Molecular mechanism of osteoclastic bone resorption by glucocorticoid-induced osteoporosis in childhood.
| Project/Area Number |
23593039
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
MAKI kenshi 九州歯科大学, 歯学部, 教授 (60209400)
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| Co-Investigator(Kenkyū-buntansha) |
EIJIRO Jimi 九州歯科大学, 歯学部, 教授 (40276598)
HIDEFUMI Fukushima 九州歯科大学, 歯学部, 助教 (70412624)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 破骨細胞 / c－Src / p130Cas / アクチン / 大理石骨病 / ステロイド / 顎骨 / c-Src / Pyk2 / インテグリン / osteoclast / Bone resorption / Actin cytoskeleton |
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| Research Abstract |
To investigate the role of p130Cas on osteoclastic bone resorption, we generated osteoclast-specific p130Cas conditional knockout (p130CasOCL-) mice. p130CasOCL- mice exhibit a high bone mass phenotype caused by defect in multinucleation and cytoskeleton organization causing bone resorption deficiency. Bone marrow cells from p130CasOCL- mice were able to differentiate into osteoclasts in vitro. However, osteoclasts from p130CasOCL- mice failed to form actin rings and resorb pits on dentine slices. Although the initial events of osteoclast attachment, such as ntegrin or Src phosphorylation, were intact, the Rac1 activity that organizes the actin cytoskeleton was reduced, and its distribution was disrupted in p130CasOCL- osteoclasts. Dock5, a Rho family guanine nucleotide exchanger, failed to associate with Src or Pyk2 in osteoclasts in the absence of p130Cas. These results strongly indicate that p130Cas plays pivotal roles in osteoclastic bone resorption.
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Report
(4 results)
Research Products
(38 results)
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[Journal Article] p130Cas plays important roles in osteoclastic bone resorption2013
Author(s)
Nagai Y, Osawa K, Fukushima H, Tamura Y, Aoki K, Ohya K, Yasuda H, Hikiji H, Takahashi M, Seta Y, Seo S, Kurokawa M, Kato S, Honda H, Nakamura I, Maki K, Jimi E
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Journal Title
J Bone Miner Res
Volume: 28
Pages: 2449-62
Related Report
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[Journal Article] Disruption of NF-κB1 prevents bone loss caused by mechanical unloading2013
Author(s)
Nakamura H, Aoki K, Masuda W, Alles N, Nagano K, Fukushima H, Osawa K, Yasuda H, Nakamura I, Mikuni-Takagaki Y, Ohya K, Maki K, Jimi E
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Journal Title
J Bone Miner Res
Volume: 28
Pages: 1457-67
NAID
Related Report
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[Journal Article] Disruption of NF-κB1 prevents bone loss caused by mechanical unloading.2013
Author(s)
Nakamura H, Aoki K, Masuda W, Alles N, Nagano K, Fukushima H, Osawa K, Yasuda H, Nakamura I, Mikuni-Takagaki Y, Ohya K, Maki K, Jimi E.
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Journal Title
J Bone Miner Res.
Volume: 28
Issue: 6
Pages: 1457-1467
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] p130Cas plays important roles in osteoclastic bone resorption2013
Author(s)
Nagai Y, Osawa k, Fukushima H, Tamura Y, Aoki K, Ohya K, Yasuda H, Hikiji H, Kato S, Honda H, Nakamura I, Maki K, Jimi E
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Journal Title
J. Bone Miner. Res
Volume: 28
Issue: 12
Pages: 2449-2462
DOI
Related Report
Peer Reviewed
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[Presentation] p130Casは破骨細胞の機能発現に関与する
Author(s)
永井香絵,大澤賢次,福島秀文,保田尚孝,田村幸彦,青木和広,大谷啓一,加藤茂明,牧憲司,仲村一郎,自見英治郎
Organizer
日本骨代謝学会学術大会
Place of Presentation
京王プラザホテル,東京
Related Report
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