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Osteogenic and chondrogenic induction by NELL 1

Research Project

Project/Area Number 23592999
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Surgical dentistry
Research InstitutionShowa University

Principal Investigator

ODA Mitsuo  昭和大学, 歯学部, 兼任講師 (30359492)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Masanori  昭和大学, 歯学部, 教授 (50180394)
MAYAHARA Mitsuori  昭和大学, 歯学部, 助教 (30384184)
Co-Investigator(Renkei-kenkyūsha) KURODA Shunichi  名古屋大学, 農学部, 教授 (60263406)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsNELL / 骨誘導 / 軟骨誘導 / 骨形成 / NELL1
Research Abstract

NELL1 is a secretory protein of about 110 kDa having both six EGF-like sequence and five vWF-C-like sequence. NELL1 does not show the ectopic osteoinduction unlike BMP and has the bone inducibility equal to BMP in vitro. Application of 10 microgram of NELL1 in the rat skull defect model showed the rapid osteogenesis. However, the increase of the NELL1 dose did not enhance the more rapid onteoinduction. In the case of the rat tibia defect model, 2 microgram of NELL1 induced the active bone induction. In this study, we confirm the osteoinducibility of 2 microgram of NELL1 in both models. We also examine the effect of NELL1 at low (0.5 microgram) and high (10 microgram) dose and sbhow the possibility of the clinical application of NELL1.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (43 results)

All 2014 2013 2012 2011 Other

All Journal Article (17 results) (of which Peer Reviewed: 4 results) Presentation (24 results) Book (2 results)

  • [Journal Article] Composition of the blood supply in the sublingual and submandibular spaces and its relationship to the lateral lingual foramen of the mandible2014

    • Author(s)
      Nakajima K, Tagaya A, Otonari-Yamamoto M, Seki K, Araki K, Sano T, Okano T, Nakamura M
    • Journal Title

      Oral Surg Oral Med Oral Pathol Oral Radiol

      Volume: 117

    • Related Report
      2013 Final Research Report
  • [Journal Article] Composition of the blood supply in the sublingual and submandibular spaces and its relationship to the lateral lingual foramen of the mandible.2014

    • Author(s)
      Nakajima K, Tagaya A, Otonari-Yamamoto M, Seki K, Araki K, Sano T, Okano T, Nakamura M
    • Journal Title

      Oral Surg Oral Med Oral Pathol Oral Radiol

      Volume: 117 Issue: 1 Pages: e32-e38

    • DOI

      10.1016/j.oooo.2012.03.032

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mast cell maturation is driven via a group III phospholipase A2-prostaglandin D2-DP1 receptor paracrine axis2013

    • Author(s)
      Taketomi Y, Ueno N, Kojima T, Sato H, Murase R, Yamamoto K, Tanaka S, Sakanaka M, Nakamura M, Nishito Y, Kawana M, Kambe N, Ikeda K, Taguchi R, Nakamizo S, Kabashima K, Gelb MH, Arita M, Yokomizo T, Nakamura M, Watanabe K, Hirai H, Nakamura M, Okayama Y, Ra C, Aritake K, Urade Y, Morimoto K, Sugimoto Y, Shimizu T, Narumiya S, Hara S, Murakami M
    • Journal Title

      Nat Immunol

      Volume: 14 Pages: 554-563

    • Related Report
      2013 Final Research Report
  • [Journal Article] Mast cell maturation is driven via a novel group III phospholipase A2-prostaglandin D2-DP1 receptor paracrine axis.2013

    • Author(s)
      Taketomi, Y., Morimoto, K., Murakami, M., et al., 他30名
    • Journal Title

      Nature Immunol.

      Volume: (印刷中) Issue: 6 Pages: 554-63

    • DOI

      10.1038/ni.2586

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Calcified tissue formation of subcutaneously transplanted mouse dental pulp2012

    • Author(s)
      Ikeda-Isogai M, Ohtsuka T, Baba K, Nonaka N, Nakamura M
    • Journal Title

      Acta Histochem

      Volume: 114 Pages: 55-61

    • Related Report
      2013 Final Research Report
  • [Journal Article] The transient appearance of zipper-like actin superstructures during the fusion of osteoclasts2012

    • Author(s)
      Takito J, Nakamura M, Yoda M, Tohmonda T, Uchikawa S, Horiuchi K, Toyama Y, Chiba K
    • Journal Title

      J Cell Sci

      Volume: 125 Pages: 662-672

    • Related Report
      2013 Final Research Report
  • [Journal Article] The role of the junctional epithelium in periodontal innate defense and homeostasis2012

    • Author(s)
      Tsukamoto Y, Usui M. Yamamoto G, Takagi Y, Tachikawa T, Yamamoto M, Nakamura M
    • Journal Title

      J Periodont Res

      Volume: 47 Pages: 750-757

    • Related Report
      2013 Final Research Report
  • [Journal Article] Linked precursors via the zipper-like structure or the filopodium during the secondary fusion of osteoclasts2012

    • Author(s)
      Takito J, Nakamura M
    • Journal Title

      Communicative & Integrative Biology

      Volume: 5 Pages: 450-454

    • Related Report
      2013 Final Research Report
  • [Journal Article] Intranasal administration of PACAP : Uptake by brain and regional brain targeting with cyclodextrins2012

    • Author(s)
      Nonaka N, Farr SA, Nakamachi T, Morley JE, Nakamura M, Shioda S, Banks WA
    • Journal Title

      Peptide

      Volume: 36 Pages: 168-175

    • Related Report
      2013 Final Research Report
  • [Journal Article] Involvement of intestinal intraepithelial lymphocytes in turnover of intestinal epithelial cells -Morphological and functional alterations due to daily administration of FK506-2012

    • Author(s)
      Hidaka M, Nakamura M, Ohmichi Y, Itoh J, Fukuzawa K, Masuko T, Yagi H
    • Journal Title

      Cell Immunol

      Volume: 276 Pages: 124-133

    • Related Report
      2013 Final Research Report
  • [Journal Article] Calcified tissue formation of subcutaneously transplanted mouse dental pulp.2012

    • Author(s)
      Ikeda-Isogai M, Ohtsuka T, Baba K, Nonaka N, Nakamura M.
    • Journal Title

      Acta Histochem

      Volume: 114(1) Issue: 1 Pages: 55-61

    • DOI

      10.1016/j.acthis.2011.02.007

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Role of the junctional epithelium in periodontal innate defense and homeostasis.2012

    • Author(s)
      Tsukamoto Y, Usui M, Yamamoto G, Takagi Y, Tachikawa T, Yamamoto M, Nakamura M.
    • Journal Title

      J Periodontal Res

      Volume: 47(6) Issue: 6 Pages: 750-7

    • DOI

      10.1111/j.1600-0765.2012.01490.x

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] The role of macrophages for the disappearance of Meckel's cartilage during development in mice2011

    • Author(s)
      Tsuzurahara F, Soeta S, Kawawa T, Baba K, Nakamura M
    • Journal Title

      Acta Histochem

      Volume: 113 Pages: 194-200

    • Related Report
      2013 Final Research Report
  • [Journal Article] Kupffer cells support extramedullary erythropoiesis induced by nitrogen-containing bisphosphonate in splenectomized mice2011

    • Author(s)
      Otsuka H, Yagi H, Endo Y, Nonaka N, Nakamura M
    • Journal Title

      Cell Immunol

      Volume: 271 Pages: 197-204

    • Related Report
      2013 Final Research Report
  • [Journal Article] Roles of platelets and macrophages in the protective effects of lipopolysaccharide against concanavalin A-induced murine hepatitis2011

    • Author(s)
      Yu Z, Otsuka H, Yamaguchi K, Kuroishi T, Sasano T, Sugawara S, Nakamura M, Endo Y
    • Journal Title

      Biochim Biophys Acta

      Volume: 1812 Pages: 1069-1079

    • Related Report
      2013 Final Research Report
  • [Journal Article] In situ proliferation and differentiation of macrophages in dental pulp2011

    • Author(s)
      Iwasaki K, Otsuka H, Yanagisawa N, Hisamitsu H, Manabe A, Nonaka N, Nakamura M
    • Journal Title

      Cell Tissue Res

      Volume: 346 Pages: 99-109

    • Related Report
      2013 Final Research Report
  • [Journal Article] Effects of surface roughness and dimorphism on the adhesion of Candida albicans to the surface of resins: scanning electron microscope analyses of mode and number of adhesions: scanning electron microscope analyses of mode and number of adhesions

    • Author(s)
      Mayahara M, Kataoka R, Arimoto T, Tamaki Y, Yamaguchi N, Watanabe Y, Yamasaki Y, Miyazaki T
    • Journal Title

      J Investig Clin Dent

      Volume: (in press)

    • Related Report
      2013 Final Research Report
  • [Presentation] 成体マウスにおける胚子ヘモグロビンの再発現について2013

    • Author(s)
      大塚裕忠,中村雅典
    • Organizer
      第188回日本解剖学会総会・全国学術集会
    • Place of Presentation
      高松
    • Related Report
      2013 Final Research Report
  • [Presentation] 破骨細胞融合時に出現する細胞間アクチン超構造体2013

    • Author(s)
      瀧戸二郎,中村雅典
    • Organizer
      第118回日本解剖学会総会・全国学術集会
    • Place of Presentation
      高松
    • Related Report
      2013 Final Research Report
  • [Presentation] 加齢に伴うマウス唾液腺における構造機能変化の比較検討2013

    • Author(s)
      野中直子,中町智哉,塩田清二,中村雅典
    • Organizer
      第118回日本解剖学会総会・全国学術集会
    • Place of Presentation
      高松
    • Related Report
      2013 Final Research Report
  • [Presentation] Histological and immunohistochemical comparison of mouse salivary glands in aging2013

    • Author(s)
      Nonaka N, Nakamura M
    • Organizer
      91th International Association for Dental Research
    • Place of Presentation
      Seatlle
    • Related Report
      2013 Final Research Report
  • [Presentation] ウシ骨由来移植材の吸収過程の解析2013

    • Author(s)
      新井宏,栁澤伸彰,鈴木治,中村雅典
    • Organizer
      第55回歯科基礎医学会学術大会・総会
    • Place of Presentation
      岡山
    • Related Report
      2013 Annual Research Report
  • [Presentation] マウス血リンパ節様構造における赤血球造血について2012

    • Author(s)
      大塚裕忠,中村雅典
    • Organizer
      第117回日本解剖学会総会・全国学術集会
    • Related Report
      2013 Final Research Report
  • [Presentation] マウス唾液腺におけるPACAPおよびVIPレセプターの免疫組織化学的局在とPACAPおよびVIPの鼻投与による唾液分泌への効果2012

    • Author(s)
      野中直子,中町智哉,塩田清二,中村雅典
    • Organizer
      第117回日本解剖学会総会・全国学術集会
    • Place of Presentation
      山梨
    • Related Report
      2013 Final Research Report
  • [Presentation] 脊髄白質の評価法2012

    • Author(s)
      柴田昌和,後藤昇,後藤潤,野中直子,中村雅典,塩田清二
    • Organizer
      第117回日本解剖学会総会・全国学術集会
    • Place of Presentation
      山梨
    • Related Report
      2013 Final Research Report
  • [Presentation] Immuno- histochemical localization of PACAP and VIP receptors in major salivary glands and the effect of PACAP on saliva secretion in mice2012

    • Author(s)
      Nonaka N, Nakamura M
    • Organizer
      American Association of Anatomists (AAA) Annual Meeting at Experimental Biology 2012
    • Place of Presentation
      San Diego
    • Related Report
      2013 Final Research Report
  • [Presentation] 人体解剖学実習におけるグループダイナミックス評価の検討その12012

    • Author(s)
      野中直子、中島功、中村雅典、片岡竜太
    • Organizer
      第31回日本歯科医学教育学会総会
    • Place of Presentation
      岡山
    • Related Report
      2013 Final Research Report
  • [Presentation] 加齢に伴うマウス唾液腺におけるPACAPレセプター局在の解析2012

    • Author(s)
      野中直子,中村雅典
    • Organizer
      第54回歯科基礎医学会学術大会・総会
    • Place of Presentation
      郡山
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒト下顎骨における海綿骨を考慮した大規模動的解析2012

    • Author(s)
      中納治久,吉田美智,藤島昭宏,中島功,高野直樹,中村雅典,宮崎隆,槇慎太郎
    • Organizer
      第71回日本矯正歯科学会大会
    • Place of Presentation
      岩手
    • Related Report
      2013 Final Research Report
  • [Presentation] Induction of hemal node like structures in mice2012

    • Author(s)
      Otsuka H, Nakamura M
    • Organizer
      14th International Congress of Histochemistry and Cytochemistry Poster Program 8
    • Related Report
      2013 Final Research Report
  • [Presentation] Immunohistochemical localization of PACAP and VIP receptors in majorsalivary glands and the effect of them on saliva secretion in mice2012

    • Author(s)
      Matoba Y, Nonaka N, Imamura E, Nakamura M
    • Organizer
      10th Asian Congress on Oral and Maxillofacial Surgeons (ACOMS)
    • Place of Presentation
      Bali-Indonesia
    • Related Report
      2013 Final Research Report
  • [Presentation] Simultaneous detection of primitive and definitive erythropoiesis in adult mice2012

    • Author(s)
      Otsuka H, Nakamura M
    • Organizer
      第41回日本免疫学会総会・学術集会記録集
    • Related Report
      2013 Final Research Report
  • [Presentation] Immunohistochemical localization of pituitary adenylate cyclase-activating polypeptide (PACAP) and its receptor (PAC-1) in mouse major salivary glands2011

    • Author(s)
      Nonaka N,Nakamachi T,Shioda S,Nakamura M
    • Organizer
      AAA Annual Meeting at Experimental Biology 2011
    • Place of Presentation
      Washington DC
    • Related Report
      2013 Final Research Report
  • [Presentation] マウス唾液腺におけるPACAPレセプターの局在とPACAP経鼻投与による唾液分泌量の亢進2011

    • Author(s)
      野中直子,中町智哉,塩田清二,中村雅典
    • Organizer
      第11回日本抗加齢医学会総会
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report
  • [Presentation] マウスにおける血リンパ節様構造の形成誘導について2011

    • Author(s)
      大塚裕忠,中村雅典
    • Organizer
      第23回日本比較免疫学
    • Related Report
      2013 Final Research Report
  • [Presentation] マウス唾液腺におけるPACAPレセプターの局在とPACAPの唾液分泌への効果2011

    • Author(s)
      野中直子,中村雅典
    • Organizer
      第53回歯科基礎医学会学術大会ならびに総会
    • Place of Presentation
      岐阜
    • Related Report
      2013 Final Research Report
  • [Presentation] マウス唾液腺におけるPACAPレセプターおよびVIPレセプターの免疫組織化学的局在と唾液分泌への効果2011

    • Author(s)
      野中直子,中町智哉,塩田清二,中村雅典
    • Organizer
      第52回日本組織細胞化学会
    • Place of Presentation
      金沢
    • Related Report
      2013 Final Research Report
  • [Presentation] Immunohistochemical locali- zations of pituitary adenylate cyclase-activating polypeptide (PACAP) and its receptor (PAC1R) in mouse major salivary glands2011

    • Author(s)
      Nonaka N,Nakamachi T,Shioda S,Nakamura M
    • Organizer
      第10回日中合同組織細胞化学セミナー
    • Place of Presentation
      北京
    • Related Report
      2013 Final Research Report
  • [Presentation] Kupffer cells support hepatic erythropoiesis induced by nitrogen-containing bisphosphonate in splenectomized mice2011

    • Author(s)
      Otsuka H,Nakamura M
    • Organizer
      American College of Gastroenterology 76^<th> Annual Scientific Meeting
    • Related Report
      2013 Final Research Report
  • [Presentation] 舌下部とオトガイ下部の動脈ならびに下顎骨舌側に見られる小孔を通る動脈について2011

    • Author(s)
      中島功,中村雅典
    • Organizer
      第65回日本人類学会大会
    • Place of Presentation
      沖縄
    • Related Report
      2013 Final Research Report
  • [Presentation] マウスおよびヒト唾液腺におけるPACAPとPAC1Rの免疫組織化学的局在2011

    • Author(s)
      野中直子,中村雅典
    • Organizer
      第65回日本人類学会大会
    • Place of Presentation
      沖縄
    • Related Report
      2013 Final Research Report
  • [Book] The Functional Diversity of Kupffer Cells Handbook of Macrophage : Life Cycle, Functions and Diseases2013

    • Author(s)
      Nakamura M, Otsuka H, Yagi H, Yasuo Endo
    • Publisher
      NOVA science publishers
    • Related Report
      2013 Final Research Report
  • [Book] Extramedullary erythropoiesis in anemia.The New Frontiers in Research for Oral Cancer2013

    • Author(s)
      Nakamura M, Otsuka H, Nonaka N, Yanagisawa N, Yagi H, Endo Y
    • Publisher
      MARUZEN PLANET
    • Related Report
      2013 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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