| Project/Area Number |
23592958
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
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| Research Collaborator |
RAZ Avraham Wayne State University, Karmanos Cancer Institute, Michigan, USA., 教授
WAKE Sou 東京医科歯科大学, 歯学部, 大学院生
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 遊走 / 上皮 / 間葉 / 浸潤・転移 / 発現 / 癌 |
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| Outline of Final Research Achievements |
Over-expression of AMF monomer induces motility of tumor cells and fibroblasts, at the same time forms homodimers in part. Molecular weight of AMF is 55 kD and 110 kD under non-reduced condition, and 65 kD under reduced condition. A point mutation of cDNA in the N terminal causes a replacement of amino acid, which results in loss of formation of homodimer. Over-expression of point-mutated AMF induced cell motility via high affinity receptors, however, waives ability to induce epithelial-to-mesenchymal transition. Thus, formation of homodimers of AMF is strongly suggested to be critical to induce epithelial-to-mesenchyal transion by way of low affinity receptors.
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