| Project/Area Number |
23593050
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Kanagawa Dental College |
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Principal Investigator |
YAMAUCHI Masato 神奈川歯科大学, 歯学研究科(研究院), 助教 (30230311)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Masahiro 東北大学, 歯学研究科(研究院), 教授 (40215562)
松澤 光洋 神奈川歯科大学, 歯学部, その他 (60288082)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 骨シアロ蛋白質 / Ｒｕｎｘ２ / ＢＭＰ－２ / Runx2 / 転写制御 / クロマチン / BMP2 / TGF-β1 / アメロジェニン / ヘルトウィッヒの上皮鞘(HERS)由来細胞 |
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| Research Abstract |
Bone Sialoprotein(BSP) is a tissue-specific marker of cementblast cells which has shown to be transformed from Hertwig's epithlial cells.To elucidate the mechanisms of gene transcription,3 putative osteoblast specific element 2 consensus motif(OSE2-1~OSE2-3) were investigated the ability of Runx2 to interact with cognate cis-acting elements. Forced expression of Runx2 induced 14 fold enhancement of transcription activity observed with the 6 x tandem repeats of OSE2-2 and 3 fold enhancement with that of OSE2-3, whereas 60% significant reduction was observed with that of OSE2-1. Chromatin immunorecipitation analyses revealed Runx2 were significantly higher recruited to OSE2-2 than to other OSE2s in vivo. BMP-2 increased Runx2 binding to these OSE2s and its transcription, whereas obvious reduction of OSE2-2 transcription induced by TGF-beta 1. These data suggested that the coordinated regulation of chromatin on respective OSE2s with Runx2 were modulated by TGF-beta/BMP signals.
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