| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
|---|
| Research Abstract |
The possible new nutritional functions of sphingolipids are proposed in this study, by analyzing the composition of exosomes prepared from sphingomyelin (SPM)-treated hepatoma cells or SPM-intubated rat sera. The treatment with SPM of human hepatoma cells resulted in the decrease of proliferation. The conditioned media of SPM-treated hepatoma showed an anti-proliferative effect on hepatoma cells. SPM treatment caused the change in miRNA composition of secreted exosome, thus suppressed the proliferation of surrounding cells. The exosomes prepared from SPM-intubated rat sera significantly promoted the glucose uptake of cultured muscle cells. This result suggests the possibility that orally-intubated SPM affects the composition of serum exosomes and shows some physiological effects in vivo.
These results clearly show the possibility that sphingolipids can affect the composition of exosomes secreted from cells or into sera, thus suggesting novel nutritional functions of sphingolipids.
|
