Development of highly efficient bioethanol production yeast by genetic engineering

• Project/Area Number: 23603003
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomass energy
• Research Institution: Kyoto University
• Principal Investigator: KODAKI TSUTOMU 京都大学, エネルギー理工学研究所, 准教授 (70170264)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: バイオエタノール / 酵母 / キシロース / タンパク質工学

Research Abstract

Since xylose is one of the major fermentable sugars present in lignocellulosic biomass, the efficient fermentation of xylose is required to develop economically viable processes for producing bioethanol. In this study, construction of the strictly NADPH dependent xylose reductase from Pichia stipitis was succeeded by site directed mutagenesis. The more efficient xylose fermentation and the decrease of xylitol excretion was observed by introducing the strictly NADPH dependent XR with the strictly NADP+ dependent XDH. Next, by introducing many putative genes of xylose transporters of P. stipitis, the XUT1 and SUT1 genes of Pichia stipitis were shown to be the most effective on xylose consumption. Furthermore, Overexpression of the enzymes involved in the pentose phosphate pathway was shown to have positive effects on xylose fermentation.

Research Products

• [Journal Article] Boost in bioethanol production using recombinant Saccharomyces cerevisiae with mutated strictly NADPH-dependent xylose reductase and NADP+-dependent xylitol dehydrogenase (2013)
  • Author(s): Khattab, S. M. R., Saimura, M., and Kodaki., T.
  • Journal Title: I Biotech. Volume: 165 Issue: 3-4 Pages: 153-156
  • DOI: 10.1016/j.jbiotec.2013.03.009
  • NAID: 120005245131
  • Peer Reviewed
• [Journal Article] Boost in Bioethanol Production using Recombinant Saccharomyces cerevisiae with Mutated Strictly NADPH-Dependent Xylose Reductase and NADP+-Dependent Xylitol Dehydrogenase (2013)
  • Author(s): Khattaba、S.M.R., Saimura, M. and Kodaki, T.
  • Journal Title: J. Biotech. Volume: 165 Pages: 153-156
  • NAID: 120005245131
  • Peer Reviewed
• [Journal Article] Effects of NADH-preferring xylose reductase expression on ethanol production from xylose in xylose-metabolizing recombinant Saccharomyces cerevisiae (2012)
  • Author(s): Lee, S-H., Kodaki, T., Park, Y-C., and Seo, J-H.
  • Journal Title: Ibiotech. Volume: 158 Issue: 4 Pages: 184-191
  • DOI: 10.1016/j.jbiotec.2011.06.005
  • Peer Reviewed
• [Journal Article] A novel strictly NADPH-dependent Pichia stipitis xylose reductase constructed by site-directed mutagenesis (2011)
  • Author(s): Khattab, S. M. R., Watanabe, S., Saimura, M., and Kodaki.
  • Journal Title: T. Biochem. Biophys. Res. Commun. Volume: 404 Issue: 2 Pages: 634-637
  • DOI: 10.1016/j.bbrc.2010.12.028
  • NAID: 120002753572
  • Peer Reviewed
• [Book] Green Energy and Technology "Zero-Carbon Energy Kyoto 2010" (2011)
  • Author(s): Khattab, S. M. R., Watanabe, S., Saimura, M., Afifi, M. M., Zohri, A.-N. A., Abdul-Raouf, U. M., and Kodaki, T.
  • Publisher: Construction of a novel strictly NADPH-dependent Pichia stipitis xylose reductase by site-directed mutagenesis for effective bioethanol production