| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
To establish preventive methods against periodontitis-derived bone resorption, we first examine the effects of BIX01294 and chaetocin, which are inhibitors of H3K9 methyltransferase, on RANKL-induced osteoclast differentiation. Both inhibitors dose-dependently inhibited RANKL-induced osteoclast differentiation of preosteoclast-like Raw264.7 cells. BIX01294 and chaetocin rescues RANKL-induced downregulation of osteoclast-differentiation-inhibitory gene expressions in a dose-dependent manner. However, siRNA for G9a did not inhibit RANKL-induced osteoclast differentiation, suggesting that the effects of BIX01294 on osteoclast differentiation are not derived from G9a inhibition. In addition, chaetocin dose dependently inhibited RANKL-induced downregulation of blimp1 expression, which is reportedly inhibits osteoclast differentiation inhibitory genes.
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