| Project/Area Number |
23593048
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KARIBE Hiroyuki 日本歯科大学, 生命歯学部, 教授 (50234000)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 歯根形成 / ヘルトヴィッヒ上皮鞘 / 抗腫瘍薬 / シクロフォスファミド / 小児 / マウス |
|---|
| Research Abstract |
Recently,late effects of antineoplastic treatment are frequently observed in childhood cancer patients. Cyclophosphamide (CY) is one of the cytostatic drugs commonly used for cancer treatment in children. This study aimed to evaluate the effect of CY on dental development in mice. We treated 12-day-old ICR mice with CY (100 mg/kg, i.p.). Soft X-ray radiographs were obtained in lateral projection. The root / crown length (R/C) ratio of the first molar was calculated. Serial sagittal sections were prepared from the first molars and histomorphological and immunohistochemical (cytokeratin) studies were performed. At 27 days, the root length in the experimental group was approximately 60% of that in the control group. The Hertwig's epithelial root sheath in the experimental group disappeared from 20 days onwards. Our results indicate that CY can induce a defect in and alter the function of HERS and disruption of the epithelial sheath inhibits proliferation and root formation.
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