Investigation of the chronic pain mechanism in the maxillofacial area using index of CRH gene expression in hypothalamus

• Project/Area Number: 23593082
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Social dentistry
• Research Institution: Tohoku University
• Principal Investigator: KASAHARA Eriko 東北大学, 歯学研究科(研究院), 大学院非常勤講師 (50597325)
• Co-Investigator(Kenkyū-buntansha): SHOJI Noriaki 東北大学, 病院, 講師 (70250800) ; SASANO Takashi 東北大学, 歯学研究科(研究院), 教授 (10125560) ; ICHIKAWA Hiroyuki 東北大学, 歯学研究科(研究院), 教授 (20193435)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: CRH / 急性痛 / 慢性痛 / ストレス / c-fos

Research Abstract

Recently, patients having chronic pain in the maxillofacial region, atypical odontalgia and glossodynia are increasing, and this becomes a social problem. However, it is not clear the mechanism of chronic pain and method of treatment. The aim of this study is diagnosis of stress of pain and correct therapy. We considered that the pain is one of impression as stressor, and analyzed the influence of the stress on the hypothalamus-pituitary-adrenal(HPA) axis and paraventricular hypothalamic nucleus(PVN). In this study, we inject the solution of capsaicin at the tip of the rat tongue that is controlled by the mandibular nerve branched from the trigeminal nerve, and clear the change in PVN by acute pain stress and following nerve injury.It is provided that the c-fos gene transcription which is a neuronal exciting marker is increasing in both the hypothalamic PVN and the trigeminal ganglion.

Research Products

• [Journal Article] Prednisolone induces microglial activation in the subnucleus caudalis of the rat trigeminal sensory complex. (2014)
  • Author(s): Y. Endo,N.Shoji,Y.Shimada,E.Kasahara,M.Iikubo,T.Sato,T.Sasano,H.Ichikawa
  • Journal Title: Cell Mol Neurobiol Volume: 34 Issue: 1 Pages: 95-100
  • DOI: 10.1007/s10571-013-9990-z
  • Peer Reviewed
• [Journal Article] The distribution of transient receptor potential melastatin-8in the rat soft palate, epiglottis, and pharynx. (2013)
  • Author(s): Sato T, Fujita M, Kano M, Hosokawa H, Kondo T, Suzuki T, Kasahara E, Shoji N, Sasano T, Ichikawa H.
  • Journal Title: Cell Mol Neurobiol Volume: 33 Issue: 2 Pages: 161-5
  • DOI: 10.1007/s10571-012-9888-1
  • Peer Reviewed
• [Presentation] Clinical survey of dentinhypersensitivity-like toothache induced by steroid therapy (2012)
  • Author(s): Yu Endo, Masahiro Iikubo, Noriaki Shoji, Tomonori Ishii, Hideo Harigae, Maya Sakamoto, Takashi Sasano
  • Organizer: 41th American Association for Dental Research
  • Place of Presentation: アメリカTampa
  • Year and Date: 2012-03-24
• [Presentation] Clinical survey of dentinhypersensitivity-like toothache induced by steroid therapy (2012)
  • Author(s): Yu Endo, Masahiro Iikubo, Noriaki Shoji, Tomonori Ishii, Hideo Harigae, Maya Sakamoto, Takashi Sasano
  • Organizer: 41th American Association for Dental Research
  • Place of Presentation: Tampa, USA