Expression and functional analysis of RHAMM in human salivary gland carcinomas

• Project/Area Number: 23592963
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Hiroshima University
• Principal Investigator: SHIGEISHI HIDEO 広島大学, 医歯薬保健学研究院, 助教 (90397943)
• Co-Investigator(Kenkyū-buntansha): KAMATA Nobuyuki 広島大学, 医歯薬保健学研究院, 教授 (70242211) ; HIGASHIKAWA Koichiro 広島大学, 病院, 講師 (80363084) ; ONO Shigehiro 広島大学, 医歯薬保健学研究院, 助教 (70379882)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: RHAMM / 唾液腺癌 / 口腔扁平上皮癌 / 癌幹細胞 / 口腔癌

Research Abstract

In head and neck cancers, elevated expression of RHAMM was associated with high proliferation of cancer cells. In addition, we demonstrated that there were two biologically different cancer stem cell (CSC) phenotypes of salivary gland carcinoma and oral squamous cell carcinoma, one of which is CD44high/ epithelial specific antigen (ESA) low cells, which have mesenchymal characteristics and are migratory, while the other phenotype is CD44high/ESAhigh cells, which have epithelial properties and high proliferative capacity, and harbor increased RHAMM expression. Since RHAMM is required for holoclone forming of CD44high/ESAhigh cells, it appears that it has a role in the self-renewal ability of head and neck CSC.

Research Products

• [Journal Article] Role of receptor for hyaluronan-mediated motility (RHAMM) in human head and neck cancers (2014)
  • Author(s): Shigeishi H, Higashikawa K, Takechi M
  • Journal Title: J Cancer Res Clin Oncol Volume: (印刷中) Issue: 10 Pages: 1629-1640
  • DOI: 10.1007/s00432-014-1653-z
  • Peer Reviewed
• [Journal Article] 口腔扁平上皮癌における癌幹細胞の特性(総説) (2013)
  • Author(s): 重石英生、東川晃一郎、奥井岳、小野重弘、太田耕司、武知正晃、杉山勝、鎌田伸之
  • Journal Title: 日本口腔組織培養学会誌 Volume: 22(2) Pages: 1-6
  • Peer Reviewed
• [Journal Article] Autocrine galectin-1 promotes collective cell migration of squamous cell carcinoma cells through up-regulation of distinct integrins. (2013)
  • Author(s): Rizqiawan A, Tobiume K, Okui G, Yamamoto K, Shigeishi H, Ono S, Shimasue H, Takechi M, Higashikawa K, Kamata N.
  • Journal Title: .Biochem Biophys Res Commun. Volume: 441(4) Issue: 4 Pages: 904-910
  • DOI: 10.1016/j.bbrc.2013.10.152
  • Peer Reviewed
• [Journal Article] Maintenance of stem cell self-renewal in head and neck cancers requires actions of GSK3β influenced by CD44 and RHAMM. (2013)
  • Author(s): Shigeishi H, Biddle A, Gammon L, Emich H, Rodini CO, Gemenetzidis E, Fazil B, Sugiyama M, Kamata N, Mackenzie IC.
  • Journal Title: Stem Cells Volume: 31 Issue: 10 Pages: 2073-83
  • DOI: 10.1002/stem.1418
  • Peer Reviewed
• [Journal Article] AKT primes Snail-induced EMT concomitantly with the collective migration of squamous cells. (2013)
  • Author(s): Okui G, Tobiume K, Rizqiawan A, Yamamoto K, Shigeishi H, Ono S, Higashikawa K, Kamata N
  • Journal Title: J Cell Biochem Volume: (In press) Issue: 9 Pages: 2039-2049
  • DOI: 10.1002/jcb.24545
  • Peer Reviewed
• [Journal Article] Snail promotes Cyr61 secretion to prime collective cell migration and form invasive tumor nests in squamous cell carcinoma. (2013)
  • Author(s): Tanaka F, Rizqiawan A, Higashikawa K, Tobiume K, Okui G, Shigeishi H, Ono S, Shimasue H, Kamata N.
  • Journal Title: Cancer Lett Volume: 329(2) Issue: 2 Pages: 243-252
  • DOI: 10.1016/j.canlet.2012.11.023
  • Peer Reviewed
• [Journal Article] 口腔扁平上皮癌における癌幹細胞の特性（総説） (2013)
  • Author(s): 重石 英生、東川 晃一郎、奥井 岳、小野 重弘、太田 耕司、武知 正晃、杉山 勝、鎌田 伸之
  • Journal Title: 日本口腔組織培養学会誌 Volume: 22 Pages: 1-6
  • Peer Reviewed
• [Journal Article] Autocrine galectin-1 promotes collective cell migration of squamous cell carcinoma cells through up-regulation of distinct integrins. (2013)
  • Author(s): Rizqiawan A, Tobiume K, Okui G, Yamamoto K, Shigeishi H, Ono S, Shimasue H, Takechi M, Higashikawa K, Kamata N.
  • Journal Title: Biochem Biophys Res Commun Volume: 441 Pages: 904-910
  • Peer Reviewed
• [Journal Article] Expression of receptor for hyaluronan-mediated motility (RHAMM) in ossifying fibromas. (2013)
  • Author(s): Hatano H, Ogawa I, Shigeishi H, Kudo Y, Ohta K, Higashikawa K, Takechi M, Takata T, Kamata N.
  • Journal Title: Histol Histopathol Volume: 28 Pages: 473-80
  • Peer Reviewed
• [Journal Article] Maintenance of stem cell self-renewal in head and neck cancers requires actions of GSK3β influenced by CD44 and RHAMM (2013)
  • Author(s): Hideo Shigeishi, Adrian Biddle, Luke Gammon, Helena Emich, Camila.O. Rodini, Emilios Gemenetzidis, Bilal Fazil, Masaru Sugiyama, Nobuyuki Kamata, Ian C. Mackenzie
  • Journal Title: STEM CELLS Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Increased telomerase activity and hTERT expression in human salivary gland carcinomas (2011)
  • Author(s): Shigeishi H, Sugiyama M, Tahara H, Ono S, Kumar Bhawal U, Okura M, Kogo M, Shinohara M, Shindoh M, Shintani S, Hamakawa H, Takata T, Kamata N
  • Journal Title: Oncol Lett Volume: 1;2(5) Pages: 845-885
  • DOI: 10.3892/ol.2011.354
  • Peer Reviewed
• [Journal Article] RHAMM / ERK interaction induces proliferative activities of cementifying fibroma cells through a mechanism based on the CD44-EGFR (2011)
  • Author(s): Hatano H, Shigeishi H, Kudo Y, Higashikawa Y, Tobiume K, Takata T, Kamata N
  • Journal Title: Lab Invest Volume: 91(3) Issue: 3 Pages: 379-391
  • DOI: 10.1038/labinvest.2010.176
  • Peer Reviewed
• [Journal Article] PGE_2 targets squamous cell carcinoma cell with the activated epidermal growth factor receptor family for survival against 5-fluorouracil through NR4A2 induction (2011)
  • Author(s): 重石英生
  • Journal Title: Cancer Letters Volume: 307 Issue: 2 Pages: 227-236
  • DOI: 10.1016/j.canlet.2011.04.008
  • Peer Reviewed
• [Journal Article] PGE2 targets squamous cell carcinoma cell with the activated epidermal growth factor receptor family for survival against 5-fluorouracil through NR4A2 induction. (2011)
  • Author(s): Shigeishi H, Higashikawa K, Hatano H, Okui G, Tanaka F, Tran TT, Rizqiawan A, Ono S, Tobiume K, Kamata N.
  • Journal Title: Cancer Letter Volume: 28 Pages: 227-36
  • Peer Reviewed
• [Journal Article] Increased telomerase activity and hTERT expression in human salivary gland carcinomas (2011)
  • Author(s): Shigeishi H, Sugiyama M, Tahara H, Ono S, Ujjal Kumar Bhawal, Okura M, Kogo M, Shinohara M, Shindoh M, Shintani S, Hamakawa H, Takata T, Kamata N.
  • Journal Title: Oncology Letters Volume: 2 Pages: 2011-2011
  • Peer Reviewed
• [Presentation] 口腔扁平上皮癌細胞における幹細胞形質とGSK3βの関わり (2013)
  • Author(s): 重石英生、奥井岳、小野重弘、Andra Rizqiawan、東川晃一郎、杉山勝,鎌田伸之
  • Organizer: 第67回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 栃木
  • Year and Date: 2013-05-24
• [Presentation] Role of galectin 1 in cell migration and invasiveness in human squamous cell carcinoma correlated with EMT (2013)
  • Author(s): ANDRA RIZQIAWAN,飛梅圭、奥井岳、山本一博、東川晃一郎、重石英生、小野重弘、鎌田伸之
  • Organizer: 第67回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 栃木
  • Year and Date: 2013-05-24
• [Presentation] 口腔扁平上皮癌幹細胞におけるCD44関連シグナル伝達経路の役割 (2012)
  • Author(s): 重石英生、奥井岳、小野重弘、東川晃一郎、杉山勝、鎌田伸之
  • Organizer: 第49回日本口腔組織培養学会学術大会
  • Place of Presentation: 広島
  • Year and Date: 2012-11-17
• [Presentation] CD44-related molecular events underline the choice between self-renewal and differentiation of cancer stem cells in SCC (2012)
  • Author(s): Hideo Shigeishi, Koichiro Higashikawa, Shigehiro Ono, Masaru Sugiyama, Nobuyuki Kamata
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
  • Year and Date: 2012-09-19
• [Presentation] Relevance of EMT in vitro models for OSCC invasion studies (2012)
  • Author(s): Rondini C, Fazil B, Emich H, Bidle A, Gammon L, Gmmenetzidis E, Shigeishi H, Mackenzie C
  • Organizer: IADR 90th General Session
  • Place of Presentation: Brazil
• [Presentation] 口腔扁平上皮癌細胞における幹細胞形質とGSK3βの関わり
  • Author(s): 重石 英生、奥井 岳、小野 重弘、Andra Rizqiawan、東川 晃一郎、杉山 勝, 鎌田 伸之
  • Organizer: 第67回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 栃木
• [Presentation] CD44-related molecular events underline the choice between self-renewal and differentiation of cancer stem cells in SCC
  • Author(s): 重石 英生、東川 晃一郎、小野 重弘、杉山 勝、鎌田 伸之
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] 口腔扁平上皮癌幹細胞におけるCD44関連シグナル伝達経路の役割
  • Author(s): 重石 英生、奥井 岳、小野 重弘、東川 晃一郎、杉山 勝、鎌田 伸之
  • Organizer: 第49回 日本口腔組織培養学会学術大会
  • Place of Presentation: 広島