| Project/Area Number |
23612004
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Mechanobiology
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| Research Institution | Okayama University |
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Principal Investigator |
INAGAKI Junko 岡山大学, 医歯(薬)学総合研究科, 助教 (90271056)
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| Co-Investigator(Kenkyū-buntansha) |
IROHATA Satoshi 岡山大学, 国際センター, 准教授 (90332791)
NINOMIYA Yoshihumi 岡山大学, 医歯薬学総合研究科, 教授 (70126241)
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| Co-Investigator(Renkei-kenkyūsha) |
NARUSE Keiji 岡山大学, 医歯薬学総合研究科, 教授 (40252233)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | ADAMTS1 / 細胞外マトリックス / がん内圧 / リンパ管新生 / 血管新生 |
|---|
| Research Abstract |
ADAMTS1 is a matrix metalloproteinase and inhibits angiogenesis. We investigated the effect of ADAMTS1 on lymphangiogenesis. We further examined the relationship between tumor interstitial fluid pressure (TIFP) and tumor growth. We demonstrated that overexpressed-ADAMTS1 transfectants bind to VEGFC and made a complex and dampened the phosphorylation of VEGFR3, inhibiting lymphangiogenesis. Moreover, when VEGFC was injected to enhance lymphangiogenesis in a xenograft mouse model, TIFP was reduced and tumor growth was also attenuated. Finally, we stretched HMVEC-dLys and examined lymphangiogenesis-related genes. Mechanical stress may be involved in the regulation of lymphangiogenesis-related gene expressions in lymphatic endothelial cells.
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