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Clarification of proliferation and deveropment mechanism as the targetting cytokine in oral cancer

Research Project

Project/Area Number 23592974
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Surgical dentistry
Research InstitutionMeikai University

Principal Investigator

SAKASHITA Hideaki  明海大学, 歯学部, 教授 (10178551)

Co-Investigator(Kenkyū-buntansha) OHMORI Yoshihiro  明海大学, 歯学部, 教授 (50194311)
FUKUDA Masakatsu  明海大学, 歯学部, 講師 (10311614)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords口腔癌 / サイトカイン / p53 / IL-23 / TNF-α / アポトーシス / NF-κB
Research Abstract

IL-23 was constitutively expressed in oral cancer cells. The up-regulation of IL-23 expression was observed, especially in p53 mutant cells. In contrast, the reduced expression of IL-23 was observed in SAS cells of p53 wild type. p53 knockdown in SAS cells led to increase IL-23 mRNA expression. However, there was no significant difference in IL-23 protein level in the transfected-control siRNA cells. Then, when the p53 knockdowned-SAS cells were stimulated with TNF-alpha, a time-dependent increase in the IL-23 protein expression was observed. These data indicate a possibility that IL-23 expression is regulated by p53. In addition, the localization of p53 and IL-23 in the biopsy samples of oral cancer was examined using an immunohistochemical method. The positive reaction for IL-23 was observed in 10 of 22 (p53 positive) cases (45.5%) of SCC. These data suggest that p53 gene alteration leads the growth and proliferation of oral cancer by the enhanced IL-23 expression.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (20 results)

All 2013 2012 2011 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (12 results) Book (2 results)

  • [Journal Article] 口腔扁平上皮癌におけるp53依存性IL-23の発現2013

    • Author(s)
      菅原良和,福田正勝,坂下英明
    • Journal Title

      日本小児口腔外科学会雑誌

      Volume: Vol.22,No.2 Pages: 131-139

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 口腔扁平上皮癌におけるp53依存性IL-23の発現2013

    • Author(s)
      菅原良和,福田正勝,坂下英明
    • Journal Title

      日本小児口腔外科学会雑誌

      Volume: 22 Pages: 131-139

    • Related Report
      2013 Annual Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Molecular insights into the proliferation and progression mechanisms of the oral cancer: strategies for the effective and personalized therapy2012

    • Author(s)
      Masakatsu Fukuda, Kaoru Kusama, Hideaki Sakashita
    • Journal Title

      Japanese Dental Science Review

      Volume: 48 Pages: 23-41

    • NAID

      10030281789

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular insights into the proliferation and progression mechanisms of the oral cancer: strategies for the effective and personalized therapy.2012

    • Author(s)
      Fukuda M, Kusama K, Sakashita H
    • Journal Title

      Japanese Dental Science Review

      Volume: 48 Issue: 1 Pages: 23-41

    • DOI

      10.1016/j.jdsr.2011.08.001

    • NAID

      10030281789

    • Related Report
      2012 Research-status Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] 口腔扁平上皮癌におけるInterleukin (IL)-23の発現と動態について2011

    • Author(s)
      田中真,福田正勝,菅原良和,柳下治男,草間薫,坂下英明
    • Journal Title

      Hospital Dentistry & Oral-Maxillofacial Surgery

      Volume: 22 Pages: 163-173

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 口腔扁平上皮癌におけるInterleukin (IL)-23の発現と動態について.2011

    • Author(s)
      田中 真,福田正勝,菅原良和,柳下治男,草間 薫,坂下英明
    • Journal Title

      Hospital Dentistry & Oral-Maxillofacial Surgery

      Volume: 22 Pages: 163-173

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Resveratrol Induces Autophagic Cell Death In Or al Squamous Cell Carcinoma2013

    • Author(s)
      Masakatsu FUKUDA, Syota TAKIZAWA, Yukihiro KAWAMOTO, Yoshito OHYAMA and Hideaki SAKASHITA
    • Organizer
      2^<nd> Meeting of the International Association for Dental Research (IADR)–Asia Pacific Region to be held at the Plaza Athenee
    • Place of Presentation
      Bangkok, Thailand
    • Related Report
      2013 Final Research Report
  • [Presentation] Resveratrol Induces Autophagic Cell Death In Oral Squamous Cell Carcinoma2013

    • Author(s)
      Masakatsu FUKUDA, Syota TAKIZAWA, Yukihiro KAWAMOTO, Yoshito OHYAMA and Hideaki SAKASHITA
    • Organizer
      2nd Meeting of the International Association for Dental Research (IADR)
    • Place of Presentation
      Bangkok, Thailand
    • Related Report
      2013 Annual Research Report
  • [Presentation] レスベラトロールは口腔扁平上皮癌に自己貪食型細胞死を誘導する2013

    • Author(s)
      福田正勝,瀧澤将太,大山嘉人,川本幸寛,鈴木正二,坂下英明
    • Organizer
      第58回日本口腔外科学会学術大会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] p53 gene regulates inter leukin (IL)-23 to prevent growth and prolifer ating activity of oral squamous cell carcinomas2012

    • Author(s)
      Masakatsu Fukuda, Kaoru Kusama and Hideaki Sakashita
    • Organizer
      UICC World Cancer Congress
    • Place of Presentation
      Montréal, Canada
    • Related Report
      2013 Final Research Report
  • [Presentation] p53 gene regulates interleukin (IL)-23 to prevent growth and proliferating activity of oral squamous cell carcinomas.2012

    • Author(s)
      Masakatsu Fukuda, Kaoru Kusama and Hideaki Sakashita
    • Organizer
      UICC World Cancer Congress
    • Place of Presentation
      Montreal, Canada
    • Related Report
      2012 Research-status Report
  • [Presentation] 口腔扁平上皮癌におけるアデノウィルス受容体(CAR; CXADR)の発現について2012

    • Author(s)
      福田正勝,井上勝元,奥 結香,瀧澤将太,大山嘉人,川本幸寬,鈴木正二,坂下英明
    • Organizer
      第57回日本口腔外科学会総会・学術大会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] MALT1による口腔癌細胞の変動タンパク質と増殖能について2012

    • Author(s)
      川本幸寬,大山嘉人,瀧澤将太,横塚裕二,篠原優紀,福田正勝,千葉忠成,坂下英明
    • Organizer
      第57回日本口腔外科学会総会・学術大会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] Interleukin (IL)-23 promotes growth and proliferating activity of oral squamous cell carcinomas2011

    • Author(s)
      Masakatsu Fukuda, Kaoru Kusama and Hideaki Sakashita
    • Organizer
      International Conference & Exhibition on Cancer Science & Therapy
    • Place of Presentation
      Las Vegas, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] 口腔扁平上皮癌におけるRCAS1の発現とその機能について2011

    • Author(s)
      福田正勝,大山嘉人,川本幸寛,瀧澤将太,鈴木正二,坂下英明
    • Organizer
      第56回日本口腔外科学会学術総会
    • Place of Presentation
      大阪国際会議場
    • Related Report
      2013 Final Research Report 2011 Research-status Report
  • [Presentation] Analysis of proliferation and invasion in oral squamous cell carcinoma which BRAK/CXCL14-targeted2011

    • Author(s)
      福田正勝,坂下英明
    • Organizer
      第14回明海歯科医学会
    • Place of Presentation
      明海大学歯学部
    • Related Report
      2011 Research-status Report
  • [Presentation] Analysis of proliferation and invasion in oral squamous cell carcinoma which p53/R2-targeted2011

    • Author(s)
      Masakatsu Fukuda, Kaoru Kusama and Hideaki Sakashita
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Related Report
      2011 Research-status Report
  • [Presentation] Analysis of pr olifer ation and invasion in or al squamous cell car cinoma which BRAK/CXCL14-tar geted

    • Author(s)
      福田正勝
    • Organizer
      第14回明海歯科医学会
    • Related Report
      2013 Final Research Report
  • [Book] The Role of Tumor Microenvironment in Oral Cancer2012

    • Author(s)
      Masakatsu Fukuda, Yoshihiro Ohmori and Hideaki Sakashita
    • Publisher
      IN Tumor Microenvironment and Myelomonocytic Cells
    • Related Report
      2013 Final Research Report
  • [Book] The Role of Tumor Microenvironment in Oral Cancer. IN Tumor Microenvironment and Myelomonocytic Cells, (S.K. Biswas, ed.)2012

    • Author(s)
      Masakatsu Fukuda, Yoshihiro Ohmori and Hideaki Sakashita
    • Publisher
      InTech, Rijeka, Croatia
    • Related Report
      2012 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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