| Project/Area Number |
23592975
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OKABE Sadao 日本歯科大学, 生命歯学部, 客員教授 (70573731)
SHIMAZU Yoshihito 日本歯科大学, 生命歯学部, 講師 (10297947)
AOBA Takaaki 日本歯科大学, 生命歯学部, 教授 (30028807)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 歯学 / 病理学 / 舌癌 / 浸潤 / 組織立体構築 / 病理診断 |
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| Research Abstract |
The present study aimed to develop a clinical-pathological interactive diagnosis system, where we intend to correlate macroscopic mucosal changes with intraepithelial phenotypic alterations. To this goal, the degree of intraepithelial malignancy is classified into 4 grades, depending on CK13 down-expression and Ki-67 localization. The immunohistology-based 3D reconstruction helped to elucidate intraepithelial alterations taking place with premalignant to malignant transition and to assess quantitatively the number and size of cell clusters possessing discrete differentiation/proliferation phenotypes. The 3D analyses also delineated architectural abnormalities associated with the dysplasia-invasion transition. The present immunophenotypic evaluation disclosed complicated distribution of epithelial phenotypes and the degree of proliferative capacity in oral epithelial lesions.
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