| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
|---|
| Research Abstract |
To understand the role of histone H3K4 demethylase KDM1B for oocyte-specific imprinting, expression of KDM1B during oocyte growth was analyzed and the establishment of methylation imprints in Zac1 and Mest loci were examined under conditions of DNA methyltransferases (DNMTs) overexpression. The results showed that KDM1B mRNA was expressed at a high level in oocytes from the non-growing stage but KDM1B protein was expressed from the growth stage onward. Excess of DNMTs induced early acquisition of DNA methylation imprints at Zac1 but not Mest in early-growing oocytes. Thus, we revealed that the presence of DNMTs and KDM1B is essential but not sufficient for establishment of oocyte-specific imprinting.
|
