| Project/Area Number |
23593014
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Asahi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KONDOH Nobuo 朝日大学, 歯学部口腔生化学分野, 教授 (40202072)
TAKAYA Eiji 朝日大学, 歯学部口腔生化学分野, 准教授 (70533446)
KAMIYA Masako 朝日大学, 歯学部口腔生化学分野, 助教 (80181907)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 創薬 / 再生医療 / 軟骨 / CCNファミリー / 肢芽 / 高密度培養 / ERK1/2 / MAPK / ERK / CCN2 / CCN3 / CTGF / NOV / 軟骨分化 / MAPK経路 / 遺伝子発現 |
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| Research Abstract |
CCN2 is best known as a promoter of chondrocyte proliferation and differentiation among the CCN family members. However, little is known concerning roles of the other CCN member, CCN3 in chondrocytes. These two proteins consists of 4 conserved modules that are highly interactive with a number of biomolecules. In the present study, we demonstrated that CCN3 protein promotes early chondrogenesis based on the following observations: 1) CCN3 was expressed in the embryonic limb mesenchymal cells, whereas, CCN2 was highly expressed in differentiated chondrocytes. 2) Exogenous recombinant CCN3 promoted the proliferation of limb mesenchymal cells in plane culture, and induced proteoglycan accumulation in micromass cultures. 3) By Ccn3 knockdown, chondrogenesis was inhibited in cultures. In addition, we found that extracellular signal-regulated kinase 1/2 pathway was involved in the CCN3-mediated modification of mesenchymal cell and chondroblast activities.
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