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A new induction method for the controlled differentiation of human iPS cells using frozen sections

Research Project

Project/Area Number 23592950
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Surgical dentistry
Research InstitutionTsurumi University

Principal Investigator

TERADA Chika  鶴見大学, 歯学部, 学部助手 (40460216)

Co-Investigator(Kenkyū-buntansha) SATOMURA Kazuhito  鶴見大学, 歯学部, 教授 (80243715)
SATO Toru  鶴見大学, 歯学部, 講師 (30170765)
TOKUYAMA Reiko  鶴見大学, 歯学部, 助教 (20380090)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords再生医療 / iPS細胞 / 口腔粘膜由来iPS細胞 / 分化誘導 / 質の保証
Research Abstract

iPSCs themselves have some problems that need to be overcome. Briefly, a simple and efficient method has been expected for the induction the differentiation and the evaluation of quality of iPSCs. In this study, we hypothesized that it is possible to induce the controlled differentiation of iPSCs by culturing on frozen sections of every tissue/organ which have the most suitable microenvironment for the induction. As a result, iPSCs which were cultured on liver expressed mRNA and protein for hepatocyte related markers, and iPSCs which were cultured on brain/spinal cord expressed mRNA and protein for neuron related markers, respectively. Moreover, the differentiation efficiency of iPSCs on frozen sections could be different among clones of iPSCs. Judging from these facts, the induction method of human iPSCs using frozen sections could be useful as a simple and effective measure for inducing the differentiation of iPSCs and evaluating the potential and/or quality of iPSCs.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (10 results)

All 2014 2013 Other

All Presentation (10 results)

  • [Presentation] A new induction method for the controlled differentiation of human iPS cells using frozen sections2014

    • Author(s)
      Susumu Tadokoro
    • Organizer
      International Society for Stem Cell Research 12th Annual Meeting
    • Place of Presentation
      Vancouver
    • Related Report
      2013 Final Research Report
  • [Presentation] 凍結切片を用いたiPS細胞の新たな分化誘導法および品質評価法の確立2014

    • Author(s)
      田所晋
    • Organizer
      第14回日本抗加齢学会総会
    • Place of Presentation
      大阪国際会議場
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 凍結切片を用いたiPS細胞の新たな分化誘導法および品質評価法の確立2014

    • Author(s)
      田所晋
    • Organizer
      第13回日本再生医療学会総会
    • Place of Presentation
      国立京都国際会
    • Related Report
      2013 Final Research Report
  • [Presentation] 凍結切片を用いたiPS細胞の新たな分化誘導法および品質評価法の確立2014

    • Author(s)
      田所 晋
    • Organizer
      第13回日本再生医療学会総会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Annual Research Report
  • [Presentation] A new induction method for the controlled differentiation of human iPS cells using frozen sections.2014

    • Author(s)
      Susumu Tadokoro
    • Organizer
      International Society for Stem Cell Research 12th Annual Meeting
    • Place of Presentation
      バンクーバー
    • Related Report
      2013 Annual Research Report
  • [Presentation] 凍結切片を用いたiPS細胞の新たな分化誘導法の確立とそのiPS細胞品質評価への応用2014

    • Author(s)
      田所 晋
    • Organizer
      第59回日本口腔外科学会総会・学術大会
    • Place of Presentation
      幕張メッセ
    • Related Report
      2013 Annual Research Report
  • [Presentation] 凍結切片を用いたiPS細胞の新たな分化誘導法および品質評価法の確立2013

    • Author(s)
      田所晋
    • Organizer
      第78回鶴見大学歯学会例会
    • Place of Presentation
      鶴見大学会館メインホール
    • Year and Date
      2013-12-21
    • Related Report
      2013 Final Research Report
  • [Presentation] A new method for differentiation induction and quality evaluation of iPS cells using frozen sections2013

    • Author(s)
      田所晋
    • Organizer
      第58回日本口腔外科学会総会・学術大会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2013 Final Research Report
  • [Presentation] A new method for differentiation induction and quality evaluation of iPS cells using frozen sections.2013

    • Author(s)
      田所 晋
    • Organizer
      第58回日本口腔外科学会総会・学術大会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] 凍結切片を用いたiPS細胞の新たな分化誘導法および品質評価法の確立

    • Author(s)
      田所 晋
    • Organizer
      第78回鶴見大学歯学会例会
    • Place of Presentation
      鶴見大学会館メインホール
    • Related Report
      2013 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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