Effects of early life stress on transcription or translation of developmental brain nerve cells

• Project/Area Number: 23593031
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Hiroshima University
• Principal Investigator: MITSUHATA Chieko 広島大学, 医歯薬保健学研究院(歯), 助教 (10335664)
• Co-Investigator(Kenkyū-buntansha): KOZAI Katsuyuki 広島大学, 大学院医歯薬保健学研究院, 教授 (10178212) ; MORITA Katsuya 広島文化学園大学, 看護学部, 教授 (10116684)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 発達 / ストレス / 自閉症 / 幼若期ストレス / 脳の発達

Research Abstract

It is not clear about mechanism of pathogenic mechanism of mental disorder including autism. The purpose of this study is to elucidate the relation between early stress exposure and epigenetic gene regulations in the brain. It is also another purpose to explore the possibility that these epigenetic gene regulations exert influence to growth of brain development.There are some reports that early life events can induce long lasting changes in physiology and behavior.We chose Mother infant separation as early life stress.This separation starts from one day after birth for one week or two weeks.Corticosteron in the blood was measured. There was no significant difference between two groups.After this, we started another separation and total RNA samples of mesencephalon were prepared in seventh day after birth.New target genes were explored with DNA methylation and micro array. We did not get unique target genes.