Novel pathogenicity of pili of oral streptococci

• Project/Area Number: 23593027
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Osaka University
• Principal Investigator: NOBUO Okahashi 大阪大学, 歯学研究科(研究院), 准教授 (40150180)
• Co-Investigator(Kenkyū-buntansha): OOSHIMA Takashi 大阪大学, 大学院歯学研究科, 教授 (80116003)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: pili / oral streptococci

Research Abstract

Streptococcus sanguinis is a major member of the oral streptococci. In this study, we identified a unique cell surface filamentous structure named pili. We found that S. sanguinis pili are involved in biofilm formation. Further, pili subunit proteins are shown to bind salivary proteins such as amylase and salivary mucin. Another type-2 pili was also found in S. sanguinis. Further study revealed that type-2 pili-expressing oral streptococci are common habitants in healthy human. These results suggest that pili of oral streptococci contribute to the bacterial colonization in the human oral cavity.

Research Products

• [Journal Article] Hydrogen peroxide contributes to the epithelial cell death induced by the oral mitis group of streptococci (2014)
  • Author(s): Okahashi N, Sumitomo T, Nakata M, Sakurai A, Kuwata H, Kawabata S
  • Journal Title: PLOS ONE Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Osteoprotegerin-deficient male mice as a model for severe alveolar bone loss : comparison with RANKL-overexpressing transgenic mice (2013)
  • Author(s): Koide M, Kobayashi Y, Ninomiya T, Yasuda H, Arai Y, Okahashi N, Yoshinari N, Takahashi N, Udagawa N
  • Journal Title: Endocrinol Volume: 154 Pages: 773-782
• [Journal Article] Hydrogen peroxide produced by oral streptococci induces macrophage cell death (2013)
  • Author(s): Okahashi N, Nakata M, Sumitomo T, Terao Y. Kawabata S
  • Journal Title: PLOS ONE Volume: 8
• [Journal Article] Distribution of amoxicillin- resistant oral streptococci in dental plaque specimens obtained from Japanese children and adolescents at risk for infective endocarditis (2013)
  • Author(s): Nemoto H, Nomura R, Ooshima T, Nakano K
  • Journal Title: J Cardiol Volume: 62 Pages: 296-300
• [Journal Article] Hydrogen peroxide produced by oral streptococci induces cell death of macrophage. (2013)
  • Author(s): Okahashi N, Nakata M, Sumitomo T, Terao Y, Kawabata S.
  • Journal Title: PLoS One Volume: 8 Issue: 5 Pages: 62563-62563
  • DOI: 10.1371/journal.pone.0062563
  • Peer Reviewed
• [Journal Article] Distribution of amoxicillin-resistant oral streptococci in dental plaque specimens obtained from Japanese children and adolescents at risk for infective endocarditis. (2013)
  • Author(s): Nemoto H, Nomura R, Ooshima T, Nakano K.
  • Journal Title: Journal of Cardiology Volume: 62 Issue: 5 Pages: 296-300
  • DOI: 10.1016/j.jjcc.2013.05.008
  • Peer Reviewed
• [Journal Article] Osteoprotegerin-deficient male mice as a model for severe alveolar bone loss: Comparison with RANKL-overexpressing transgenic male mice. (2013)
  • Author(s): Koide M et al.
  • Journal Title: Endocrinology Volume: 190 Issue: 2 Pages: 773-782
  • DOI: 10.1210/en.2012-1928
  • Peer Reviewed / Open Access
• [Journal Article] Hydrogen peroxide produced by oral streptococci induces macrophage cell death (2013)
  • Author(s): Okahashi N, Nakata M, Sumitomo T, Terao Y, Kawabata S.
  • Journal Title: PLoS One Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Infection of specific strains of Streptococcus mutans, oral bacteria, confers a risk of ulcerative colitis (2012)
  • Author(s): Kojima A, Nakano K, Wada K,(他10名), Ooshima T
  • Journal Title: Sci Rep Volume: 2
• [Journal Article] Complete genome sequence of the serotype k Streptococcus mutans strain LJ23 (2012)
  • Author(s): Aikawa C,(他10名), Ooshima T
  • Journal Title: J Bacteriol Volume: 194 Pages: 2754-2755
• [Journal Article] Infection of specific strains of Streptococcus mutans, oral bacteria, confers a risk of ulcerative colitis (2012)
  • Author(s): Kojima A, Nakano K, Wada K, Takahashi H, Katayama K, Yoneda M, Higurashi T, Nomura R, Hokamura K, Muranaka Y, Matsuhashi N, Umemura K, Kamisaki Y, Nakajima A, Ooshima T.
  • Journal Title: Scientific Reports Volume: 2 Issue: 1 Pages: 332-332
  • DOI: 10.1038/srep00332
  • Peer Reviewed
• [Journal Article] Complete genome sequence of the serotype k Strept ococcus mutans strain LJ23. (2012)
  • Author(s): Aikawa C, Furukawa N, Watanabe T, Minegishi K, Furukawa A, Eishi Y, Oshima K, Kurukawa K, Hattori M, Nakano K, Maruyama F, Nakagawa I, Ooshima T
  • Journal Title: Journal of Bacteriology Volume: 194 Issue: 10 Pages: 2754-2755
  • DOI: 10.1128/jb.00350-12
  • Peer Reviewed
• [Journal Article] Novel Epitopic Region of Glucosyltransferase B from Streptococcus mutans (2012)
  • Author(s): Tomonori Hoshino, Yoshio Kondo, Kan Saito, Yutaka Terao, Nobuo Okahashi, Shigetada Kawabata, and Taku Fujiwara, Shigetada Kawabata, and Taku Fujiwara
  • Journal Title: Clinical and Vaccine Immunology Volume: 18(9) Issue: 9 Pages: 1552-1561
  • DOI: 10.1128/cvi.05041-11
  • NAID: 120006985025
  • Peer Reviewed
• [Journal Article] α-enolase of Streptococcus pneumoniae induces formation of neutrophil extracellular traps (2012)
  • Author(s): Mori Y., Yamaguchi M., Terao Y., Hamada S., Ooshima T., Kawabata S.
  • Journal Title: Journal of Biological Chemistry Volume: 287 Issue: 13 Pages: 10472-10481
  • DOI: 10.1074/jbc.m111.280321
  • Peer Reviewed
• [Journal Article] Streptococcus sanguinis induces foam cell formation and cell death in association with production of reactive oxygen species (2011)
  • Author(s): Okahashi N,(他7名), Ooshima T, Nishihara T
  • Journal Title: FEMS Lett Volume: 323 Pages: 164-170
• [Journal Article] Assembly mechanisim of FCT region of type I pili in serotype M6 Streptococcus pyogenes (2011)
  • Author(s): Nakata M,(他8名), Okahashi N, Hamada S, Isoda R, Terao Y, Kawabata S
  • Journal Title: J Biol Chem Volume: 286 Pages: 37566-37577
• [Journal Article] a-enolase of Streptococcus pneumoniae induced formation of neutrophil extracellular traps (2011)
  • Author(s): Mori Y, Yamaguchi M, Terao Y, Hamada S, Ooshima T, Kawabata S
  • Journal Title: J Biol Chem Volume: 287 Pages: 10472-10481
• [Journal Article] Novel epitopic region of glucosyltransferase from Streptococcus mutans (2011)
  • Author(s): Hoshino T, Kondo Y, Saito K, Terao Y, Okahashi N, Kawabata S, Fujiwara T
  • Journal Title: Clin Vaccine Immunol Volume: 18 Pages: 1552-1561
• [Journal Article] The collagen binding protein of Streptococcus mutans is involved in haemorrhagic stroke (2011)
  • Author(s): Nakano K,(他18名), Ooshima T
  • Journal Title: Nat Commun Volume: 2
• [Journal Article] Pili of oral Streptococcus sanguinis bind to salivary amylase and promote the biofilm formation (2011)
  • Author(s): Okahashi N, et al
  • Journal Title: Microbial Pathogenesis Volume: 50巻 Issue: 3-4 Pages: 148-154
  • DOI: 10.1016/j.micpath.2011.01.005
  • Peer Reviewed
• [Journal Article] Distribution of oral streptococci highly resistant to amoxicillin in dental plaque specimens from Japanese chlidren and adolescents (2011)
  • Author(s): Nemoto H, Nakano K, Masuda K, Wada K, Ardin AC, Nomura R, Ooshima T.
  • Journal Title: J Med Microbiol Volume: 60(12) Issue: 12 Pages: 1853-1859
  • DOI: 10.1099/jmm.0.027615-0
  • Peer Reviewed
• [Journal Article] The collagen-binding protein of Streptococcus mutans is involved in hemorrhagic stroke (2011)
  • Author(s): Nakano K, Hokamura K, Taniguchi N, Wada K, Kudo C, Nomura R, Kojima A, Naka S, Muranaka Y, Thura M, Nakajima A, Masuda K, Nakagawa I, Speziale P, Shimada N, Amano A, Kamisaki Y, Tanaka T, Umemura K, Ooshima T.
  • Journal Title: Nature Communications Volume: 2 Issue: 1 Pages: 485-485
  • DOI: 10.1038/ncomms1491
  • Peer Reviewed
• [Journal Article] Assembly mechanism of FCT region type 1 pili in serotype M6 Streptococcus pyogenes (2011)
  • Author(s): Nakata M, Kimura KR, Sumitomo T, Wada S, Sugauchi A, Oiki E, Higashino M, Kreikemeyer B, Podbielski A, Okahashi N, Hamada S, Isoda R, Terao Y, and Kawabata S
  • Journal Title: J Biol Chem Volume: 286 Issue: 43 Pages: 37566-37577
  • DOI: 10.1074/jbc.m111.239780
  • Peer Reviewed
• [Journal Article] Streptococcus sanguinis induces foam cell formation and cell death of macrophages in association with production of reactive oxygen species (2011)
  • Author(s): Okahashi N, et al
  • Journal Title: FEMS Microbiology Letters Volume: 323巻 Issue: 2 Pages: 164-170
  • DOI: 10.1111/j.1574-6968.2011.02375.x
  • Peer Reviewed
• [Presentation] 口腔レンサ球菌が産生する過酸化水素がマクロファージの細胞死を誘導する (2014)
  • Author(s): 岡橋暢夫、中田匡宣、住友倫子、寺尾豊、川端重忠
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: 東京
• [Presentation] 口腔レンサ球菌が産生する過酸化水素がマクロファージの細胞死を誘導する (2014)
  • Author(s): 岡橋暢夫、中田匡宣、住友倫子、寺尾豊、川端重忠
  • Organizer: 第８７回日本細菌学会総会
  • Place of Presentation: 東京都
• [Presentation] Streptococcus sanguinis が産生する細胞壁架橋型ヌクレアーゼによる免疫回避機構 (2014)
  • Author(s): 森田知里、住岡龍一、中田匡宣、住友倫子、岡橋暢夫、浜田茂幸、川端重忠
  • Organizer: 第８７回日本細菌学会総会
  • Place of Presentation: 東京都
• [Presentation] 過酸化水素は口腔レンサ球菌の隠れた病原因子かもしれない (2013)
  • Author(s): 岡橋暢夫、沖永敏則、桜井敦朗、寺尾豊、中田匡宣、川端重忠、西原達次
  • Organizer: 第55回歯科基礎医学会学術総会
  • Place of Presentation: 岡山市
• [Presentation] Streptococcus sanguinisはマクロファージの泡沫化と細胞死を誘導する (2013)
  • Author(s): 岡橋暢夫、沖永敏則、桜井敦朗、寺尾豊、中田匡宣、川端重忠、西原達次
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉市
• [Presentation] Streptococcus sanguinisの細胞架橋型ヌクレアーゼの機能解析 (2013)
  • Author(s): 森田知里、住岡龍一、中田匡宣、岡橋暢夫、本多真理子、住友倫子、川端重忠
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉市
• [Presentation] 過酸化水素は口腔レンサ球菌の隠れた病原因子かもしれない (2013)
  • Author(s): 岡橋暢夫、沖永敏則、桜井敦朗、寺尾豊、中田匡宣、川端重忠、西原達次
  • Organizer: 第５５回歯科基礎医学会学術総会
  • Place of Presentation: 岡山市
• [Presentation] S. sanguinis の菌体表層ヌクレアーゼは自然免疫からの回避に関与する (2013)
  • Author(s): 住岡龍一、森田知里、中田匡宣、岡橋暢夫、住友倫子、川端重忠
  • Organizer: 第５５回歯科基礎医学会学術総会
  • Place of Presentation: 岡山市
• [Presentation] Streptococcus sanguinis はマクロファージの泡沫化と細胞死を誘導する (2013)
  • Author(s): 岡橋暢夫、沖永敏則、桜井敦朗、寺尾豊、中田匡宣、川端重忠、西原達次
  • Organizer: 第８６回日本細菌学会総会
  • Place of Presentation: 千葉市
• [Presentation] Streptococcus sanguinis の細胞壁架橋型ヌクレアーゼの機能解析 (2013)
  • Author(s): 森田知里、住岡龍一、中田匡宣、岡橋暢夫、本多真理子、住友倫子、川端重忠
  • Organizer: 第８６回日本細菌学会総会
  • Place of Presentation: 千葉市
• [Presentation] Contribution of protein antigen to Streptococcus mutans-induced cerebral hemorrhage deterioraction (2012)
  • Author(s): Nomura R, Nakano K, Naka S, Ooshima T
  • Organizer: 90th General Session of the International Association for Dental Research
  • Place of Presentation: Iguacu Falls, Brazil
• [Presentation] ウーロン茶によるデンタルプラーク沈着抑制効果の検証 (2012)
  • Author(s): 永山佳代子、稲垣暁子、藤田一世、高島由紀子、 Ardin AC, 仲野道代、大嶋隆
  • Organizer: 第５０回日本小児歯科学会
  • Place of Presentation: 東京
• [Presentation] Contribution of protein antigen to Streptococcus mutans-induced cerebral hemorrhage deterioration. (2012)
  • Author(s): Nomura R, Nakano K, Naka S, Ooshima T.
  • Organizer: 90th General Session of the IADR
  • Place of Presentation: Iguacu Falls, Brazil
• [Presentation] T6 線毛の組立て機構の解析 (2012)
  • Author(s): 中田匡宣、住友倫子、東野美晴、岡橋暢夫、浜田茂幸、寺尾豊、川端重忠
  • Organizer: 第８５回日本細菌学会総会
  • Place of Presentation: 長崎
• [Presentation] Pili of oral Streptococcus sanguinis bind to salivary amylase and promotes biofilm formation (2011)
  • Author(s): Okahashi N, Nakata M, Terao Y, Isoda R, Sakurai A, Sumitomo T, Yamaguchi M, Kimura KR, Oiki E, Kawabata S, Ooshima T
  • Organizer: XIII International Congress of Bacteriology and Applied Microbiology
  • Place of Presentation: Sapporo, Japan
• [Presentation] Distribution of Streptococcus mutans strains with collagen-binding properties (2011)
  • Author(s): Naka S, Nakano K, Nomura R, Ooshima T
  • Organizer: 23rd Meeting of International Association of Pediatric Dentistry
  • Place of Presentation: Athens, Greece
• [Presentation] Distribution of Streptococcus mutans strains with collagen-binding properties (2011)
  • Author(s): Naka S, Nakano K, Nomura R, Ooshima T
  • Organizer: 23rd meeting of IAPD (International Association of Paediatric Dentistry)
  • Place of Presentation: Athens, Greece
• [Presentation] Pili of oral Streptococcus sanguinis bind to salivary amylase and promote biofilm formation (2011)
  • Author(s): Okahashi N, Nakata M, Terao Y, Isoda R, Sakurai A, Sumitomo T, Yamaguchi M, Kimura KR, Oiki E,Kawabata S, Ooshima T
  • Organizer: XIII International Congress of Bacteriology and Applied Microbiology
  • Place of Presentation: Sapporo, Japan