signaling is expected to be a therapeutic target to prevent bone invasion by OSCC cells.
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