| Project/Area Number |
23593008
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Osaka Dental University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
DOMAE Naochika 大阪歯科大学, 歯学部, 名誉教授 (60115889)
KANEDA Kazuhiro 大阪歯科大学, 歯学部, 講師(非常勤) (90533886)
SUGIOKA Shingo 大阪歯科大学, 歯学部, 講師 (90278573)
KOTANI Junichiro 大阪歯科大学, 歯学部, 教授 (40109327)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | sevoflurane / heart / cardioprotection / autophagy / ischemia |
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| Research Abstract |
Sevoflurane preconditioning(SEVO-PC) is lost if the ischemic insult is too long. We examined whether induction of autophagy prolongs SEVO-PC protection during a longer ischemic insult.Isolated guinea pigs hearts were subjected to 30 or 45min ischemia,followed by 120min reperfusion. Anesthetic preconditioning was elicited with 2% sevoflurane for ten minutes prior to ischemia. Infarct size sparing effect of SEVO-PC was seen in 30min ischemia, but not 45min ischemia.Induction of autophagy by chloramphenicol prior to ischemia restored the infarct size limiting effect of SEVO-PC lost by 45min ischemia. Western blot analysis showed that expression of Akt and glycogen synthase kinase 3beta was enhanced by induction of autophagy.The effect of autophagy on calcium-induced mitochondrial permeability transition pore(MPTP) opening in isolated mitochondria from cardiomyocytes was assessed.The restoration of SEVO-PC lost by 45min ischemia is associated with enhanced inhibition of MPTP by autophagy.
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