Examination of the effects of periodontitis on aging, and development of novel periodontal treatments

• Project/Area Number: 23593069
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Health Sciences University of Hokkaido
• Principal Investigator: NAGASAWA Toshiyuki 北海道医療大学, 歯学部, 准教授 (90262203)
• Co-Investigator(Kenkyū-buntansha): TSUJI Masahiro 北海道医療大学, 教授 (70364271)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: Klotho / 老化 / 歯周炎 / IL-6 / gingival fibroblast / 歯周病 / 糖尿病 / FOXO / HbA1c / Diabetes / Periodontitis / Insulin

Research Abstract

The purpose of this study was to examine the effects of periodontitis on aging. Periodontitis patients with type 2 diabetes were examined to evaluate the association between inflammation and vascular aging. Periodontal tissue of Klotho mouse was examined to evaluate the association between accelerated aging and periodontitis. Results suggested that inflammation might enhance atherosclerosis through the rapid fluctuation of blood sugar. In addition, gingival fibroblasts from Klotho mouse had reduced growth rate, but enhanced inflammatory responses. These results suggest that periodontitis might affect aging through the systemic inflammation.

Research Products

• [Journal Article] Host-microbial co-evolution in periodontitis associated with Aggregatibacter actinomycetemcomitans infection (2014)
  • Author(s): Nagasawa T, Shimizu S, Kato S, Nakatsuka Y, Kado T, Hidaka T, Shirai K, Mori M, Furuichi Y
  • Journal Title: J Oral Biosci Volume: 56 Pages: 11-17
• [Journal Article] Host microbial co-evolution in periodontitis associated with Aggregatibacter actinomycetemcomitans infection (2014)
  • Author(s): Nagasawa T, Shimizu S, Kato S, Nakatsuka Y, Kado T, Hidaka T, Shirai K, Mori M, Furuichi Y
  • Journal Title: J Oral Biosci Volume: 56 Pages: 11-17
  • Peer Reviewed
• [Journal Article] Effect of glycemic control on periodontitis in type 2 diabetic patients with periodontal disease (2013)
  • Author(s): Katagiri S, Nitta H, Nagasawa T, Izumi Y, Kanazawa M, Matsuo A, Chiba H, Fukui M, Nakamura N, Oseko F, Kanamura N, Inagaki K, Noguchi T, Naruse K, Matsubara T, Miyazaki S, Miyauchi T, Ando Y, Hanada N, Inoue S
  • Journal Title: J Diabetes Investig Volume: 4(3) Pages: 320-325
• [Journal Article] Periodontal treatment with topical antibiotics improves glycemic control in association with elevated serum adiponectin in patients with type 2 diabetes mellitus (2013)
  • Author(s): Bharti P, Katagiri S, Nitta H, Nagasawa T, Kobayashi H, Takeuchi Y, Izumiyama H, Uchimura H, Inoue S, Izumi Y
  • Journal Title: Obesity Research and Clinical Practice Volume: 7(2)
• [Journal Article] The anti-phospholipid antibody-dependent and independent effects of periodontopathic bacteria on threatened preterm labor and preterm birth Archives of Gynecology and Obstetrics (2013)
  • Author(s): Ye CC, Katagiri S, Miyasaka N, Bharti P, Kobayashi H, Takeuchi Y, Momohara Y, Sekiguchi M, Takamine S, Nagasawa T, and Izumi Y
  • Volume: 288(1)
  • Pages: 65-72
• [Journal Article] Induction of Toll-Like Receptor Expression by Porphyromonas gingivalis (2013)
  • Author(s): Wara-aswapati N, Chayasadom A, Surari R, Pitiphat W, Boch JA, Nagasawa T, Ishikawa I, and Izumi Y
  • Journal Title: J Periodontol Volume: 84(7) Pages: 1010-1018
• [Journal Article] Involvement of Toll-like receptor 2 in apoptosis of Aggregatibacter actinomycetemcomitans-infected THP-1 cells (2013)
  • Author(s): Kato S, Nakashima K, Nagasawa T, Abiko Y, Furuichi Y
  • Journal Title: J Microbiol Immunol Infect Volume: 46(3) Pages: 64-70
• [Journal Article] The anti-phospholipid antibody-dependent and independent effects of periodontopathic bacteria on threatened preterm labor and preterm birth (2013)
  • Author(s): Ye CC, Katagiri S, Miyasaka N, Bharti P, Kobayashi H, Takeuchi Y, Momohara Y, Sekiguchi M, Takamine S, Nagasawa T, and Izumi Y
  • Journal Title: Archives of Gynecology and Obstetrics Volume: 288 Issue: 1 Pages: 65-72
  • DOI: 10.1007/s00404-013-2741-z
  • Peer Reviewed
• [Journal Article] Effect of glycemic control on periodontitis in type 2 diabetic patients with periodontal disease. (2013)
  • Author(s): Katagiri S, Nitta H, Nagasawa T, Izumi Y, Kanazawa M, Matsuo A, Chiba H, Fukui M, Nakamura N, Oseko F, Kanamura N, Inagaki K, Noguchi T, Naruse K, Matsubara T, Miyazaki S, Miyauchi T, Ando Y, Hanada N, Inoue S.
  • Journal Title: J Diabetes Investig. Volume: 4 Pages: 320-325
  • Peer Reviewed
• [Journal Article] Improvement of glycemic control after periodontal treatment by resolving gingival inflammation in type 2 diabetic patients with periodontal disease (2012)
  • Author(s): Katagiri S, Nagasawa T, Kobayashi H, Takamatsu H, Bharti P, Izumiyama H, Uchimura I, Tagami T, Suzuki T, Nanbara H, Taniguchi Y, Hayakumo S, Koyanagi T, Himeno-Ando A, Goto M, Kajio H, Takahashi Y, Izumi Y, Noda M
  • Journal Title: J Diabetes Invest Volume: 3 Pages: 402-409
• [Journal Article] Modulation of wnt5a expression by periodontopathic bacteria (2012)
  • Author(s): Nanbara H, Wara-Aswapati N, Nagasawa T, Yoshida Y, Yashiro R, Bando Y, Kobayashi H, Khongcharoensuk J, Hormdee D, Pitiphat W, Boch JA, Izumi Y
  • Journal Title: PLoS One Volume: 7(4)
• [Journal Article] FGF-2 induces proliferation of human periodontal ligament cells and maintains differentiation potentials of STRO-1+/CD146+ periodontal ligament cells (2012)
  • Author(s): Hidaka T, Nagasawa T, Shirai K, Kado T, Furuichi Y
  • Journal Title: Archs oral Biol(e pub ahead of printing) Volume: 57(6) Pages: 830-840
• [Journal Article] Salivary levels of antibacterial peptide (LL-37/hCAP-18) and cotinine in periodontitis patients (2012)
  • Author(s): Takeuchi Y, Nagasawa T, Katagiri S, Kitagawara S, Kobayashi H, Koyanagi T, Izumi Y
  • Journal Title: J Periodontol Volume: 83(6) Pages: 766-772
• [Journal Article] Salivary levels of antibacterial peptide (LL-37/hCAP-18) and cotinine in periodontitis patients (2012)
  • Author(s): Takeuchi Y, Nagasawa T, Katagiri S, Kitagawara S, Kobayashi H, Koyanagi T, Izumi Y.
  • Journal Title: J Periodontol Volume: 83 Pages: 766-72
  • Peer Reviewed
• [Journal Article] Modulation of Wnt5a expression by periodontopathic bactreia (2012)
  • Author(s): 南原弘美、和泉雄一, ら
  • Journal Title: Plos ONE Volume: 7 Issue: 4 Pages: e34434-e34434
  • DOI: 10.1371/journal.pone.0034434
  • Peer Reviewed
• [Journal Article] Modulation of wnt5a expression by periodontopathic bacteria. (2012)
  • Author(s): 39.Nanbara H, Wara-Aswapati N, Nagasawa T, Yoshida Y, Yashiro R, Bando Y, Kobayashi H, Khongcharoensuk J, Hormdee D, Pitiphat W, Boch JA, Izumi Y.
  • Journal Title: PLoS One. Volume: 7
  • Peer Reviewed
• [Journal Article] Reduced masticatory function in non-elderly obese Japanese adults (2011)
  • Author(s): Katagiri S, Nitta H, Nagasawa T, Izumi Y, Kanazawa M, Matsuo A, Chiba H, Miyazaki S, Miyauchi T, Nakamura N, Oseko F, Kanamura N, Ando Y, Hanada N, Inoue S
  • Journal Title: Obesity Research and Clinical Practice Volume: 5
• [Presentation] 歯周病と糖尿病 (2014)
  • Author(s): 長澤敏行
  • Organizer: 第48回糖尿病学の進歩札幌コンベンションセンター
  • Place of Presentation: 札幌
  • Year and Date: 2014-03-07
• [Presentation] De novo diagnosis and treatment of periodontitis associated with Aggregatibacter actinomycetemcomitans infection (2013)
  • Author(s): Nagasawa T
  • Organizer: The 36^<th> Annual Scientific Meeting & 19^<th> General Assembly of Association for Dental Sciences of Republic of China
  • Place of Presentation: Taipei International Convention Center, Taipei
  • Year and Date: 2013-12-22
• [Presentation] Cross sectional study on the relationship between serum biochemical examination and periodontal parameters (2013)
  • Author(s): Nagasawa T, Terada Y, Konishi Y, Uehara O, Ueyonahara T, Maida T, Kawakami T, Tsuji M, Furuichi Y
  • Organizer: 10^<th> Asian Pacific Society of Periodontology Meeting
  • Place of Presentation: Nara Prefectual New Public Hall, Nara
• [Presentation] 糖尿病と歯周病の関連性－地域における医科歯科連携調査の結果から－ (2013)
  • Author(s): 藤原 正、長澤敏行、妹尾智子、九津見紳一朗、川上智史、古市保志
  • Organizer: 第56回春季日本歯周病学会学術大会
  • Place of Presentation: タワーホール船堀
• [Presentation] Cross sectional study on the relationship between serum biochemical examination and periodontal parameters (2013)
  • Author(s): Nagasawa T, Terada Y, Konishi Y, Uehara O, Ueyonahara T, Maida T, Kawakami T, Tsuji M, Furuichi Y
  • Organizer: 10th Asian Pacific Society of Periodontology Meeting
  • Place of Presentation: Nara Prefectual New Public Hall
• [Presentation] Point of care system detecting salivary Porphyromonas gingivalis (2013)
  • Author(s): Kobayashi H, Machigashira R, Suda R, Nagasawa T, Tanaka Y, Yoshini T, Yamamoto M, Noguchi K, Furuichi Y, Izumi Y
  • Organizer: 10th Asian Pacific Society of Periodontology Meeting
  • Place of Presentation: Nara Prefectual New Public Hall
• [Presentation] Periopathogenic bacteria in oral plaque, saliva and placenta from preterm low birth weight cases (2013)
  • Author(s): Ye C, Kobayashi H, Katagiri S, Takeuchi Y, Nagasawa T, Izumi Y
  • Organizer: 10th Asian Pacific Society of Periodontology Meeting
  • Place of Presentation: Nara Prefectual New Public Hall
• [Presentation] De novo diagnosis and treatment of periodontitis associated with Aggregatibacter actinomycetemcomitans infection (2013)
  • Author(s): Nagasawa T.
  • Organizer: The 36th Annual Scientific Meeting & 19th General Assembly of Association for Dental Sciences of Republic of China
  • Place of Presentation: Taipei International Convention Center,
  • Invited
• [Presentation] Host-microbial co-evolution in periodontitis associated with Aggregatibacter actinomycetemcomitans (2012)
  • Author(s): Nagasawa T
  • Organizer: 歯科基礎医学会
  • Place of Presentation: 奥羽大学 郡山
  • Year and Date: 2012-09-15
• [Presentation] 歯周病の概要と歯周病と糖尿病の関わりー歯周病専門医の立場から (2012)
  • Author(s): 長澤敏行
  • Organizer: 糖尿病と歯周病に関する医科・歯科合同シンポジウム
  • Place of Presentation: 札幌グランドホテル 札幌
  • Year and Date: 2012-03-24
• [Presentation] 糖尿病・肥満患者における口腔に関する多施設疫学研究 (2012)
  • Author(s): 新田浩、片桐さやか、長澤敏行、和泉雄一、金沢真雄、松尾朗、千葉博茂、宮崎滋、宮内孝、大迫文重、金村成智、福井道明、中村直登、安藤雄一、花田信宏、井上修二
  • Organizer: 第22回日本歯科医学会総会
  • Place of Presentation: 大阪国際会議場 大阪
• [Presentation] 歯周炎に罹患した2型糖尿病患者における歯周病治療および糖尿病治療による多施設介入研究 (2012)
  • Author(s): 片桐さやか、新田浩、長澤敏行、泉山肇、内村功、野口俊英、菊池毅、稲垣幸司、松原達昭、成瀬佳子、金沢真雄、松尾朗、千葉博茂、大迫文重、金村成智、福井道明、中村直登、井上修二、和泉雄一
  • Organizer: 第22回日本歯科医学会総会
  • Place of Presentation: 大阪国際会議場 大阪
• [Presentation] 歯根膜由来細胞移植による組織再生に成長因子の併用が及ぼす影響 (2012)
  • Author(s): 日高竜宏、長澤敏行、白井要、上与那原朝秀、門貴司、古市保志
  • Organizer: 第22回日本歯科医学会総会
  • Place of Presentation: 大阪国際会議場 大阪
• [Presentation] JP-2 type Aggregatibacter actinomycetemcomitans characterization in Japanese periodontitis patients (2012)
  • Author(s): Nagasawa T, Kato S, Shimizu S, Hidaka T, Kado T, Mori M, Furuichi Y
  • Organizer: American Academy of Periodontology 98^<th> Annual meeting
  • Place of Presentation: Los Angels Convention Center, Los Angels
• [Presentation] Expression of CD14, CD16 and CD45 on monocytes from aggressive periodontitis patients Europerio 7 (2012)
  • Author(s): Bharti P, Katagiri S, Ye C, Kobayashi H, Takeuchi Y, Nagasawa T and Izumi Y
  • Organizer: European Federation of Periodontology
  • Place of Presentation: Vienna, Austria
• [Presentation] JP2型leukotoxin promoterを有するA.actinomycetemcomitans菌の解析 (2012)
  • Author(s): 清水伸太郎、長澤敏行、門貴司、小松寿明、日高竜宏、加藤さつき、森真理、古市保志
  • Organizer: 第55回春季日本歯周病学会
  • Place of Presentation: 札幌コンベンションセンター 札幌
• [Presentation] 北海道在住の日本人家族から分離されたJP2タイプのA.actinomycetemcomitansの特徴 (2012)
  • Author(s): 長澤敏行、清水伸太郎、加藤さつき、門貴司、日高竜宏、森真理、古市保志
  • Organizer: 第55回春季日本歯周病学会
  • Place of Presentation: 札幌コンベンションセンター 札幌
• [Presentation] 早産・低体重児出産における歯周病に起因したβ2glycoprotein-Iを認識する抗体の役割 (2012)
  • Author(s): 葉暢暢、片桐さやか、バルティパリクシャ、小林宏明、竹内康雄、長澤敏行、和泉雄一
  • Organizer: 第55回春季日本歯周病学会
  • Place of Presentation: 札幌コンベンションセンター 札幌
• [Presentation] 内科および歯科検査値の相関に関する横断研究 (2012)
  • Author(s): 寺田裕、長澤敏行、小西ゆみ子、藤井健男、辻ひふみ、坂本亜衣子、植原治、上輿那原朝秀、舞田健夫、川上智史、井出肇、辻昌宏、古市保志
  • Organizer: 第55回春季日本歯周病学会
  • Place of Presentation: 札幌コンベンションセンター 札幌
• [Presentation] The role of periodontal bacteria on antiphospholipid syndrome and preterm low birth weight (2012)
  • Author(s): Ye C, Katagiri S, Bharti P, Kobayashi H, Takeuchi Y, Nagasawa T, Izumi Y
  • Organizer: 日中歯科大会
  • Place of Presentation: Chengdu(成都)
• [Presentation] Host-microbial co-evolution in periodontitis associated with Aggregatibacter actinomycetemcomitans (2012)
  • Author(s): Nagasawa T.
  • Organizer: 歯科基礎医学会
  • Place of Presentation: 奥羽大学、郡山市、福島県
  • Invited
• [Presentation] 北海道在住の日本人家族から分離されたJP2タイプのA.actinomycetemcomitansの特徴 (2012)
  • Author(s): 長澤敏行、清水伸太郎、加藤さつき、門 貴司、日高竜宏、森 真理、古市保志
  • Organizer: 第55回春季日本歯周病学会
  • Place of Presentation: 札幌コンベンションセンター
• [Presentation] JP2型leukotoxin promoterを有するA.actinomycetemcomitans菌の解析 (2012)
  • Author(s): 清水伸太郎、長澤敏行、門 貴司、小松寿明、日高竜宏、加藤さつき、森 真理、 古市保志
  • Organizer: 第55回春季日本歯周病学会
  • Place of Presentation: 札幌コンベンションセンター
• [Presentation] The role of periodontal bacteria on antiphospholipid syndrome and preterm low birth weight (2012)
  • Author(s): Changchang Ye, Sayaka Katagiri, Pariksha Bharti, Hiroaki Kobayashi, Yasuo Takeuchi, Toshiyuki Nagasawa, Yuichi Izumi
  • Organizer: 日中歯科大会２０１２
  • Place of Presentation: Chengdu(成都)、中国
• [Presentation] Periodontal treatment with topical antibiotics improves glycemic control in association with elevated serum adiponectin in patients with type 2 diabetes mellitus (2012)
  • Author(s): Pariksha Bharti, Sayaka Katagiri, Hiroshi Nitta, Toshiyuki Nagasawa, Hiroaki Kobayashi, Yasuo Takeuchi, Hajime Izumiyama, Isao Uchimura, Shuji Inoue and Yuichi Izumi
  • Organizer: 2nd Tri-University Consortium on Oral Science and Education
  • Place of Presentation: Beijin、中国
• [Presentation] Iron acquisition of A.actinomycetemcomitans with JP2 type leukotoxin isolated from Japanese periodontitis patients in Hokkaido (2011)
  • Author(s): Nagasawa T, Kato S, Shimizu S, Hidaka T, Kado T, Mori M, Furuichi Y
  • Organizer: 9^<th> Asian Pacific Society of Periodontology Meeting
  • Place of Presentation: L'Hotel Nina et Convention Centre, Hong Kong
• [Presentation] JP2型leukotoxin promoterを有するA.actinomycetemcomitans菌の分離と分析 (2011)
  • Author(s): 長澤敏行、清水伸太郎、日高竜宏、門貴司、加藤幸紀、森真理、古市保志
  • Organizer: 第135回日本歯科保存学会秋季学術大会
  • Place of Presentation: 大阪国際交流センター 大阪
• [Presentation] 臨床計測値と唾液および歯肉縁下プラークから検出される歯周病原細菌との相関性 (2011)
  • Author(s): 清水伸太郎、長澤敏行、古市保志
  • Organizer: 第54回春季日本歯周病学会
  • Place of Presentation: 福岡国際会議場 福岡
• [Presentation] 高齢者における歯周組織と全身の健康状態との関連性 (2011)
  • Author(s): 衣笠裕紀、長澤敏行、白井要、粟倉あずさ、日高竜宏、古市保志
  • Organizer: 第54回春季日本歯周病学会
  • Place of Presentation: 福岡国際会議場 福岡
• [Presentation] ヒト単球系細胞THP-1における歯周病原細菌刺激によるWnt5a遺伝子発現について (2011)
  • Author(s): 南原弘美、Wara-aswapati Nawarat、長澤敏行、吉田安宏、矢代麗子、坂東由記子、小林宏明、Boch Jason、和泉雄一
  • Organizer: 第54回春季日本歯周病学会
  • Place of Presentation: 福岡国際会議場 福岡
• [Presentation] ヒト単球系細胞THP-1における歯周病原細菌刺激によるWnt5a遺伝子発現について (2011)
  • Author(s): 南原弘美、Wara-aswapati Nawarat、長澤敏行、吉田安宏、矢代麗子、坂東由記子、小林宏明、Boch Jason、和泉雄一
  • Organizer: 第54回春季日本歯周病学会
  • Place of Presentation: 福岡国際会議場
• [Presentation] 高齢者における歯周組織と全身の健康状態との関連性 (2011)
  • Author(s): 衣笠裕紀、長澤敏行、白井 要、粟倉あずさ、日高竜宏、古市保志
  • Organizer: 第54回春季日本歯周病学会
  • Place of Presentation: 福岡国際会議場
• [Presentation] 臨床計測値と唾液および歯肉縁下プラークから検出される歯周病原細菌との相関性 (2011)
  • Author(s): 清水伸太郎、長澤敏行、古市保志
  • Organizer: 第54回春季日本歯周病学会
  • Place of Presentation: 福岡国際会議場
• [Presentation] Iron acquisition of A.actinomycetemcomitans with JP2 type leukotoxin isolated from Japanese periodontitis patients in Hokkaido (2011)
  • Author(s): Nagasawa T, Kato S, Shimizu S, Hidaka T, Kado T, Mori M, Furuichi Y
  • Organizer: 9th Asian Pacific Society of Periodontology Meeting
  • Place of Presentation: Hong Kong