| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
Since xylose is one of the major fermentable sugars present in lignocellulosic biomass, the efficient fermentation of xylose is required to develop economically viable processes for producing bioethanol. In this study, construction of the strictly NADPH dependent xylose reductase from Pichia stipitis was succeeded by site directed mutagenesis. The more efficient xylose fermentation and the decrease of xylitol excretion was observed by introducing the strictly NADPH dependent XR with the strictly NADP+ dependent XDH. Next, by introducing many putative genes of xylose transporters of P. stipitis, the XUT1 and SUT1 genes of Pichia stipitis were shown to be the most effective on xylose consumption. Furthermore, Overexpression of the enzymes involved in the pentose phosphate pathway was shown to have positive effects on xylose fermentation.
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