Molecular genetic study of the specific tooth agenesis in the third molar of EL mice

• Project/Area Number: 23593053
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Tsurumi University
• Principal Investigator: ASADA Yoshinobu 鶴見大学, 歯学部, 教授 (20184145)
• Co-Investigator(Kenkyū-buntansha): IDAIRA Yayoi 鶴見大学, 歯学部, 講師 (40200018)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 歯胚消失 / ELマウス / 第三臼歯 / マイクロアレイ解析 / 遺伝発現解析 / Lef１遺伝子 / Shh遺伝子 / LAMA5遺伝子 / インテグリン受容体

Research Abstract

A detailed study using Real time PCR, in situ hybridization, immunohistological analysis and DNA microarray revealed that the absence of third molars in EL/sea mice is strongly controlled by the interaction between beta 1 integrin and laminin alpha 5. These results suggest that Apoptosis and Hedgehog pathways are associated with the development of the tooth germs of M3 in the bud stage of EL mice.
We concluded that the specific tooth agenesis in EL/sea mice is regulated by the basement membrane proteins such as beta 1 integrin and laminin alpha 5.

Research Products

• [Presentation] 歯種特異的な歯胚消失メカニズムの解明に向けて (2013)
  • Author(s): 成山明具美、伊平弥生、宗正隆明、朝田芳信
  • Organizer: 第51回日本小児歯科学会学術大会
  • Place of Presentation: 岐阜県
• [Presentation] 歯種特異的な歯胚消失メカニズムの解明に向けて
  • Author(s): 成山明具美
  • Organizer: 日本小児歯科学会
  • Place of Presentation: 長良川国際会議場、岐阜県
• [Presentation] Molecular genetic study of specific missing in the third molar of EL mice
  • Author(s): 成山明具美、伊平弥生、宗正隆明、朝田芳信
  • Organizer: 日本小児歯科学会
  • Place of Presentation: 長良川国際会議場(岐阜県)