Establishment of the newly bone regeneration therapy using polyhedra particles immobilized BMP-2 as a controlled release carrier for BMP-2

• Project/Area Number: 23593002
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Kanagawa Dental College
• Principal Investigator: OMI yasushi 神奈川歯科大学, 歯学部, 助教 (10318892)
• Co-Investigator(Kenkyū-buntansha): MATSUMOTO Goichi 神奈川歯科大学, 歯学部, 准教授 (60199867)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 骨再生治療 / バイオマテリアル / 多角体 / BMP-2 / 徐放 / アテロコラーゲンスポンジ / コラーゲンスポンジ / bone regeneration / polyhedra / DDS / 骨再生 / アテロコラーゲン

Research Abstract

Bombyx mori cypovirus particles are embedded in micrometer-sized protein crystals called polyhedra and can remain infectious in harsh environmental conditions for years. But the remarkable stability of polyhedra can be applied on slow-release carriers of cytokines for tissue engineering. Here we show the complete healing in critical-sized bone defects by bone morphogenetic protein-2 (BMP-2) encapsulated polyhedra. Although absorbable collagen sponge (ACS) safely and effectively delivers recombinant human BMP-2 (rhBMP-2) into healing tissue, the current therapeutic regimens release rhBMP-2 at an initially high rate after which the rate declines rapidly. ACS impregnated with BMP-2 polyhedra had enough osteogenic activity to promote complete healing in critical-sized bone defects, but ACS with a high dose of rhBMP-2 showed incomplete bone healing, indicating that polyhedral microcrystals containing BMP-2 promise to advance the state of the art of bone healing.

Research Products

• [Journal Article] Control of angiogenesis by VEGF and endostatin - encapsulated protein microcrystals and inhibition of tumor angiogenesis (2014)
  • Author(s): Matsumoto G, Hirohata R, Hayashi K, Sugimoto Y, Kotani E, Shimabukuro J, Hirano T, Nakajima Y, Kawamata S, Mori H
  • Journal Title: Biomaterials Volume: 35 Pages: 1326-1333
• [Journal Article] Control of angiogenesis by VEGF and endostatin - encapsulated protein microcrystals and inhibition of tumor angiogenesis. (2014)
  • Author(s): Matsumoto G, Hirohata R, Hayashi K, Sugimoto Y, Kotani E, Shimabukuro J, Hirano T, Nakajima Y, Kawamata S, Mori H.
  • Journal Title: Biomaterials Volume: 35 Pages: 1326-1333
  • Peer Reviewed
• [Journal Article] Gelatin powders accelerate the resorption of calcium phosphate cement and improve healing in the alveolar ridge (2013)
  • Author(s): Matsumoto G, Sugita Y, Kubo K, Yoshida W, Ikada Y, Sobajima S, Neo M, Maeda H, Kinoshita Y
  • Journal Title: J Biomater Appl Volume: 28 Pages: 1316-1324
• [Journal Article] Gelatin powders accelerate the resorption of calcium phosphate cement and improve healing in the alveolar ridge. (2013)
  • Author(s): Matsumoto G, Sugita Y, Kubo K, Yoshida W, Ikada Y, Sobajima S, Neo M, Maeda H, Kinoshita Y.
  • Journal Title: J Biomater Appl. Volume: 28 Pages: 1316-1324
  • Peer Reviewed
• [Journal Article] Bone regeneration by polyhedral microcrystals from silkworm virus. (2012)
  • Author(s): Matsumoto, G., Ueda, T., Shimoyama, J., Ijiri, H., Omi, Y., Yube, H., Sugita, Y., Kubo, K., Maeda, H., Kinoshita, Y., Arias, D. G.G., Shimabukuro, J., Kotani, E., Kawamata, S., and Mori, H.
  • Journal Title: Sci. Rep. Volume: 2巻 Issue: 1 Pages: 935-935
  • DOI: 10.1038/srep00935
  • Peer Reviewed
• [Presentation] ウイルス多角体を用いたBMP-2徐放による骨再生療法の開発 (2012)
  • Author(s): 松本剛一、夕部寿人、杉田好彦、久保勝彦、木下靭彦、前田初彦
  • Organizer: 日本口腔外科学会総会
  • Place of Presentation: 横浜
• [Presentation] BMP-2固定化多角体を用いた新規骨再生療法の開発 (2011)
  • Author(s): 松本剛一、下山淳子、久保田英朗、窪田展久、川股亮太、久保勝俊、木下靭彦、前田初彦
  • Organizer: 第65回日本口腔科学会学術集会
  • Place of Presentation: 東京
• [Remarks]
  • URL: http://www.nature.com/srep/2012/121206/srep00935/full/srep00935.html/
• [Remarks] Scientific Reports Article
  • URL: http://www.nature.com/srep/2012/121206/srep00935/full/srep00935.html