The post-transcriptional regulatory system of gene expressionof in chondrocytes by ncleophosmin

• Project/Area Number: 23592998
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Showa University
• Principal Investigator: MUKUDAI Yoshiki 昭和大学, 歯学部, 助教 (50325099)
• Co-Investigator(Kenkyū-buntansha): SATORU Shintani 昭和大学, 歯学部, 教授 (80294429) ; SEIJI Kondo 昭和大学, 歯学部, 准教授 (10432634)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: 軟骨 / 骨 / ｍRNA / ヌクレオホスミン / 転写後制御 / RNA安定性 / B23 / RNA / 3'-UTR / 転写後遺伝子発現制御 / cis-element / 転写後発現制御 / cis-elemment

Research Abstract

We successfully cloned mRNA, which bound to NPM protein in ATDC5 cells (a mouse chondrosarcoma cell line). The gene was found to bind to NPM by REMSA assay, and furthermore, to regulate the growth and differentiation of chondrocytes invivo, utilizing a gene expression and knock-down techniques.

Research Products

• [Journal Article] Differential expression of vascular endothelial growth factor in high- and low-metastasis cell lines of salivary gland adenoid cystic carcinoma (2014)
  • Author(s): Kondo, S., Mukudai, Y., Soga, D., Nishida, T., Takigawa, M., Shirota, T
  • Journal Title: Anticancer Res Volume: 34(2) Pages: 671-7
• [Journal Article] Potential anti-osteoporotic effects of herbal extracts on osteoclasts, osteoblasts and chondrocytes in vitro (2014)
  • Author(s): Mukudai, Y, Kondo, S., Koyama, T., Li C., Banka, S., Kogure, A., Yazawa, K., Shintani, S
  • Journal Title: BMC Complement Altern Med Volume: 14 Pages: 29-29
• [Journal Article] Potential anti-osteoporotic effects of herbal extracts on osteoclasts, osteoblasts and chondrocytes in vitro. (2014)
  • Author(s): *Mukudai, Y, Kondo, S., Koyama, T., Li C., Banka, S., Kogure, A., Yazawa, K., Shintani, S.
  • Journal Title: BMC Complement Altern Med. Volume: 14 Issue: 1 Pages: 29-29
  • DOI: 10.1186/1472-6882-14-29
  • Peer Reviewed / Open Access
• [Journal Article] The root bark extracts of Juncus effusuc and Paeonia suffruticosa protect salivary gland acinar cells from apoptotic cell death induced by cis-platinum (II) diammine dichloride (2013)
  • Author(s): Koyama, T., Li, C., Yazawa, K., Shintani, S
  • Journal Title: Oncol Rep Volume: 30 Pages: 2665-71
• [Journal Article] Tumor protein D54 is a negative regulator of extracellular matrix-dependent migration and attachment in oral squamous cell carcinoma-derived cell lines (2013)
  • Author(s): Mukudai, Y., Kondo, S., Fujita, A., Yoshihama, Y., Shirota, T., Shintani, S
  • Journal Title: Cellular Oncol Volume: 36 Pages: 233-245
• [Journal Article] Tumor protein D54 is a negative regulator of extracellular matrix-dependent migration and attachment in oral squamous cell carcinoma-derived cell lines (2013)
  • Author(s): Mukudai Y*, Kondo S, Fujita A, Yoshihama Y, Shirota T, Shintani S.
  • Journal Title: Cell Oncol (Dordr). Volume: in press Issue: 3 Pages: 233-245
  • DOI: 10.1007/s13402-013-0131-y
  • Peer Reviewed
• [Journal Article] A combination of chemical and mechanical stimuli enhances not only osteo- but also chondro-differentiation in adipose-derived stem cells (2012)
  • Author(s): Banka
  • Journal Title: J Oral Biosciences Volume: 54 Issue: 4 Pages: 188-195
  • DOI: 10.1016/j.job.2012.10.002
  • Peer Reviewed
• [Presentation] 灯心草および牡丹皮エキスはシ正常唾液腺房細胞をシスプラチンによるアポトーシスから保護する (2013)
  • Author(s): 椋代義樹、近藤誠二、小山智之、Chunnan Li、矢澤一良、新谷悟
  • Organizer: 第55回歯科基礎医学会学術大会
  • Place of Presentation: 岡山
  • Year and Date: 2013-09-21
• [Presentation] 苦楝皮、醋延胡索および白薇抽出液の骨粗鬆症治療への基礎的研究 (2013)
  • Author(s): 椋代義樹、近藤誠二、新谷悟
  • Organizer: 第31回日本骨代謝学会
  • Place of Presentation: 神戸
  • Year and Date: 2013-05-30
• [Presentation] 新たな骨粗鬆症治療薬となりうる生薬エキスの検索 (2013)
  • Author(s): 椋代義樹、近藤誠二、小山智之、Chunnan Li、矢澤一良、新谷悟
  • Organizer: 昭和大学歯学部文部科学省私立大学戦略的研究基盤形成支援事業平成24年度シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2013-03-23
• [Presentation] 新規癌特異的遺伝子TPD54は口腔扁平上皮癌細胞の細胞外基質依存性遊走能を抑制する (2012)
  • Author(s): 椋代義樹、近藤誠二、藤田温志、新谷悟
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-14
• [Presentation] 新たな骨粗鬆症治療薬となりうる生薬エキスの探索 (2012)
  • Author(s): 椋代義樹、近藤誠二、小山智之、Li chunnan、矢澤一良、新谷悟
  • Organizer: 第49回日本口腔組織培養学会学術大会
  • Place of Presentation: 広島
  • Year and Date: 2012-11-17
• [Presentation] 新規癌特異的遺伝子TPD54は口腔扁平上皮癌細胞の細胞外基質依存性遊走能を抑制する (2012)
  • Author(s): 椋代義樹、近藤誠二、藤田温志、吉濱泰斗、代田達夫、新谷悟
  • Organizer: 第57回日本口腔外科学会総会・学術大会
  • Place of Presentation: 横浜
  • Year and Date: 2012-10-20