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A study of tooth root formation by a novel co-culture system -Three Dimensional and Layered (TDL) culture.

Research Project

Project/Area Number 23592991
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Surgical dentistry
Research InstitutionOkayama University

Principal Investigator

MATSUMURA TATSUSHI  岡山大学, 医歯(薬)学総合研究科, 助教 (70432648)

Co-Investigator(Kenkyū-buntansha) TABATA Jun  東京医科歯科大学, 大学院医歯(薬)学総合研究科, 准教授 (20243248)
HIRATA Azumi  大阪医科大学, 医学部, 助教 (40263587)
YAMACHIKA Eiki  岡山大学, 大学病院, 講師 (10294422)
MORITANI Norifumi  岡山大学, 大学院医歯(薬)学総合研究科, 助教 (60467751)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords歯根形成 / 再生医学
Research Abstract

Tooth root formation is a complex process and the mechanism remains largely unclear due to the difficulty to mimic the procedure. Thus it is extensively necessary to establish the experimental model of tooth root formation. In this study we tried to develop tooth root formation model using Three Dimensional and Layered (TDL) culture that we engineered. The isolation of Hertwig's Epithelial Root Sheath/Epithelial Rest of Malassez (HER/ERM) to culture is challenging, however, our data revealed that perlecan might be one of the cell markers of dental pulp cells. Interestingly, ERM was not observed in rat incisor. ERM is transformed during 'elongation' and 'fragmentation' of HERS. It is possible that discreteness may not occur in HERS or the discrete residual cells may disappear resulting in no evidence of ERM. To understand the variety of events including HERS/ERM during root formation, further investigation is needed and our TDL culture is essential to develop the experimental model.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (9 results)

All 2012 2011

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (6 results)

  • [Journal Article] Bone quality and quantity of the anterior maxillary trabecular bone in dental implants sites2012

    • Author(s)
      Mari Wakimoto, Tatsushi Matsumura, Takaaki Ueno, Nobuyoshi Mizukawa, Seiji Iida
    • Journal Title

      Clin.Oral Impl.Res.(E Pub)

      Volume: Vol.23、No.11 Issue: 11 Pages: 1314-1319

    • DOI

      10.1111/j.1600-0501.2011.02347.x

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Target genes of the largest human SWI/SNF complex subunit control cell growth2011

    • Author(s)
      Inoue H, Giannakopoulos S, Parkhurst CN, Matsumura T, Kono EA, Furukawa T, Tanese N
    • Journal Title

      Biochem J

      Volume: Vol.432、No.1 Issue: 1 Pages: 83-92

    • DOI

      10.1042/bj20101358

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Target genes of the largest human SWI/SNF complex subunit control cell growth.2011

    • Author(s)
      Inoue H, Giannakopoulos S, Parkhurst CN, Matsumura T, Kono EA, Furukawa T, Tanese N.
    • Journal Title

      Biochem J.

      Volume: 434(1) Pages: 83-92

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] OVXマウスにおけるDHNAの骨吸収抑制効果第二報骨量に対する統計学的解析2012

    • Author(s)
      喜多憲一郎、山近英樹、松原正和、石田展久、森谷徳文、松村達志、藤田佑貴、高畠清文、飯田征二
    • Organizer
      第57回日本口腔外科学会総会・学術大会
    • Place of Presentation
      横浜
    • Related Report
      2013 Final Research Report
  • [Presentation] マウス骨髄由来細胞および皮質骨由来細胞による骨再生2012

    • Author(s)
      山近英樹、喜多憲一郎、松原正和、高畠清文、仲田直樹、石田展久、水川展吉、松村達志、高木愼、飯田征二
    • Organizer
      第57回日本口腔外科学会総会・学術大会
    • Place of Presentation
      横浜
    • Related Report
      2013 Final Research Report
  • [Presentation] OVXマウスにおけるDHNAの骨吸収抑制効果 第二報 骨量に対する統計学的解析.2012

    • Author(s)
      喜多憲一郎, 山近英樹, 松原正和, 石田展久, 森谷徳文, 松村達志, 藤田佑貴, 高畠清文, 飯田征二
    • Organizer
      第57回日本口腔外科学会総会・学術大会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] マウス骨髄由来細胞および皮質骨由来細胞による骨再生.2012

    • Author(s)
      山近英樹, 喜多憲一郎, 松原正和, 高畠清文, 仲田直樹, 石田展久, 水川展吉, 松村達志, 高木 愼, 飯田征二
    • Organizer
      第57回日本口腔外科学会総会・学術大会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] Bone quality and quantity of the anterior maxillary trabecular bone in dental implant sites2011

    • Author(s)
      Wakimoto M, Matsumura T, Ueno T, Ota A, Mizukawa N, Yanagi Y, Iida S
    • Organizer
      20th Annual Scientific Meeting of the European Association of Osseointegration
    • Place of Presentation
      Athens, Greece
    • Related Report
      2013 Final Research Report
  • [Presentation] Bone quality and quantity of the anterior maxillary trabecular bone in dental implant sites.2011

    • Author(s)
      Wakimoto M, Matsumura T, Ueno T, Ota A, Mizukawa N, Yanagi Y, Iida S.
    • Organizer
      20th Annual Scientific Meeting of the European Association of Osseointegration.
    • Place of Presentation
      Athens, Greece
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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