Basic research for biomass production using nitrogen-fixing cyanobacteria

• Project/Area Number: 23603005
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomass energy
• Research Institution: Tokyo Metropolitan University (2013); Chuo University (2011-2012)
• Principal Investigator: EHIRA Shigeki 首都大学東京, 理工学研究科, 准教授 (90548132)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: シアノバクテリア / バイオマス / 糖代謝 / 遺伝子発現制御 / バイオテクノロジー / ゲノム / 生体機能利用 / 応用微生物

Research Abstract

To develop the process of biological conversion of CO2 to fuel or chemicals by nitrogen-fixing cyanobacteria, we investigated the regulatory mechanisms of carbon metabolism, such as glycogen catabolism and sucrose synthesis in the nitrogen-fixing cyanobacterium Anabaena PCC 7120. We succeeded in identifying the regulators of carbon metabolism and altering carbon flux within cells by engineering of the regulators.

Research Products

• [Journal Article] NrrA directly regulates expression of the fraF gene and antisense RNAs for fraE in the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120 (2014)
  • Author(s): Ehira, S., Ohmori, M.
  • Journal Title: Microbiology Volume: 160 Pages: 844-850
  • Peer Reviewed
• [Journal Article] Attachment of phycobilisomes in an antenna-photosystem I supercomplex of cyanobacteria (2014)
  • Author(s): Watanabe, M., Semchonok, D.A., Webber-Birungi, M.T., Ehira, S., Kondo, K., Narikawa, R., Ohmori, M., Boekema, E.J., Ikeuchi, M.
  • Journal Title: Proc. Natl. Acad. Sci. USA. Volume: 111 Pages: 2512-2517
  • Peer Reviewed
• [Journal Article] Attachment of phycobilisomes in an antenna-photosystem I supercomplex of cyanobacteria (2014)
  • Author(s): Watanabe
  • Journal Title: Proc. Natl. Acad. Sci. U S A Volume: 111 Issue: 7 Pages: 2512-2517
  • DOI: 10.1073/pnas.1320599111
  • Peer Reviewed
• [Journal Article] Transcriptional regulation of heterocyst differentiation in Anabaena sp. strain PCC 7120 (2013)
  • Author(s): Ehira, S.
  • Journal Title: Russ. J. Plant Physiol. Volume: 60 Pages: 443-452
  • Peer Reviewed
• [Journal Article] Genome-wide and heterocyst-specific circadian gene expression in the filamentous cyanobacterium, Anabaena sp. PCC 7120 (2013)
  • Author(s): Kushige, H., Kugenuma, H., Matsuoka, M., Ehira, S., Ohmori, M., Iwasaki, H.
  • Journal Title: J. Bacteriol. Volume: 195 Pages: 1276-1284
  • Peer Reviewed
• [Journal Article] Genome-wide and heterocyst-specific circadian gene expression in the filamentous cyanobacterium, Anabaena sp. PCC 7120 (2013)
  • Author(s): Kushige, H., Kugenuma, H., Matsuoka, M., Ehira, S., Ohmori, M., Iwasaki, H.
  • Journal Title: Journal of Bacteriology Volume: 195 Issue: 6 Pages: 1276-1284
  • DOI: 10.1128/jb.02067-12
  • Peer Reviewed
• [Journal Article] Transcriptional regulation of heterocyst differentiation in Anabaena sp. strain PCC 7120 (2013)
  • Author(s): Ehira, S.
  • Journal Title: Russian Journal of Plant Physiology Volume: 60 Issue: 4 Pages: 443-452
  • DOI: 10.1134/s1021443713040043
  • Peer Reviewed
• [Journal Article] The redox-sensing transcriptional regulator RexT controls expression of thioredoxin A2 in the cyanobacterium Anabaena sp. strain PCC 7120 (2012)
  • Author(s): Ehira, S., Ohmori, M.
  • Journal Title: J. Biol. Chem. Volume: 287 Pages: 40433-40440
  • Peer Reviewed
• [Journal Article] The pknH gene restrictively expressed in heterocysts is required for diazotrophic growth in the cyanobacterium Anabaena sp. strain PCC 7120 (2012)
  • Author(s): Ehira, S., Ohmori, M.
  • Journal Title: Microbiology Volume: 158 Pages: 1437-1443
  • Peer Reviewed
• [Journal Article] Coordinated regulation of gnd encoding 6-phosphogluconate dehydrogenase by two transcriptional regulators GntR1 and RamA in Corynebacterium glutamicum (2012)
  • Author(s): Tanaka, Y., Ehira, S., Teramoto, H., Inui, M., Yukawa, H.
  • Journal Title: J. Bacteriol. Volume: 194 Pages: 65247-6536
  • Peer Reviewed
• [Journal Article] The redox-sensing transcriptional regulator RexT controls expression of thioredoxin A2 in the cyanobacterium Anabaena sp. strain PCC 7120 (2012)
  • Author(s): Shigeki Ehira, Masayuki Ohmori
  • Journal Title: Journal of Biological Chemistry Volume: 287 Issue: 48 Pages: 40433-40440
  • DOI: 10.1074/jbc.m112.384206
  • Peer Reviewed
• [Journal Article] Coordinated regulation of gnd encoding 6-phosphogluconate dehydrogenase by two transcriptional regulators GntR1 and RamA in Corynebacterium glutamicum (2012)
  • Author(s): Tanaka, Y., S. Ehira, H. Teramoto, M. Inui, and H. Yukawa
  • Journal Title: Journal of Bacteriology Volume: 194 Issue: 23 Pages: 6527-6536
  • DOI: 10.1128/jb.01635-12
  • Peer Reviewed
• [Journal Article] シアノバクテリアにおける細胞分化にともなう代謝変換制御機構の解明 (2012)
  • Author(s): 得平茂樹
  • Journal Title: 日本ゲノム微生物学会ニュースレター Volume: 5 Pages: 3-4
• [Journal Article] シアノバクテリアにおいてチオレドキシンの転写を制御するレドックス応答性転写因子RexT (2012)
  • Author(s): 得平茂樹
  • Journal Title: 光合成研究 Volume: 22 Pages: 160-164
  • NAID: 40019553698
  • Peer Reviewed
• [Journal Article] NrrA, a nitrogen-regulated response regulator protein, controls glycogen catabolism in the nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120 (2011)
  • Author(s): Ehira, S., Ohmori, M.
  • Journal Title: J. Biol. Chem. Volume: 286 Pages: 38109-38114
  • Peer Reviewed
• [Journal Article] NrrA, a nitrogen-regulated response regulator protein, controls glycogen catabolism in the nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120. (2011)
  • Author(s): Ehira Shigeki, Ohmori Masayuki
  • Journal Title: Journal of Biological Chemistry Volume: 286 Issue: 44 Pages: 38109-38114
  • DOI: 10.1074/jbc.m111.289124
  • Peer Reviewed
• [Presentation] Regulation of sucrose synthesis in the nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120 (2014)
  • Author(s): 得平茂樹, 木村聡, 大森正之
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山
• [Presentation] The orphan response regulator NrrA controls glycogen catabolism in cyanobacteria (2014)
  • Author(s): 得平茂樹, 西山英里, 藤木耕平
  • Organizer: 第8回日本ゲノム微生物学会年会
  • Place of Presentation: 東京
• [Presentation] Regulation of sugar metabolism in the nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120 (2013)
  • Author(s): Ehira, S.
  • Organizer: 18^<th> International Congress on Nitrogen Fixation
  • Place of Presentation: Miyazaki, Japan
• [Presentation] シアノバクテリアにおいて糖代謝を制御するレスポンスレギュレーターNrrAの活性制御 (2013)
  • Author(s): 得平茂樹, 藤木耕平, 西山英里
  • Organizer: 第4回日本光合成学会年会
  • Place of Presentation: 名古屋
• [Presentation] シアノバクテリアにおけるレドックスによるチオレドキシン発現制御 (2013)
  • Author(s): 得平茂樹
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山
• [Presentation] 糸状性シアノバクテリアAnabaena sp. PCC 7120における塩ストレス応答の制御機構 (2013)
  • Author(s): 得平茂樹, 木村聡, 大森正之
  • Organizer: 第8回日本ゲノム微生物学会年会
  • Place of Presentation: 滋賀
• [Presentation] 糸状性シアノバクテリアAnabaena sp. PCC 7120における塩ストレス応答の制御機構 (2013)
  • Author(s): 得平茂樹，木村聡，大森正之
  • Organizer: 日本ゲノム微生物学会
  • Place of Presentation: 長浜バイオ大学（滋賀）
• [Presentation] シアノバクテリアにおけるレドックスによるチオレドキシン発現制御 (2013)
  • Author(s): 得平茂樹
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岡山大学（岡山）
• [Presentation] Regulation of glycogen catabolism in the nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120 (2012)
  • Author(s): Ehira, S.
  • Organizer: Physiology and Biotechnology of Microalgae
  • Place of Presentation: Moscow, Russia
• [Presentation] 糸状性シアノバクテリアを用いた細胞間分業による効率的バイオ燃料生産 (2012)
  • Author(s): 得平茂樹, 大森正之
  • Organizer: 第64回日本生物工学会大会
  • Place of Presentation: 神戸
• [Presentation] Regulation of compatible solute synthesis in the nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120 (2012)
  • Author(s): 得平茂樹, 大森正之
  • Organizer: 第28回日本微生物生態学会大会
  • Place of Presentation: 愛知
• [Presentation] Regulation of glycogen metabolism in the nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120 (2012)
  • Author(s): Ehira, S., and Ohmori, M.
  • Organizer: 14^<th> International Symposium on Phototrophic Prokaryotes
  • Place of Presentation: Porto, Portugal
• [Presentation] シアノバクテリアにおいてチオレドキシンの転写を制御するレドックス応答性転写因子RexT (2012)
  • Author(s): 得平茂樹, 大森正之
  • Organizer: 第3回日本光合成学会年会
  • Place of Presentation: 横浜
• [Presentation] 窒素固定型シアノバクテリアAnabaena sp. PCC 7120における炭素・窒素代謝制御 (2012)
  • Author(s): 得平茂樹, 大森正之
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都
• [Presentation] シアノバクテリアにおける細胞分化に伴う糖代謝系変換制御 (2012)
  • Author(s): 得平茂樹, 大森正之
  • Organizer: 第7回日本ゲノム微生物学会年会
  • Place of Presentation: 東京
• [Presentation] シアノバクテリアにおいてチオレドキシンの転写を制御するレドックス応答性転写因子RexT (2012)
  • Author(s): 得平茂樹，大森正之
  • Organizer: 日本光合成学会
  • Place of Presentation: 東京工業大学（神奈川）
• [Presentation] Regulation of compatible solute synthesis in the nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120 (2012)
  • Author(s): 得平茂樹，大森正之
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 豊橋技術科学大学（愛知）
• [Presentation] 糸状性シアノバクテリアを用いた細胞間分業による効率的バイオ燃料生産 (2012)
  • Author(s): 得平茂樹，大森正之
  • Organizer: 日本生物工学会
  • Place of Presentation: 神戸国際会議場（兵庫）
• [Presentation] Regulation of glycogen metabolism in the nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120 (2012)
  • Author(s): Ehira, S., and Ohmori, M
  • Organizer: 14th International Symposium on Phototrophic Prokaryotes
  • Place of Presentation: Porto, Portugal
• [Presentation] 窒素固定型シアノバクテリアAnabaena sp. PCC 7120における炭素・窒素代謝制御 (2012)
  • Author(s): 得平茂樹、大森正之
  • Organizer: 日本植物生理学会
  • Place of Presentation: 京都産業大学(京都府)
• [Presentation] シアノバクテリアにおける細胞分化に伴う糖代謝系変換制御 (2012)
  • Author(s): 得平茂樹、大森正之
  • Organizer: 日本ゲノム微生物学会
  • Place of Presentation: 立教大学(東京都)
• [Presentation] Regulation of glycogen metabolism in the nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120 (2011)
  • Author(s): Ehira, S.
  • Organizer: The 6^<th> German-Japanese Binational Seminar
  • Place of Presentation: Freiburg-Munzingen, Germany
• [Presentation] 窒素固定型シアノバクテリアにおける炭素・窒素代謝制御 (2011)
  • Author(s): 得平茂樹, 大森正之
  • Organizer: 第27回日本微生物生態学会大会
  • Place of Presentation: 京都
• [Presentation] Regulation of carbon and nitrogen metabolism in cyanobacteria (2011)
  • Author(s): Ehira, S., and Ohmori, M.
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo, Japan
• [Presentation] 窒素固定型シアノバクテリアにおける糖代謝制御 (2011)
  • Author(s): 得平茂樹, 大森正之
  • Organizer: 第2回日本光合成学会年会
  • Place of Presentation: 京都
• [Presentation] 窒素固定型シアノバクテリアにおける炭素・窒素代謝制御 (2011)
  • Author(s): 得平茂樹、大森正之
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 京都大学(京都府)
• [Presentation] 窒素固定型シアノバクテリアにおける糖代謝制御 (2011)
  • Author(s): 得平茂樹、大森正之
  • Organizer: 日本光合成学会
  • Place of Presentation: 京都大学(京都府)
• [Presentation] Regulation of glycogen metabolism in the nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120 (2011)
  • Author(s): Ehira Shigeki
  • Organizer: The 6th German-Japanese Binational Seminar
  • Place of Presentation: Freiburg-Munzingen, Germany
• [Presentation] Regulation of carbon and nitrogen metabolism in cyanobacteria (2011)
  • Author(s): Ehira Shigeki, Ohmori Masayuki
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌コンベンションセンター(北海道)
• [Presentation] シアノバクテリアにおいて糖代謝を制御するレスポンスレギュレーターNrrAの活性制御
  • Author(s): 得平茂樹，藤木耕平，西山英里
  • Organizer: 第4回日本光合成学会年会
  • Place of Presentation: 名古屋
• [Presentation] The orphan response regulator NrrA controls glycogen catabolism in cyanobacteria
  • Author(s): 得平茂樹，西山英里，藤木耕平
  • Organizer: 第8回日本ゲノム微生物学会年会
  • Place of Presentation: 東京
• [Presentation] ヘテロシスト形成に伴う遺伝子発現とチラコイド膜光化学系変化の同時タイムラプス顕微分光測定
  • Author(s): 野末秀穂，佐藤直樹，豊島正和，得平茂樹，寺嶋正秀，熊崎茂一
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山
• [Presentation] Anabaena sp. PCC 7120におけるペントースリン酸経路構成遺伝子の転写制御機構
  • Author(s): 西山英里，中村康太，日原由香子，得平茂樹
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山
• [Presentation] Regulation of sucrose synthesis in the nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120
  • Author(s): 得平茂樹，木村聡，大森正之
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山
• [Presentation] Regulation of sugar metabolism in the nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120
  • Author(s): Ehira, S.
  • Organizer: 18th International Congress on Nitrogen Fixation
  • Place of Presentation: Miyazaki
• [Book] Spirulina : an example of cyanobacteria as nutraceuticals (In Sharma, N.K., Stal, L.J., Rai, A.K. (ed) Cyanobacteria : An Economic Perspective.) (2014)
  • Author(s): Ohmori, M., Ehira, S.
  • Publisher: John Wiley & Sons, Ltd.
• [Book] 第2章藍藻(シアノバクテリア)のゲノム解析と生理機能, (『微細藻類によるエネルギー生産と事業展望』) (2012)
  • Author(s): 得平茂樹, 大森正之, 竹山春子監修
  • Publisher: シーエムシー出版
• [Remarks]
  • URL: http://www.biol.se.tmu.ac.jp/labo.asp?ID=molgen