| Project/Area Number |
23593065
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
OZAKI Yukio 長崎大学, 医歯(薬)学総合研究科, 助教 (60204187)
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| Co-Investigator(Kenkyū-buntansha) |
HARA Yoshitaka 長崎大学, 医歯(薬)学総合研究科, 教授 (60159100)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | Th17 / IL-17 / TLR2 / TLR4 / TNF-α / Th1 / IL-10 / 免疫組織化学 / IL-23 / Th 17 / 骨吸収 / Th2 / TLR / IFN-γ / IL-4 |
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| Research Abstract |
We injected peptidoglycan (PGN) as TLR(Toll-like receptor)2 ligand and/or lipopolysaccaride (LPS) as TLR4 ligand into the gingiva in mice lower jaw. Then, the samples of gingival tissue were made with paraffin. We immunostained them with anti-tumor necrosis factor alpha (TNF-alpha) , anti-interleukin 10 (IL-10) and anti-interleukin(IL)-17. Next, We counted the TNF-alpha , IL-10 and IL-17 positive cells in them. The number of TNF-alpha and IL-10 positive cells was the most numerous in samples stimulated by the both TLR2 and 4. The samples stimulated by single TLR had less positive cells. In case of IL-17, the samples stimulated by TLR2 expressed most positive cells and the samples stimulated by TLR4 had few positive cells. Interestingly, we found the sample stimulated by both ligands also had few positive cells. These results may show that accumulation of Th17 inhibited by the other T cell subsets, for example Th1, when the mouse gingival tissues were stimulated by PGN+LPS or LPS.
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