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Total photon flux measurement of dim LED reference light source

Research Project

Project/Area Number 23615013
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Reliable environmental measurement methods
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

KAZUKI Niwa  独立行政法人産業技術総合研究所, 計測標準研究部門, 主任研究員 (30443211)

Co-Investigator(Kenkyū-buntansha) KATO Dai-ichiro  兵庫県立大学, 大学院工学研究科 (60423901)
Co-Investigator(Renkei-kenkyūsha) NAKAYA Takanori  浜松ホトニクス株式会社, 電子管事業部 (60600690)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords光放射測定 / 分光計測 / LED / 積分球 / 全放射束 / 生物発光 / 量子収率 / 微弱光 / 分光放射測定 / レポータージーンアッセイ / 毒性試験 / 微弱光特性評価
Research Abstract

For the purpose of quantitative photon measurements in bioluminescence assays, we measured photon number emitted from reference light sources. For precise and reliable absolute measurements, we evaluated uncertainty of our integrating sphere spectral radiometer in accordance with the methodologies for spectral radiometry standards. This integrating sphere system had been specially designed and constructed to measure quite low level intensity of photons from bioluminescence reaction and calibrated using a national standard traceable spectral irradiance standard lamp. We also investigate quantum yield and other enzyme parameters of mutant luciferases to contribute the understanding the biochemistry of firefly bioluminescence reaction. These quantitative basis of photon measurement will contribute the applications of bioluminescence reaction, which is increasingly employed as gene expression marker to monitor status of cellular activity.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (24 results)

All 2013 2012 2011

All Journal Article (9 results) (of which Peer Reviewed: 6 results) Presentation (14 results) Book (1 results)

  • [Journal Article] Interconversion of ketoprofen recognition in firefly luciferase-catalyzed enantioselective thioesterification reaction using from Pylocoeria miyako (PmL) and Hotaria parvura (HpL) just by mutating two amino acid residues2013

    • Author(s)
      D. Kato, Y. Hiraishi, M. Maenaka, K. Yokoyama, K. Niwa, et al
    • Journal Title

      Journal of Biotechnology

      Volume: 168(3) Pages: 277-283

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cloning and characterization of luciferase from a Fijian luminous click beetle.2013

    • Author(s)
      Mitani Y, Futahashi R, Niwa K, Ohba N, Ohmiya Y:
    • Journal Title

      Photobiology and Photochemistry

      Volume: 89 Issue: 5 Pages: 1163-1169

    • DOI

      10.1111/php.12097

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] In Vivo Imaging Demonstrates ATP Release from Murine Keratinocytes and Its Involvement in Cutaneous Inflammation after Tape Stripping2013

    • Author(s)
      T. Takahashi, Y. Kimura, K. Niwa, et al.
    • Journal Title

      Journal of Investigative Dermatology

      Volume: 133(10) Pages: 2407-2415

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Confirmation of color determination factors for Ser286 derivatives of firefly luciferase from Luciola cruciata (LUC-G)2013

    • Author(s)
      D. Kato, T. Kubo, M. Maenaka, K. Niwa, et al.
    • Journal Title

      Journal of Molecular Catalysis B:Enzymatic

      Volume: 87 Pages: 18-23

    • DOI

      10.1016/j.molcatb.2012.10.009

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Interconversion of ketoprofen recognition in firefly luciferase-catalyzed enantioselective thioesterification reaction using from Pylocoeria miyako (PmL) and Hotaria parvura (HpL) just by mutating two amino acid residues2013

    • Author(s)
      Dai-ishiro Kato, Kazuki Niwa, et al.
    • Journal Title

      JOURNAL OF BIOTECHNOLOGY

      Volume: 168-3 Issue: 3 Pages: 277-283

    • DOI

      10.1016/j.jbiotec.2013.04.024

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vivo imaging demonstrates ATP release from murine keratinocytes and its involvement in cutaneous inflammation after tape-stripping2013

    • Author(s)
      Takahashi, T., Kimura, Y., Niwa, K., Ohmiya, Y., Fujimura, T., Yamasaki, K., and Aiba, S.
    • Journal Title

      J Invest Dermatol

      Volume: 133 Issue: 10 Pages: 2407-15

    • DOI

      10.1038/jid.2013.163

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ホタルの生物発光反応に関する未解明課題への挑戦2012

    • Author(s)
      丹羽一樹
    • Journal Title

      日本MRSニュース

      Volume: 24(3) Pages: 6-8

    • Related Report
      2013 Final Research Report
  • [Journal Article] ホタルの生物発光反応に関する未解明課題への挑戦2012

    • Author(s)
      丹羽一樹
    • Journal Title

      日本MRSニュース

      Volume: 24-3 Pages: 6-8

    • Related Report
      2012 Research-status Report
  • [Journal Article] Measurement of Angular Nonuniformity of an Integration Sphere under 2 Illumination Geometry2011

    • Author(s)
      神門 賢二、丹羽 一樹、座間 達也、Yuqin Zong、Yoshi Ohno
    • Journal Title

      Proceedings of NEWRAD 2011

      Volume: - Pages: 284-287

    • Related Report
      2011 Research-status Report
  • [Presentation] Firefly luciferase bioluminescence activity profile based on quantum yield and kinetics2013

    • Author(s)
      K. Niwa, Y. Ichino, D. Kato, et al.
    • Organizer
      Biotrance2013
    • Place of Presentation
      Manchester
    • Related Report
      2013 Final Research Report
  • [Presentation] Active site environmental effects on the catalytic activities of firefly luciferase2013

    • Author(s)
      D. Kato, M. Maenaka, K. Takaya, K. Niwa, et al.
    • Organizer
      Biotrance2013
    • Place of Presentation
      Manchester
    • Related Report
      2013 Final Research Report
  • [Presentation] Firefly Luciferases Bioluminescence Activity Profile Based on Quantum Yield and Kinetics2013

    • Author(s)
      Kazuki Niwa, Yoshiro Ichino, Dai-ishiro Kato, et al.
    • Organizer
      BioTrans2013
    • Place of Presentation
      マンチェスター大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Active site environment effects on the catalytic activities of firefly luciferase2013

    • Author(s)
      Dai-ishiro Kato, Mika Maenaka, Takaya Kubo, Kazuki Niwa, et al.
    • Organizer
      BioTrans2013
    • Place of Presentation
      マンチェスター大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Measurement of Angular Nonuniformity of an Integrating Sphere for Total Radiant Spectral Radiant Flux Measurement2012

    • Author(s)
      K. Niwa, et al
    • Organizer
      CIE2012
    • Place of Presentation
      杭州
    • Year and Date
      2012-09-20
    • Related Report
      2013 Final Research Report
  • [Presentation] Quantum yield and kinetics of bioluminescence reaction using various beetle luciferases2012

    • Author(s)
      K. Niwa, Y. Ichino, Y. Nakajima, D. Kato, et al.
    • Organizer
      ISBC2012
    • Place of Presentation
      Guelph/Canada
    • Related Report
      2013 Final Research Report
  • [Presentation] Color tuning of firefly luciferase bioluminescence by hydroxy bonding network customization around the active site2012

    • Author(s)
      M. Maenaka, D. Kato, T. Kubo, K. Niwa, et al.
    • Organizer
      ISBC2012
    • Place of Presentation
      Guelph/Canada
    • Related Report
      2013 Final Research Report
  • [Presentation] MEASUREMENT OF ANGULAR NONUNIFORMITY OF AN INTEGRATION SPHERE FOR TOTAL SPECTRAL RADIANT FLUX MEASUREMENT2012

    • Author(s)
      丹羽 一樹、神門 賢二、座間 達也、ユージン、オーノ ヨシ
    • Organizer
      CIE2012
    • Place of Presentation
      杭州/中国
    • Related Report
      2012 Research-status Report
  • [Presentation] Quantum yield and kinetics of the bioluminescence reaction using various beetle luciferases2012

    • Author(s)
      丹羽 一樹、市野 善朗、中島 芳浩、加藤 太一郎、平石 善洋、近江谷 克裕
    • Organizer
      17th ISBC
    • Place of Presentation
      Guelph/Canada
    • Related Report
      2012 Research-status Report
  • [Presentation] Measurement of Angular Nonuniformity of an Integration Sphere under 2π Illumination Geometry2011

    • Author(s)
      神門 賢二、丹羽 一樹、座間 達也、Yuqin Zong、Yoshi Ohno
    • Organizer
      NEWRAD2011
    • Place of Presentation
      米国、ハワイ州、マウイ島グランドワイレアホテル
    • Related Report
      2011 Research-status Report
  • [Presentation] ゲンジボタル由来ルシフェラーゼ活性中心Ser286変異体の発光活性解析2011

    • Author(s)
      久保貴矢, 加藤太一郎, 前中美華, 丹羽一樹, 近江谷克裕, 武尾正弘, 根来誠司
    • Organizer
      生物発光化学発光研究会第28回学術講演会
    • Place of Presentation
      長崎県、長崎大学文京キャンパス
    • Related Report
      2011 Research-status Report
  • [Presentation] ゲンジボタル由来ルシフェラーゼ活性中心における酵素-基質間相互作用2011

    • Author(s)
      前中美華, 加藤太一郎, 丹羽一樹, 近江谷克裕, 武尾正弘, 根来誠司
    • Organizer
      生物発光化学発光研究会第28回学術講演会
    • Place of Presentation
      長崎県、長崎大学文京キャンパス
    • Related Report
      2011 Research-status Report
  • [Presentation] ホタル生物発光反応の量子収率測定2011

    • Author(s)
      丹羽一樹, 市野善朗, 中島芳浩,加藤太一郎, 近江谷克裕
    • Organizer
      生物発光化学発光研究会第28回学術講演会
    • Place of Presentation
      長崎県、長崎大学文京キャンパス
    • Related Report
      2011 Research-status Report
  • [Presentation] 活性部位周辺の水素結合ネットワークに着目したホタルルシフェラーゼの発光色制御2011

    • Author(s)
      前中美華, 加藤太一郎, 久保貴矢, 丹羽一樹, 近江谷克裕, 武尾正弘, 根来誠司
    • Organizer
      第15 回生体触媒化学シンポジウム
    • Place of Presentation
      東京都、慶應大学薬学部芝共立キャンパス
    • Related Report
      2011 Research-status Report
  • [Book] バイオテクノロジー領域のISO/TC新設の動き、幹細胞技術の標準化―再生医療への期待(ISBN978-4-542-3019-7)2012

    • Author(s)
      丹羽一樹, 湯元昇
    • Publisher
      日本規格協会
    • Related Report
      2013 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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