| Project/Area Number |
23593006
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Asahi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
DOI Yutaka 朝日大学, 歯学部, 名誉教授 (40116067)
SHIKIMORI Michio 朝日大学, 歯学部, 教授 (70154193)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 生体材料 / 再生医学 / 細胞・組織 / 炭酸含有アパタイト / 再生医療 |
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| Outline of Final Research Achievements |
In this study, our purpose was development of ideal biomaterial for bone substitution, and confirmation of that was replaced with bone.
Considering absorbability, we tried to make “non-sintered” porous carbonate apatite. The manufactured samples were examined by X-ray diffraction, FT-IR and micro focus CT, and confirmed those were containing several percent carbonate ion and approximately 56% porosity.
We cultured rat bone marrow cells with non-sintered porous carbonate apatite blocks as scaffold in CO2 incubator for two weeks, and transplanted it in rat dorsal subcutaneous site. Bone formation was observed in 2 weeks or later. This suggests that the non-sintered porous carbonate apatite has ability as scaffold for bone formation. We observed absorption of the sample due to osteoclasts with TRAP staining. TRAP positive reaction was almost observed near by bone formation. However in a part of the specimen in 8 weeks, TRAP positive reaction was observed out of bone formation site.
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